Analysis of Bioactive compounds from methanol extract of Diadema setosum sea urchin gonads using gas Chromatography-mass spectrometry

Tulandi, Silvester Maximus; Tanzil, Lisawati; Ulfa, Dian Maria

doi:10.5958/0974-360x.2021.00289.4

Cited by 4 publications

(2 citation statements)

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“…Previous studies have generally been concerned with species registration, availability, distribution in the region, biological properties and extraction of chitin and chitosan (Rahman et al, 2012;Fitriyani et al, 2022;Uğurlu & Duysak, 2022). Furthermore, there are studies on the components of nutrients (protein, lipid, fatty acid), antibacterial effects, cytotoxic activity, and accumulation of metals in tissues consumed such as gonads of this species (Flammang et al, 1997;Marimuthu et al, 2015;Tulandi et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Collagen from the Invasive Sea Urchin (Diadema setosum L., 1778) in North-Eastern Mediterranean Sea, Türkiye

Uğurlu

Duysak

Sayın

et al. 2023

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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In the present study, collagen was obtained from the tissues of invasive sea urchin (Diadema setosum) rapidly spreading in the Mediterranean. As a result, the yield of collagen isolated from sea urchin was determined to be 23.78±1.33% (dry weight). According to the SDS-Page (Sodium Dodecyl Sulfate-Polyacrylamide gel electrophoresis) analysis, it contains (α1)2α2, (Molecular Weight (MW) 124, 114 kDa) resembling the calfskin collagen and ß chains (MW 245 kDa), similar to the of the sea urchin collagen. In the amino acid analysis of collagen, it was determined that the imino acid (proline+hydroxyproline) content was 196.1 residues/1000 residues. Amide A (3301 cm-1), B (2924 cm-1), I (1643 cm-1), II (1550 cm-1), and III (1242 cm-1) functional bands were visualized in the FTIR spectrum. These results were similar to other collagen sources. For the first was used as a source of collagen in the present study. An alternative collagen source to mammalian collagen, which is used commercially in many industries such as biomedicine, food, and cosmetics, was isolated for the first time from D. setosum, and it was proposed that marine collagen can be used as an alternative collagen source and a functional component in areas including food, cosmetics, and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Collagen from the Invasive Sea Urchin (Diadema setosum L., 1778) in North-Eastern Mediterranean Sea, Türkiye

Uğurlu

Duysak

Sayın

et al. 2023

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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“…Therefore, natural drug discovery con rmed few anticancer medicines such as Erubilin mesylate, Trabectedin or Brentuximab vedotin from the marine natural products [11]. Furthermore, Sea urchin has various bioactive compounds such as cholesterol, glycol, alcohol, sterol, sitosterol, cardiolipin, phosphatidylinositol, phosphatidylserine, phosphatidylserine, and phosphatidylcholine and so on which have the crucial effect on different infectious diseases [12].…”

Section: Introductionmentioning

confidence: 99%

Compounds identified from the marine Sea Urchin (Diadema savignyi) as Potential Anti-Cancer Drug Candidate against Human Colorectal Cancer: A Bioinformatics Approaches

Molla

Aljahdali

2022

Preprint

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The occurrence of colorectal cancer is estimated to increase by 1.9 million people by 2020, making it the second most common chronic disease. Yet, no specific drug candidates for treating this cancer have been developed or made accessible. However, the nuclear transport receptor importin-11 transports β-catenin to the nucleus and regulates the proliferation of colorectal cancer cells. The inhibition of importin-11 can block the β-catenin nuclear import and the growth of APC-mutant colorectal cancer cells. As a result, this research aimed to discover natural anti-cancer drugs that can block the function of importin-11, inhibiting the progression of colorectal cancer. The structure of 19 compounds isolated from sea urchins was initially determined using gas chromatography-mass spectrometry (GC-MS). Consequently, a molecule docking, absorption, distribution, metabolism, and excretion (ADME) approach and a molecular dynamics (MD) simulation approach were used to screen the compounds. Four molecules were initially identified with PubChem: CID 11955, CID 605775, CID 608814, and CID 6432458. Pharmacokinetics and toxicity for all compounds have been evaluated. To confirm the stability of their binding to the target protein, each compound was assessed using MD simulation methods. An in silico method revealed the top four compounds that could have pharmacological interest with a higher affinity for the target protein. Altogether, we describe here that compounds from sea urchins show interesting anti-cancer activity against importin-11, which could potentially be used to develop an anti-CRC drug. Therefore, further experimental validation is recommended to ensure a comprehensive assessment of their mechanism of action.

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Phytochemical screening, biological evaluation, and molecular docking studies of aerial parts of Trigonella hamosa (branched Fenugreek)

Rao

Ahmad

Aati

et al. 2023

Arabian Journal of Chemistry

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Analysis of Bioactive compounds from methanol extract of Diadema setosum sea urchin gonads using gas Chromatography-mass spectrometry

Cited by 4 publications

References 0 publications

Isolation and Characterization of Collagen from the Invasive Sea Urchin (Diadema setosum L., 1778) in North-Eastern Mediterranean Sea, Türkiye

Isolation and Characterization of Collagen from the Invasive Sea Urchin (Diadema setosum L., 1778) in North-Eastern Mediterranean Sea, Türkiye

Compounds identified from the marine Sea Urchin (Diadema savignyi) as Potential Anti-Cancer Drug Candidate against Human Colorectal Cancer: A Bioinformatics Approaches

Phytochemical screening, biological evaluation, and molecular docking studies of aerial parts of Trigonella hamosa (branched Fenugreek)

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