Antibacterial Activity of Bisanthraquinone (+)-1,1’-Bislunatin

Praptiwi,; Jamal, Yuliasri; Fathoni, Ahmad; Nurkanto, Arif; Agusta, Andria

doi:10.5454/mi.7.4.4

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…GNBP-10 was cultivated on Potato Dextrose Broth (PDB) (25 x 200 mL) medium and then extracted with ethyl acetate three times and dried under reduced pressure. The crude extract (1.21 g) was separated through Sephadex LH-20 (300 mL, Amersham Biosciences) column chromatography and eluted with methanol [5]. (+)-2,2'-Epicytoskyrin A appeared as fraction 1 (F1, 0.219 g) and was further purified by precipitation in 60 % methanol in the water at room temperature.…”

Section: Chemicalmentioning

confidence: 99%

Oral subchronic toxicity of (+)-2,2’epicytoskyrin a on male mice (Mus musculus)

et al. 2021

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(+)-2,2’Epicytoskyrin A (EpiA) is a secondary metabolite derived from an endophytic fungus Diaporthe sp. associated with the Gambier plant. EpiA has been reported to possess anticancer, antifungal, and antibacterial activities. In a mouse model, EpiA was also shown to low oral toxicity. Until now, there is no negative effect of EpiA reported yet. This study aimed to study the subchronic toxicity of this component in mice. This study used 60 male mice (Mus musculus) which were divided into four groups of treatments. Parameters that were analyzed were body weight, food consumption, blood chemicals, and histology. The results showed no significant difference in body weight gain and food consumption in all groups. Blood chemicals analysis showed increasing AST and ALT in dosage 12.5 mg/kg BW groups at day 90. Histological changes showed fatty degeneration and hydropic degeneration in the liver and mild inflammation in the kidney. No irreversible toxic effect of this component. So it can be beneficial for plant-based drug discovery. On the other hand, this study provides information about the safety of the development of EpiA as a drug candidate.

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Section: Chemicalmentioning

confidence: 99%

Oral subchronic toxicity of (+)-2,2’epicytoskyrin a on male mice (Mus musculus)

et al. 2021

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“…Hal ini disebabkan kandungan katekin pada gambir yang dapat berperan sebagai antibakteri. Menurut Praptiwi et al (2013) (Pambayun et al, 2007).…”

Section: Angka Lempeng Total (Alt)unclassified

Quality Profile of Smoked African Sharptooth Catfish (Clarias Gariepinus) Treated With Uncaria Gambier (Uncaria Gambir Roxb)

Sari

Agustini²,

Wijaya

et al. 2017

JDPI

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AbstrakPenelitian ini bertujuan untuk mengetahui sifat antibakterial gambir terhadap profil mutu ikan lele asap yang diberi perlakuan perendaman dalam larutan gambir serta untuk mengetahui pengaruh konsentrasi dan lama perendaman terhadap karakteristik fisika, kimia dan mikrobiologi. Metode penelitian menggunakan rancangan acak lengkap yang disusun secara faktorial dengan konsentrasi gambir faktor A (0%, 2%, 4% dan 6%), dan lama waktu perendaman faktor B (15, 30,dan 45 menit) sebagai perlakuan. Semua perlakuan dilakukan 3 replikasi. Profil mutu ikan lele asap yang diamati adalah Water activity (aw), kadar protein, tekstur, warna (L*lightness, C*chroma dan H*hue) dan Angka lempeng total (ALT). Hasil pengujian menunjukkan bahwa konsentrasi gambir dan lama waktu perendaman berpengaruh nyata terhadap profil mutu ikan lele asap. Profil mutu ikan lele asap hasil perlakuan adalah water activity (0,78-0,86), kadar protein (30,57-33,47%), tekstur (717,6-835,2 gf), Warna L* (27,8), C* (4,1-6,3%), H*(21,8-34,2 o ) dan ALT (2,36-5,49 Log Cfu/g). Perlakuan terbaik adalah perlakuan A4B3 (6%, 45 menit). Hasil Pengujian menunjukkan bahwa semua ikan lele asap yang diberi perlakuan perendaman dalam larutan gambir memiliki karakteristik yang lebih baik dibandingkan dengan kontrol (tanpa perendaman gambir).Kata kunci : gambir, konsentrasi, lama perendaman, mutu dan ALT. Protein content, Texture, color (L*lightness, C*chroma and H*hue) o ), ) and the best treatment was A4B3 (6% 45 m). All treatment showed the characteristic of smoked African sharptooth catfish which was introduced with gambier had better characteristic than control. Abstract The purpose of this research was to study antibacterial properties of gambier on the quality charecteristic of smoked African sharptooth catfish (Clarias gariepinus) which was marinated by antibacterial gambier, and to review the effect of concentration and marination time of gambier on the physical, chemical and microbiology characteristic of smoked African sharptooth catfish. Research applied completely randomized design with the concentration of gambier and marination time as treatment with 3 replication.The concentration of gambier consist of A1 (0%), A2 (2%), A3 (4%) and A4 (6%). The marination time consist of B1 (15 minute), B2 (30 minute) and B3 (45 minute). The parameters were Water activity (aw),

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“…Epi and bis retain a robust invitro antimycobacterial activity against M. tuberculosis h37rv, as indicated by MIC values 0.844 µM and 0.422 µM, respectively (18) . Additionally, the oral toxicity assays showed that both metabolites have low acute toxicity profiles (19) .Due to their immense biological activities, a certain amount of pure epi and bis compounds is needed to meet sufficient criteria for further research. The cultivation method that optimized the production of epi and bis has not been yet investigated.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Bis-anthraquinones Production from Endophytic Fungi Diaporthe sp. GNBP-10

Oktavia,

Evana,

Fahardita

et al. 2023

IJPS

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Bis-anthraquinones with a unique molecular backbone, (+)-2,2’-epicytoskyrin A (epi) and (+)-1,1′-bislunatin (bis), was produced by endophytic fungi Diaporthe sp GNBP-10 associated with Gambir plant (Uncaria gambier). Epi and bis possess robust antimicrobial activity toward various pathogens. This study focus on knowing the optimum condition of epi and bis production from Diaporthe sp GNBP-10. A series of culture media with various nutrient compositions was investigated in epi and bis production. The content of epi and bis was determined by measuring the area under the curve from TLC-densitometric (scanner) experiment. The linear regression analysis was then applied to obtain the results. The optimized epi and bis production was observed in liquid media 3 containing potato starch and dextrose with the amount of epi produced is 0.484mg while bis content is 0.163mg. The presence of carbohydrates, whether simple sugar or carbohydrate complex, plays an essential role in the bis-anthraquinones production from Diaphorthe sp culture. The presence of minerals and excessive protein sources did not significantly affect bis-anthraquinones production. Keywords: bis-anthraquinone, Diaporthe sp, culture media, regression, TLC scanner

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Antibacterial Activity of Bisanthraquinone (+)-1,1’-Bislunatin

Cited by 11 publications

References 12 publications

Oral subchronic toxicity of (+)-2,2’epicytoskyrin a on male mice (Mus musculus)

Oral subchronic toxicity of (+)-2,2’epicytoskyrin a on male mice (Mus musculus)

Quality Profile of Smoked African Sharptooth Catfish (Clarias Gariepinus) Treated With Uncaria Gambier (Uncaria Gambir Roxb)

Optimization of Bis-anthraquinones Production from Endophytic Fungi Diaporthe sp. GNBP-10

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