Antibacterial activity of Lactobacillus spp. isolated from the feces of healthy infants against enteropathogenic bacteria

Davoodabadi, Abolfazl; Dallal, Mohammad Mehdi Soltan; Foroushani, Abbas Rahimi; Douraghi, Masoumeh; Yazdi, Mohammad Kazem Sharifi; Harati, Farzaneh Amin

doi:10.1016/j.anaerobe.2015.04.014

Cited by 55 publications

(41 citation statements)

References 28 publications

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“…In this study, L. fermentum isolates (FH5, FH13 and FH18) had greater adhesion to Caco‐2 cells than did other Lactobacillus strains. This result is similar to the finding of Davoodabadi et al ., who found that L. fermentum 40‐2 and L. fermentum 40‐3 had more capacity to adhere to epithelial cells than did other isolates .…”

Section: Discussionmentioning

confidence: 97%

“…Also, some probiotic properties such as gut survival, adhesion to epithelium cells and inhibition of pathogen bacteria have been identified in lactobacilli isolated from humans . Review of published reports yielded little information about Lactobacillus species isolated from infant feces in Iran . In contrast to a previous study, in the current study the antibacterial activity of isolates against either gram‐positive or ‐negative bacteria was assessed by spot tests and disc diffusion methods.…”

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confidence: 88%

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Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

Halimi

Mirsalehian

2016

Microbiology and Immunology

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The probiotic potential of Lactobacillus species isolated from infant feces was investigated. For this study, the antibiotic susceptibility, tolerance in gut-related conditions, antimicrobial activity, and ability to adhere to a human colorectal adenocarcinoma cell line (Caco-2 cells) of four commonwere assessed. Most isolates that which were sensitive to imipenem, ampicillin, gentamycin, erythromycin and tetracycline were selected for other tests. L. gasseri isolates had the greatest sensitivity to gastric and intestinal fluids (<10% viability). L. fermentum (FH5, FH13 and FH18) had the highest adhesion to Caco-2 cells. The lowest antibacterial activity against pathogenic bacteria was shown by L. gasseri strains in spot tests. Furthermore, non-adjusted cell-free culture supernatants with low pH had greater antimicrobial activity, which was related to organic acid. The results showed that some isolates of L. rhamnosus and L. fermentum are suitable for use as a probiotic.

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Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 88%

Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

Halimi

Mirsalehian

2016

Microbiology and Immunology

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“…They are widely found in various ecosystems, especially in foods such as dairy products, fermented vegetables, silage, or fermented beverages. Lactobacilli are one of the most prevalent groups of human-associated bacteria, being a part of the gastrointestinal, respiratory, and genital tracts of humans [5,10].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains

Krausová¹,

Hyršlová²,

Hynstova³

2019

Fermentation

155

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We isolated several strains of human and animal origin, focusing on the genera Lactobacillus and Bifidobacterium. Samples of cow colostrum, feces and saliva of calves and piglets, and infant feces were acquired, bacterial strains were isolated and identified, and twenty strains of lactobacilli and bifidobacteria were shortlisted for further in vitro analysis of adhesion capacity to human cells, surface hydrophobicity, and auto-aggregation. Adhesion capacity was evaluated using a mixture of Caco-2 and HT-29-MTX cells and hydrophobicity was measured using the microbial adhesion to the hydrocarbon method. Lactobacillus reuteri was the most frequently isolated species, followed by L. casei subsp. paracasei and L. gasseri; all strains were isolated from infant feces. Bifidobacteria were represented by the species B. longum (infant feces), and B. thermophilum and B. pseudolongum (calves, piglets). All twenty strains showed good adhesion capacities to the mixed cell culture (17.7–37.2%), particularly, L. reuteri isolates K7 and K14 (37.2% and 35.5%, respectively). In hydrophobicity and auto-aggregation assays, strain-specific differences irrespective of the origin or taxonomic group were observed. Hydrophobicity values varied considerably (from 6.1% to 87.4%), whereas auto-aggregation ability ranged from 21.7% to 69.7%. No relation was observed between hydrophobicity and adhesion capacity; instead, auto-aggregation was apparently related with adhesion.

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“…Identification of these two strains was performed with 16S rDNA gene sequencing. In brief, genomic DNA was extracted according to a previously described method [8]. The PCR primer sequences were as follows: forward primer 5′-CTCGTTGCGGGACTTAA-3′, and reverse primer 5′-GCAGCAGTAGGGAATCTTC-3′ (Bioneer, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…live microorganisms as a food supplement to benefit health, such as exerting an antagonistic effect on the gastroenteric pathogens Clostridium difficile, Campylobacter jejuni, Helicobacter pylori and rotavirus, neutralizing food mutagens produced in the colon and lowering serum cholesterol [7]. Various health and nutritional effects of lactic acid bacteria have been described, including improvement of the quality of human and animal foods, metabolic stimulation of the synthesis of vitamins and enzymes, stabilization of the intestinal microflora, competence with intestinal pathogens, host innate immune boost, production of antimicrobials, reduction of the risk of colon cancer by neutralizing carcinogens and suppression of tumors by modulating the probiotic strains [8], [9]. Probiotic properties are not seen in all strains but rather are seen only in certain species, depending on the strain [10].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of Lactobacillus plantarum and Lb. fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections

Dallal

Davoodabadi

Abdi

et al. 2017

New Microbes and New Infections

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Nosocomial infection constitutes a major public health problem worldwide. Increasing antibiotic resistance of pathogens associated with nosocomial infections has also become a major therapeutic challenge for physicians. Thus, development of alternative treatment protocols, such as the use of probiotics, matters. The aim of this research was to determine the antagonistic properties of Lactobacillus plantarum and Lb. fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections. One hundred five samples of nosocomial infections were collected and processed for bacterial isolation and antimicrobial susceptibility testing following standard bacteriologic techniques. The antibiotic susceptibility test was performed by the disk diffusion method, and antagonistic effect of Lactobacillus strains was investigated by well diffusion method. Of 105 samples, a total of 29 bacterial strains were identified as nonfermentative bacteria, including 17 Acinetobacter baumannii and 12 Pseudomonas aeruginosa. A. baumannii showed high resistance to tested antibiotics except ampicillin/sulbactam, and P. aeruginosa showed resistance to ampicillin/sulbactam and gentamicin and sensitive to amikacin and meropenem. Lb. plantarum had antagonistic properties against both A. baumannii and P. aeruginosa strains. Lb. plantarum had considerable effects on preventing the growth of A. baumannii and P. aeruginosa strains. However, further research is needed to better understanding of these effects on P. aeruginosa.

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Antibacterial activity of Lactobacillus spp. isolated from the feces of healthy infants against enteropathogenic bacteria

Cited by 55 publications

References 28 publications

Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains

Inhibitory effect of Lactobacillus plantarum and Lb. fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections

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