Association of DNA Aneuploidy with Human Papillomavirus‐Induced Malignant Transformation of Sinonasal Transitional Papillomas

Abstract

The nuclear DNA content of 19 transitional papillomas of the sinonasal region and 9 maxillary squamous cell carcinomas was studied by flow cytometry; the presence of human papillomavirus (HPV) DNA types 11 and 16 was determined by the in situ hybridization technique from paraffin-embedded tissue. Thirteen (68%) of the papillomas and none of the carcinomas contained HPV genome. Six (32%) of the papillomas and 4 (44%) of the carcinomas had an aneuploid DNA content. The relative DNA content (DNA index) of the aneuploid maxillary carcinomas was larger than that of aneuploid papillomas (p = 0.004). Three of the papillomas underwent malignant transformation, all three of which contained HPV type 16 DNA; two were also aneuploid. Data indicate that papillomas containing HPV type 16 DNA have a tendency (p = 0.06) to undergo malignant transformation, and that this tendency is greater if DNA aneuploidy or HPV type 11 DNA is also present (p = 0.02).

A high prevalence of human papillomavirus DNA in recurrent nasal papillomas

“…Although all the 12 nasal papillomas examined in this study had no evidence of cancerous change, the tendency for malignant transformation of nasal papillomas [ 11, and especially nasal papillomas with HPV 16 DNA [7], has been reported. Therefore, careful follow-up of patients with nasal papillomas containing HPV 16 DNA would seem to be important.…”

“…Among them, all of seven recurrent cases contained HPV DNA. Klemi et al [7] also reported that all four cases of recurrent sinonasal papillomas yielded HPV DNA types 11/16, and Fu et al [12] reported all three recurrent fungiform nasal papillomas studied had HPV DNA types 6/11. Conversely, HPV was not detected in normal nasal mucosa [13].…”

“…Among them, four (SO.OO/) of the five recurrent cases (cases 1-5) were HPV DNA-positive whereas only one (14.3%) of seven non-recurrent cases (cases [6][7][8][9][10][11][12] was HPV DNA-positive. The detection rate of HPV DNA in recurrent cases analysed by Fisher's exact probability test was significantly higher than that in non-recurrent cases (p = 0.045).…”

“…A clinical tendency for the lesion to recur even after adequate removal suggests the possibility of papillomas being induced by HPV Laryngeal papillomas are classified clinically into four groups: juvenile multiple, juvenile single, adult multiple and adult single [22]. HPV type 6/11 has been detected in almost 100% of juvenile and adult multiple laryngeal papillomas, strongly suggesting a viral aetiology for these benign tumors [23-251. Conversely, the reported prevalence of HPV in fungiform exophytic and inverted nasal papillomas has varied [l, [3][4][5][6][7][8][9][10][11][12][13]151. The methods and techniques used for detection of HPV as well as the patients' geographic location seem to influence the results.…”

Schneiderian Papillomas and Nonsalivary Glandular Neoplasms of the Head and Neck

“…There is some evidence, however, to suggest that HPV 16 and 18 may be more carcinogenic than HPV 6 and 11 (45,46,49). In the studies of Klemi et al (45) (32%) were aneuploid, and of the 6 that were aneuploid, 2 (33%) were associated with carcinomas.…”