Aniba rosaeodora (Var. amazonica Ducke) Essential Oil: Chemical Composition, Antibacterial, Antioxidant and Antitrypanosomal Activity

Teles, Amanda Mara; Silva-Silva, João Victor; Fernandes, Juan Matheus Pereira; Calabrese, Kátia da Silva; Abreu-Silva, Ana Lúcia; Marinho, Silvio Carvalho; Mouchrek, Adenilde Nascimento; Filho, Victor Elias Mouchrek; Almeida-Souza, Fernando

doi:10.3390/antibiotics10010024

Cited by 17 publications

(20 citation statements)

References 47 publications

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“…Wells with cells treated with LPS (Sigma-Aldrich, St Louis, MO, USA) at 10 µg/mL and with dexamethasone (Sigma-Aldrich, St Louis, MO, USA) at 100 µM. After 48 h of treatment, cell viability assay was performed using the colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (Sigma-Aldrich, St Louis, MO, USA) method [ 24 ], with modifications [ 25 ]. Briefly, 10 µL of MTT at 5 mg/mL was added and incubated for two hours at 37 °C and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Xavier

Teles

Tellis³

et al. 2021

Foods

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Açaí berry is a fruit from the tree commonly known as açaízeiro (Euterpe oleracea Mart.) originated from the Amazonian region and widely consumed in Brazil. There are several reports of the anti-inflammatory activity of its pulp and few data about the seed’s potential in inflammation control. This work aimed to evaluate the effect of catechin-rich açaí extract on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and carrageenan-induced paw edema. The treatment with E. oleracea ethyl acetate extract (EO-ACET) was used in an in vitro model performed with macrophages stimulated by LPS, in which pro-inflammatory markers were evaluated, and in an in vivo model of acute inflammation, in which edema inhibition was evaluated. EO-ACET showed an absence of endotoxins, and did not display cytotoxic effects in RAW 264.7 cells. LPS-stimulated cells treated with EO-ACET displayed low levels of nitrite and interleukins (IL’s), IL-1β, IL-6 and IL-12, when compared to untreated cells. EO-ACET treatment was able to inhibit carrageenan-induced paw edema at 500 and 1000 mg/kg, in which no acute inflammatory reaction or low mast cell counts were observed by histology at the site of inoculation of λ-carrageenan. These findings provide more evidence to support further studies with E. oleracea seeds for the treatment of inflammation.

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Section: Methodsmentioning

confidence: 99%

Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Xavier

Teles

Tellis³

et al. 2021

Foods

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“…[23] Moreover, Villamizar and co-workers demonstrated the trypanocidal activity of linalool against the trypomastigote forms of a T. cruzi clone Dm28c (IC 50 /24 h = 306 ng mL À 1 ), [24] whereas its activity against epimastigote and intracelular amstigote forms of T. cruzi (SC2005 strain) was reported by Teles and co-workers (IC 50 /24 h = 198.6 � 1.12 and 249.6 � 1.18 μg mL À 1 , respectively). [25] However, although the trypanocidal activity of LA-EO might be related to the high linalool content, synergism with other LA-EO components is possible and needs to be further investigated. Compound [a] RI exp [b] RT lit [c] % RA [d] DA RT lit [c] % RA [d] DA [a] Tentatively identified by comparison of their mass spectra with those mass spectra of the Wiley 7, NIST 08, and FFNSC 12 libraries and comparison of their experimental retention index (RI exp ) to literature (RI lit ) values.…”

Section: Resultsmentioning

confidence: 99%

Trypanocidal Activity of Dysphania ambrosioides, Lippia alba, and Tetradenia riparia Essential Oils against Trypanosoma cruzi

Crotti

Pagotti

Cândido

et al. 2021

Chemistry & Biodiversity

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Despite the current treatments against Chagas Disease (CD), this vector-borne parasitic disease remains a serious public health concern. In this study, we have explored the in vitro and/or in vivo trypanocidal and cytotoxic activities of the essential oils (EOs) obtained from Dysphania ambrosioides (L.) Mosyakin & Clemants (Amaranthaceae) (DA-EO), Lippia alba (Mill.) N.E. Brown (Verbenaceae) (LA-EO), and Tetradenia riparia (Hochst.) Codd (Lamiaceae) (TR-EO) grown in Brazil Southeast. DA-EO was the most active against the trypomastigote and amastigote forms in vitro; the IC 50 values were 8.7 and 12.2 μg mL À 1 , respectively. The EOs displayed moderate toxicity against LLCMK2 cells, but the DA-EO showed high selectivity index (SI) for trypomastigote (SI = 33.2) and amastigote (SI = 11.7) forms. Treatment with 20 mg/kg DA-EO, LA-EO, or TR-EO for 20 days by intraperitoneal administration reduced parasitemia by 6.36 %, 4.74 %, and 32.68 % on day 7 and by 12.04 %, 27.96 %, and 65.5 % on day 9. These results indicated that DA-EO, LA-EO, and TR-EO have promising trypanocidal potential in vitro, whereas TR-EO has also potential trypanocidal effects in vivo.

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“…The infected cells were treated with ravenelin (0.242–3.87 μM) and benznidazole (24.0–384.3 μM) at least in triplicate for 24 h. Coverslips with the infected and treated cells were fixed with Bouin solution stained with Giemsa solution and examined under light microscopy. The intracellular amastigote number of one hundred cells was normalized and used to calculate the IC 50 [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum

et al. 2021

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The natural compound ravenelin was isolated from the biomass extracts of Exserohilum rostratum fungus, and its antimicrobial, antiplasmodial, and trypanocidal activities were evaluated. Ravenelin was isolated by column chromatography and HPLC and identified by NMR and MS. The susceptibility of Gram-positive and Gram-negative bacteria strains to ravenelin was determined by microbroth dilution assay. Cytotoxicity was evaluated in hepatocarcinoma cells (HepG2) and BALB/c peritoneal macrophages by using MTT. SYBR Green I-based assay was used in the asexual stages of Plasmodium falciparum. Trypanocidal activity was tested against the epimastigote and intracellular amastigote forms of Trypanosoma cruzi. Ravenelin was active against Gram-positive bacteria strains, with emphasis on Bacillus subtilis (MIC value of 7.5 µM). Ravenelin’s antiparasitic activities were assessed against both the epimastigote (IC50 value of 5 ± 1 µM) and the intracellular amastigote forms of T. cruzi (IC50 value of 9 ± 2 µM), as well as against P. falciparum (IC50 value of 3.4 ± 0.4 µM). Ravenelin showed low cytotoxic effects on both HepG2 (CC50 > 50 µM) and peritoneal macrophage (CC50 = 185 ± 1 µM) cells with attractive selectivity for the parasites (SI values > 15). These findings indicate that ravenelin is a natural compound with both antibacterial and antiparasitic activities, and considerable selectivity indexes. Therefore, ravenelin is an attractive candidate for hit-to-lead development.

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Aniba rosaeodora (Var. amazonica Ducke) Essential Oil: Chemical Composition, Antibacterial, Antioxidant and Antitrypanosomal Activity

Cited by 17 publications

References 47 publications

Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Trypanocidal Activity of Dysphania ambrosioides, Lippia alba, and Tetradenia riparia Essential Oils against Trypanosoma cruzi

Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum

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