The objective of this paper is to study the combined effects of rib roughness and adverse pressure gradient produced in an asymmetric diffuser on turbulent flows. The two-dimensional asymmetric diffuser was comprised of a straight flat floor and a curved roof. The diffuser section was preceded and followed by straight parallel walls. The complete test conditions were comprised of a reference smooth floor and repeated arrays of transverse square ribs glued onto the floor to produce three pitch-to-height ratios, p / k = 3, 6, and 8. The curved roof was kept smooth in all the experiments. For each of the four test conditions, a particle image velocimetry was used to conduct detailed velocity measurements within the diverging section and also at locations upstream and downstream of the diverging section. From these measurements, the mean streamlines, mean velocities, turbulent intensities, Reynolds shear stress, and production terms in the transport equations for the turbulent kinetic energy and Reynolds stresses were obtained. The results obtained in the diverging section showed that the boundary layers that developed on the ribs thickened considerably at the expense of those adjacent to the roof opposite to the ribs. The streamlines and mean velocity profiles over the ribs showed that adverse pressure gradient increased the roughness sublayer substantially. Adverse pressure gradient and rib roughness also increased the drag and levels of the turbulent intensities, Reynolds shear stress and production terms compared to smooth-wall zero pressure gradient turbulent boundary layer. It appears, however, that adverse pressure gradient enhanced turbulence more effectively than it increased drag.