Antineuroinflammation activity of n-butanol fraction of Marsilea crenata Presl. in microglia HMC3 cell line

Abstract

BackgroundNeuroinflammation is one of the main causes of neurodegenerative events. Phytoestrogen is a group compounds that have an estrogen-like structure or function. Phytoestrogen has a high potential to overcome neuroinflammation caused by estrogen deficiency in postmenopausal women. Marsilea crenata Presl. is a plant known to contain phytoestrogens. This research aimed to analyze the activity of an n-butanol fraction of M. crenata leaves in inhibiting the classical pathway activation of microglia HMC3 cell line to M1 polarity, which has proinflammatory characteristics.MethodsMicroglia HMC3 cell line was cultured in Eagle’s minimum essential medium and induced with IFN-γ for 24 h to activate the cell to M1 polarity in 24-well microplates. The n-butanol fraction was added with various doses of 62.5, 125, and 250 ppm and genistein 50 μM as a positive control. The expression of major histocompatibility complex II (MHC II) as a marker was tested using a confocal laser scanning microscope.ResultsThe result of MHC II measurement shows a significant difference in the MHC II expression in the microglia HMC3 cell line between the negative control and all treatment groups at p<0.05, indicating a non-monotonic dose-response profile.ConclusionsThe best dosage to inhibit MHC II expression was 250 ppm with the value of 200.983 AU. It is then concluded that n-butanol fraction of M. crenata leaves has antineuroinflammation activity due to its phytoestrogens.

Marsilea crenata ethanol extract prevents monosodium glutamate adverse effects on the serum levels of reproductive hormones, sperm quality, and testis histology in male rats

“…The leaves of this plant are commonly used as vegetables in Surabaya, Indonesia. The pharmacological activity of the plant includes antineuroinflammation [ 19 ], anticholesterol [ 20 ], antiosteoporotic [ 21 ], and skin aging treatment [ 22 ]. The pharmacological studies of M. crenata remain limited compared with other species in the Marsileaceae family (e.g., Marsilea minuta Linn).…”

In VITRO ANTI-NEUROINFLAMMATORY EFFECT OF GENISTEIN (4',5,7-Trihydroxyisoflavone) ON MICROGLIA HMC3 CELL LINE, AND IN SILICO EVALUATION OF ITS INTERACTION WITH ESTROGEN RECEPTOR-β

“…Preparation of cell culture was conducted according to a procedure developed by Central Laboratory of Life Sciences, Universitas Brawijaya, Indonesia [18][19][20]. Microglia HMC3 cell line was cultured in a 25 cm 2 flask containing±250.000 cells with a complete medium containing 5 ml EMEM, 10% FBS, and 1% penicillin-streptomycin.…”

Analisis Kandungan Logam Berat Cd, Hg, dan Pb Daun Semanggi (Marsilea crenata Presl.) di Desa Semen, Kecamatan Pagu, Kabupaten Kediri

“…Fraksi dari ekstrak n-heksan M. crenata dapat meningkatkan pembentukan tulang melalui induksi MC3T3-E1 sel osteoblast dalam proses diferensiasi dikarenakan aktivitas ALP (alkaline phosphatase) [3]. Ekstrak etanol 96% ethanol dan fraksi n-butanol dari M. crenata juga memiliki aktivitas antineuroinflamasi, salah satu penyebab neuroinflamasi disebabkan oleh defisiensi estrogen pada wanita menopause [4] [5]. Pada simulasi molecular docking senyawa dari ekstrak etanol 96% M. crenata terdapat tiga senyawa yang dipredisi memiliki aktivitas anti-infalmasi yang sama dengan 17β-estradiol, senyawa tersebut merupakan prochlorperazine, 12-Aminododecanoic acid, and 1-methyl-2-[(4-methylpiperazin1yl)methyl]benzimidaol-5-amine hydrochloride [6].…”