Aktivitas Antibakteri Ekstrak Etanol Daun Kapas (Gossypium barbadense L.) terhadap Staphylococcus epidermidis dan Propionibacterium acnes

Khumaidi, Akhmad; Nugrahani, Arsa Wahyu; Gunawan, Febriani

doi:10.24843/jfu.2020.v09.i01.p08

Cited by 4 publications

(8 citation statements)

References 10 publications

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“…The activity test begins with the preparation of MHA media 14 , then the P.acnes bacteria are inoculated 10 . After that, a 1 McFarlan or 10 8 CFU/ml bacterial suspension was made 15 . Antibacterial activity testing used the paper disc diffusion method (Kirby-Bauer Methode) with three different treatments of incubation time (7 days, 10 days, and 14 days) and variations of turmeric rhizome (10%, 15%, and 20%).…”

Section: Fermented Antibacterial Activity Testmentioning

confidence: 99%

Formulation and Evaluation of a Turmeric Kombucha Facial Toner with Potential as an Anti-Acne Agent

Muhsinin

Salsabilla

Mardhiani

et al. 2023

J. Drug Delivery Ther.

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Propionibacterium acnes is one of the gram-positive bacteria that causes acne. Many acne treatments use synthetic drugs that have side effects. One alternative for treatment is using fermented products such as kombucha. The potential of kombucha as an anti-acne can be increased by adding turmeric during the fermentation process. The product is Turmeric Kombucha, which will be applied to the skin. The cosmetic dosage form developed is Facial Toner. The research to be carried out aims to formulate and test the activity of turmeric kombucha facial toner preparations against P.acnes bacteria. The novelties of this research include increasing the anti-acne activity of Turmeric Kombucha and the addition of Turmeric Kombucha as an active ingredient in facial toner preparations that meet the evaluation requirements, activity tests, and stability tests. The stages of the method started with Turmeric Kombucha fermentation with variations in turmeric concentration and fermentation time. The fermentation results will be tested for antibacterial activity using the paper disc diffusion method. Turmeric Kombucha, which has the highest activity, is then used as an active substance in facial toner preparations, which will be evaluated and tested for stability. The results of the evaluation of facial toner preparations, all formulas meet the requirements for organoleptic, pH, and viscosity tests. The stability test results for facial toner preparations show that F3 is a stable formula at room temperature storage and extreme temperatures. Based on the results of the irritation test, kombucha turmeric facial toner did not irritate. The antibacterial test results showed that all formulas could inhibit P.acnes bacteria with F3 as the formula with the most effective inhibition diameter of 7.33 ± 0.57 mm, then F1 and F4 6.33 ± 0.57 mm, and F2 6 ± 0 mm. This study concludes that fermented turmeric kombucha can be formulated as an anti-acne facial toner. Keywords: paper disc diffusion, Propionibacterium acnes, facial toner, Turmeric Kombucha

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Section: Fermented Antibacterial Activity Testmentioning

confidence: 99%

Formulation and Evaluation of a Turmeric Kombucha Facial Toner with Potential as an Anti-Acne Agent

Muhsinin

Salsabilla

Mardhiani

et al. 2023

J. Drug Delivery Ther.

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“…Patogenesis acne bersifat multifaktorial, terjadi keratinisasi folikel yang abnormal, peningkatan produksi sebum akibat hiperandrogenisme, proliferasi bakteri dan inflamasi (Kraft & Freiman, 2011). Staphylococcus epidermidis, Staphylococcus aureus, dan Propionibacterium acnes merupakan bakteri yang dapat menyebabkan timbulnya jerawat (Khumaidi et al, 2020;Kumar et al, 2016). Bakteri tersebut dapat menghidrolisis lemak, yaitu memecah asam lemak bebas pada lipid kulit sehingga menimbulkan peradangan yang dapat menyebabkan bakteri berproliferasi dan memperparah lesi jerawat (Khumaidi et al, 2020).…”

Section: Pendahuluanunclassified

“…Staphylococcus epidermidis, Staphylococcus aureus, dan Propionibacterium acnes merupakan bakteri yang dapat menyebabkan timbulnya jerawat (Khumaidi et al, 2020;Kumar et al, 2016). Bakteri tersebut dapat menghidrolisis lemak, yaitu memecah asam lemak bebas pada lipid kulit sehingga menimbulkan peradangan yang dapat menyebabkan bakteri berproliferasi dan memperparah lesi jerawat (Khumaidi et al, 2020). Terapi antibiotik telah menjadi bagian pengobatan jerawat selama bertahuntahun, akan tetapi penggunaan antibiotik yang luas dapat menyebabkan munculnya bakteri resisten.…”

Section: Pendahuluanunclassified

AKTIVITAS ANTIAKTERI EKSTRAK ETANOL BIJI DAN BUAH KUPA (Syzygium polichepalum (miq.) Merr.& perry) TERADAP BAKTERI PENYEBAB JERAWAT

