Antimicrobial effect of bioceramic cements on multispecies microcosm biofilm: a confocal laser microscopy study

Jardine, Alexander Pompermayer; Montagner, Francisco; Quintana, Ramiro Martins; Zaccara, Ivana Maria; Kopper, Patrícia Maria Poli

doi:10.1007/s00784-018-2551-6

Cited by 30 publications

(21 citation statements)

References 38 publications

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“…29 Pompermayer et al found that MTA was not effective against multispecies microcosm biofilms including E. faecalis, Streptococcus salivarius (S. salivarius), Streptococcus mutans (S. mutans) and so on. 30 In this study, the E. faecalis biofilms treated with MTA was still intact, and the proportion of live bacteria was high, suggesting that MTA had no obvious antibacterial effect on E. faecalis biofilms, which was similar to previous studies. MCSNs has antibacterial effect on planktonic E. faecalis, but the antibacterial effect of MCSNs on E. faecalis biofilms is obviously lower than that of CH.…”

Section: Discussionsupporting

confidence: 90%

<p>The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles</p>

Leng¹,

Li²,

Zhu³

et al. 2020

IJN

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Background: Mesoporous calcium-silicate nanoparticles (MCSNs) have good prospects in the medical field due to their great physicochemical characteristics, antibacterial activity and drug delivery capacity. This study was to analyze the antibiofilm activity and mechanisms of silver (Ag) and zinc (Zn) incorporated MCSNs (Ag/Zn-MCSNs) with different percentages of Ag and Zn. Methods: Ag/Zn(1:9, molar ratio)-MCSNs and Ag/Zn(9:1, molar ratio)-MCSNs were prepared and characterized. Endocytosis of nanoparticles by Enterococcus faecalis (E. faecalis) treated with Ag/Zn-MCSNs was observed using TEM to explore the antibacterial mechanisms. The antibiofilm activity of Ag/Zn-MCSNs with different ratios of Ag and Zn was tested by E. faecalis biofilm model in human roots. The human roots pretreated by different Ag/Zn-MCSNs were cultured with E. faecalis. Then, SEM and CLSM were used to observe the survival of E. faecalis on the root canal wall. Cytotoxicity of the nanoparticles was tested by CCK8 kits. Results: The Ag/Zn-MCSNs release Ag + and destroy the cell membranes to kill bacteria. The MCSNs containing Ag showed antibacterial activity against E. faecalis biofilms in different degrees, and they can adhere to dentin surfaces to get a continuous antibacterial effect. However, MTA, MCSNs and Zn-MCSNs could not disrupt the bacterial biofilms obviously. MCSNs, Ag/Zn(1:1, molar ratio)-MCSNs and Ag/Zn(1:9)-MCSNs showed no obvious cytotoxicity, while Ag-MCSNs and Ag/Zn(9:1)-MCSNs showed cytotoxicity. Zn-MCSNs can slightly promote cell proliferation. Conclusion: Ag/Zn-MCSNs have good antibiofilm activity. They might achieve an appropriate balance between the antibacterial activity and cytotoxicity by adjusting the ratio of Ag and Zn. Ag/Zn-MCSNs are expected to be a new type of root canal disinfectant or sealer for root canal treatment.

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Section: Discussionsupporting

confidence: 90%

<p>The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles</p>

Leng¹,

Li²,

Zhu³

et al. 2020

IJN

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“…Regarding the antimicrobial activity of the tested materials, neither formulation satisfactorily inhibited bacterial growth in comparison to direct contact with chlorhexidine 2% in both methods. Previous studies showed similar results for ProRoot MTA [30] and Biodentine [31] using other methods. Solubility halos were observed over agar, showing that this initial test seems to be inadequate due to possible misleading interpretation.…”

Section: Discussionsupporting

confidence: 66%

Physicochemical, antimicrobial, and biological properties of White-MTAFlow

et al. 2020

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Where a licence is displayed above, please note the terms and conditions of the licence govern your use of this document.When citing, please reference the published version. Take down policyWhile the University of Birmingham exercises care and attention in making items available there are rare occasions when an item has been uploaded in error or has been deemed to be commercially or otherwise sensitive.

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“…However, the results of the present study indicate that the type of repair material did have an effect on the viability of the colonizing bacteria, with significantly more live bacteria detected in the MTA-Angelus group than in the Endocem and Biodentine groups. This is in contrast to a previous study by Jardine et al (2019) who used confocal laser microscopy to evaluate the viability of a multispecies microcosm in the vicinity of bioceramic cements and concluded that MTA-Angelus was as effective as Biodentine in terms of antimicrobial activity [51]. This discrepancy may be explained by the different microorganisms involved.…”

Section: Discussioncontrasting

confidence: 79%

Bacterial Colonization and Proliferation in Furcal Perforations Repaired by Different Materials: A Confocal Laser Scanning Microscopy Study

et al. 2021

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Following furcal perforation, bacteria may colonize the defect and cause inflammation and periodontal destruction. This study used confocal laser scanning microscopy (CLSM) to evaluate Enterococcus faecalis colonization and proliferation in furcal perforations repaired with different materials. Furcal perforations created in 55 extracted human mandibular molars were repaired using either MTA-Angelus, Endocem, or Biodentine and coronally subjected to E. faecalis suspension for 21 days. The specimens were then stained using a LIVE/DEAD Viability Kit and visualized by CLSM. The minimum and maximum depths of bacterial penetration into the dentinal tubules were 159 and 1790 μM, respectively, with a mean of 713 μM. There were significantly more live than dead bacteria inside the dentinal tubules (p = 0.0023) in all groups, and all three repair materials exhibited a similarly sized stained area (p = 0.083). However, there were significant differences in the numbers of dead bacteria at the circumference of the perforation defect (p = 0.0041), with a significantly higher ratio of live to dead bacteria in the MTA-Angelus group (p = 0.001). Following perforation repair, bacteria may colonize the interface between the repair material and dentin and may penetrate through the dentinal tubules. The type of repair material has a significant effect on the viability of the colonizing bacteria.

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Antimicrobial effect of bioceramic cements on multispecies microcosm biofilm: a confocal laser microscopy study

Cited by 30 publications

References 38 publications

<p>The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles</p>

<p>The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles</p>

Physicochemical, antimicrobial, and biological properties of White-MTAFlow

Bacterial Colonization and Proliferation in Furcal Perforations Repaired by Different Materials: A Confocal Laser Scanning Microscopy Study

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