Biological Oxidase Enzyme Mimetic Cu‐Pt Nanoalloys: A Multifunctional Nanozyme for Colorimetric Detection of Ascorbic Acid and Identification of Mammalian Cells

Abstract

Nanomaterials serving as artificial enzymes of the new generation (nanozymes), exhibit enhanced catalytic performance, excellent stability, low cost of production, and other associated properties of nanoparticles. This emerging field of nanozymes has broad-spectrum potential applications in the bridging area nanotechnology and biomedicine. Among metal-based nanozymes, bimetallic nanoparticles have been found to show enhanced catalytic performance and display ability to catalyze multistep reactions typically not observed in their monometallic counterparts. Herein, we report the synthesis of CuÀ Pt based bimetallic nanoalloys coated with a nonionic surfactant polyoxyethylene cholesteryl ether. The characterization data sug-gest that the developed nanoalloys have an almost uniform distribution of Cu and Pt (1:1) elements; therefore, this novel technique could be used for the large-scale synthesis of CuÀ Pt based nanozymes in a one-step procedure. Further investigations showed that these bimetallic nanoalloys possess excellent biological oxidase enzyme-like activity and the catalytic activity could be performed at harsh conditions of pH and temperature. Oxidase activity of CuÀ Pt nanoalloys was utilized for the sensitive detection of ascorbic acid in the sub-micromolar concentration range. Supplementing ATP in the oxidase reaction, the nanozyme showed enhanced catalytic activity, which was used for the detection of human liver cells.

Platinum–Copper Bimetallic Colloid Nanoparticle Cluster Nanozymes with Multiple Enzyme-like Activities for Scavenging Reactive Oxygen Species

“…As a versatile bimetallic catalyst, Pt–Cu bimetallic nanomaterials have attracted much interest in the catalysis field since they display more superior catalytic performance and stability than individual Pt nanomaterials. − Thus, the introduction of copper in the Pt-based nanozyme has the potential to improve its catalytic efficiency and versatility due to the synergistic effect of the two components. In this work, PVP–platinum–copper nanoparticle clusters (PVP-PtCuNCs) were synthesized by a simple one-step method based on PVP.…”

In situ generated Fe3C embedded Fe–N-doped carbon nanozymes with enhanced oxidase mimic activity for total antioxidant capacity assessment

“…Compared with natural enzymes, nanozymes offered many distinct advantages, such as low cost, ease of large-scale preparation, tolerance to harsh environments and high operational stability. [1][2][3][4][5][6] To date, a myriad of nanomaterials with enzyme-mimicking properties have been identified, including metal oxides, 7,8 metal sulfides, 9 noble metal materials, 10 bimetallic alloy materials, 11,12 metal-organic frameworks, 13,14 Prussian blue 15 and carbon-based materials. 6 Among them, carbon-based materials are one of the research hotspots due to their good biocompatibility and well-defined electronic and geometric structures.…”

Nitrogen, phosphorus co-doped hollow porous carbon microspheres as an oxidase-like electrochemical sensor for baicalin

“…40 Several nanomaterials, including metal nanoparticles, metal oxides, and carbon nanomaterials, have been found to possess enzymelike properties. These nanozymes with enzyme-like characteristics coupled with different instrumental analytical methods, such as colorimetry, 41,42 fluorometry, 43 and electrochemistry, 44 have been developed and applied in bioanalysis, environmental analysis and medical analysis. 45 At present, the research on nanozymes mostly focuses on colorimetric sensing, while the enzyme-like characteristics of nanomaterials in electrochemical processes have received less attention.…”