Aryani¹,

Hazar²,

Mardliyani³

2023

JIFF

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merupakan penyakit inflamasi kronis dan multifaktorial pada kulit dan unit pilosebasea yang dapat disebabkan oleh bakteri. Penelitian ini dilakukan untuk mengetahui potensi antibakteri ekstrak etanol biji dan daging buah kupa (Syzygium polycephalum) terhadap bakteri penyebab jerawat, yaitu Propionibacterium acnes, Staphylococcus epidermidis, dan Staphylococcus aureus. Pada penelitian ini dilakukan uji aktivitas antibakteri dengan menentukan nilai konsentrasi hambat minimum (KHM) dan diameter hambat ekstrak etanol biji dan daging buah kupa terhadap bakteri P. acnes, S. epidermidis, dan S. aureus menggunakan metode difusi agar. Hasil menunjukkan bahwa nilai KHM dan diameter hambat ekstrak etanol biji buah kupa terhadap bakteri P. acnes (1,56%; 8,9 ± 0,01 mm); S. epidermidis (0,78%; 8,3 ± 0,01 mm) dan S. aureus (3,13%; 8,5 ± 0,02 mm), sedangkan nilai KHM dan diameter hambat antibakteri ekstrak etanol daging buah kupa terhadap bakteri P. acnes (12,5%; 13,1 ± 0,03 mm); S. epidermidis (25%; 12,1 ± 0,09 mm) dan S. aureus (0,78%; 10,0 ± 0,03 mm). Potensi aktivitas ekstrak etanol biji dan daging buah kupa terhadap bakteri P. acnes, S. epidermidis, dan S. aureus termasuk ke dalam kategori sedang hingga kuat.

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“…This study's determination of lymphocyte proliferation refers to the Khumaidi et al (2020) procedure with some modifications. Isolation of lymphocyte cells was obtained from the extraction of the mice's spleen.…”

Section: Lymphocyte Cell Proliferation Testmentioning

confidence: 99%

“…The study by Khumaidi et al (2020) showed that methanol extract, n-hexane fraction, ethyl acetate, and Begonia medicinal water increased lymphocyte cell proliferation (immunostimulator). The sample extract contains flavonoids that can increase the production of IFN-γ but can reduce Th2 cytokines (IL-4) so that it can increase lymphocyte proliferation.…”

Section: Lymphocyte Cell Proliferation Testmentioning

confidence: 99%

In Vitro Immunomodulatory Activity of Fig Fruit Ethanol Extract (Ficus carica Linn) against Phagocytosis Macrophages and Lymphocyte Proliferation

Azizah

Winanta²

2022

Trad.Med.J

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Fig (Ficus carica L.) is a natural product that potentially can improve the immune system because it has flavonoids that have the potential as immunostimulants. The research aims to determine the possibility of fig fruit ethanol extract as an immunomodulator. Immunomodulatory activity is determined by knowing the activity of macrophage phagocytosis and lymphocyte proliferation in vitro and the levels of flavonoids in the extract. The research began with extraction, and then the sample was tested with TLC and colorimetry methods. Furthermore, the sample in the immunomodulatory activity test in vitro was measured through the activity of macrophage phagocytosis and lymphocyte proliferation. In the phagocytosis activity test, macrophage cells were given samples in various concentrations and latex beads. The number of activated macrophages and the number of latex phagocyted by the macrophage is then calculated. For tests of lymphocyte proliferation activity, lymphocyte cells were sampled with different concentrations and induced hepatitis B vaccine. Then the cell absorbance was read with an Elisa reader at 550nm wavelength. The study results found that the samples contained flavonoid compounds, and the total flavonoid levels obtained were 0.74±0.01 mgEQ/g samples. The immunomodulatory activity showed that the sample increased phagocytosis activity of macrophages compared to cell control. The lymphocyte proliferation test produced stimulation index<2 values, showing no effect on the proliferation of lymphocytes. This study indicated that fig fruit ethanol extract could increase the phagocytosis activity of macrophage cells but did not affect the proliferation of lymphocyte cells in vitro.

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Aktivitas Antibakteri Ekstrak Etanol Daun Kapas (Gossypium barbadense L.) terhadap Staphylococcus epidermidis dan Propionibacterium acnes

Cited by 4 publications

References 10 publications

Formulation and Evaluation of a Turmeric Kombucha Facial Toner with Potential as an Anti-Acne Agent

Formulation and Evaluation of a Turmeric Kombucha Facial Toner with Potential as an Anti-Acne Agent

AKTIVITAS ANTIAKTERI EKSTRAK ETANOL BIJI DAN BUAH KUPA (Syzygium polichepalum (miq.) Merr.& perry) TERADAP BAKTERI PENYEBAB JERAWAT

In Vitro Immunomodulatory Activity of Fig Fruit Ethanol Extract (Ficus carica Linn) against Phagocytosis Macrophages and Lymphocyte Proliferation

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