2012
DOI: 10.4236/abb.2012.32018
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<i>In vitro</i> propagation of <i>Aegle marmelos</i> (L.) corr., a medicinal plant through axillary bud multiplication

Abstract: A protocol for rapid in vitro propagation from nodal explants of the medicinal tree species Aegle marmelos (L.) corr. of family Rutaceae has been described. High frequency bud break were induced on Murashige and Skoog's (1962) basal medium supplemented with 0.5 mg benzyladenine (BA)/l. After 10 days of culture, nodal explants with multiplied buds started callusing with restricted growth and defoliation. When the same nodal explants ware transferred into the same basal medium supplemented with 0.5 mg BA/l with … Show more

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Cited by 14 publications
(4 citation statements)
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“…Irrespective of explants the treatment T 2 (Medium + 0.5mg/lit KN + 0.5mg/lit BAP + 0.5mg/lit IAA), was considered to be best than other treatments for shoot induction. This was accordance with result of Puhan and Rath (2012) in bael, reported that when nodal explants ware transferred into the same basal medium supplemented with 0.5 mg BA/l with different concentrations of either kinetin (KN) or gibberellic acid (GA3) or in combinations has shown healthy shoots with expanded shoot length. T 2 was observed as best treatment for number of shoots and irrespective of treatment the cotyledonary explant was found to be best in emergence of number of shoots and the number of shoots emerged at higher concentration of BAP was less than lower concentration of BAP (Table 2).…”
Section: Resultssupporting
confidence: 91%
“…Irrespective of explants the treatment T 2 (Medium + 0.5mg/lit KN + 0.5mg/lit BAP + 0.5mg/lit IAA), was considered to be best than other treatments for shoot induction. This was accordance with result of Puhan and Rath (2012) in bael, reported that when nodal explants ware transferred into the same basal medium supplemented with 0.5 mg BA/l with different concentrations of either kinetin (KN) or gibberellic acid (GA3) or in combinations has shown healthy shoots with expanded shoot length. T 2 was observed as best treatment for number of shoots and irrespective of treatment the cotyledonary explant was found to be best in emergence of number of shoots and the number of shoots emerged at higher concentration of BAP was less than lower concentration of BAP (Table 2).…”
Section: Resultssupporting
confidence: 91%
“…For the induction of strong and stout root systems the elongated shoots were individually cultured on half strength MS medium with 1.0 mg/l IBA which gave the best response with 91.60 % of roots produced and on an average of 22.83 roots per shoot were recorded within 4 weeks. The effectiveness of IBA in rooting has been reported for many medicinal plants, viz; Aegle marmelos (Puhan and Rath 2012), Alpinia zerumbet (Rakkimuthu et al 2011), Clitoria ternatea L. (Barik et al 2007), Murraya koenigii (L.) Spreng (Bhuyan et al 1997) and Ocimum basilicum L. (Sahoo et al 1997). This is due to an imbalance between endogenous auxin and exogenously supplied auxin IBA (Manickam et al 2000).…”
Section: Discussionmentioning
confidence: 99%
“…The fact may be attributed that the use of juvenile tissues to increase enzymatic activity at the cotyledonary node zone as also observed in case of bean 20 . Successful shoot regeneration from cotyledon node segments of A. marmelos has been reported earlier [21][22][23][24] . The regeneration system can be adopted for mass production due to readily available immature seed embryos maybe a starting point for the development of genetic …”
Section: Resultsmentioning
confidence: 98%