Bone tissue response to <scp>BMP</scp>‐2 adsorbed on amorphous microporous silica implants

Chaudhari, Amol; Cardoso, Marcio Vivan; Martens, Johan A.; Vandamme, Katleen; Naert, Ignace; Duyck, Joke

doi:10.1111/jcpe.12005

Cited by 8 publications

(6 citation statements)

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“…Additional BMP-2 does not allow stem cells to maturate into osteogenic cells, leaving them in a pre-osteogenic stage. Based on that, we can conclude that BMP-2 fails to stimulate bone regeneration and osseointegration as also shown by Chaudhari A. et al [ 37 ].…”

Section: Discussionsupporting

confidence: 77%

Dental follicle stem cells in bone regeneration on titanium implants

et al. 2015

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BackgroundWe aimed to demonstrate that DF stem cells from impacted molars and canines can be used to improve bone regeneration on titanium implants surfaces. This study highlights the presence of stem cells in DF, their potential to adhere and differentiate into osteoblasts on different types of titanium surfaces.ResultsIsolated cells from the harvested DF tissue from impacted canine/molars, expressed stem cells markers. Differentiation into bone cells was induced in presence or absence of BMP-2 and TGFβ1. The presence of growth factors until 28 days in medium maintained the cells in an earlier stage of differentiation with a lower level of specific bone proteins and a higher expression of alkaline phosphatase (ALP). Influence of titanium implants with different bioactive coatings, hydroxyapatite (TiHA) and with silicatitanate (TiSiO2), and porous Ti6Al7Nb implants as control (TiCtrl), was studied in terms of cell adhesion and viability. Ti HA implants proved to be more favorable for adhesion and proliferation of DF stem cells in first days of cultivation. The influence of titanium coatings and osteogenic differentiation mediums with or without growth factors were evaluated. Additional BMP-2 in the medium did not allow DF stem cells to develop a more mature phenotype, leaving them in a pre-osteogenic stage. The best sustained mineralization process evaluated by immuno-cytochemical staining, scanning electron microscopy and Ca2+ quantification was observed for TiHA implants with a higher expression of ALP, collagen and Ca2+ deposition. Long term culturing (70 days) on titanium surfaces of DF stem cells in standard medium without soluble osteogenic inducers, indicated that HA coating is more favorable, with the acquisition of a more mature osteoblastic phenotype as shown by immunocytochemical staining. These findings demonstrated that even in absence of exogenous osteogenic factors, TiHA implants and in a lesser extent TiCtrl and TiSiO2 implants can induce and sustain osteogenic differentiation of DF stem cells, by their chemical and topographical properties.ConclusionsOur research demonstrated that DF stem cells have a spontaneous tendency for osteogenic differentiation and can be used for improving bone regeneration on titanium implants surfaces.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-015-0229-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 77%

Dental follicle stem cells in bone regeneration on titanium implants

et al. 2015

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“…antibiotic-impregnated cements, pastes, powders, degradable sponges or fleecesare used in orthopedic surgery [7]. Nevertheless, only few nanomaterials can both easily be osseointegrated and slowly release antibiotic or anti-inflammatory drugs [5,[8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Adsorption and release of ampicillin antibiotic from ordered mesoporous silica

Nairi

Medda

Monduzzi

et al. 2017

Journal of Colloid and Interface Science

127

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“…However, it has also been reported that BMP can exert a negative effect on bone formation (Wikesjö et al, 2008) as it may play a role in osteoclastogenesis as receptors for BMPs are also expressed in osteoclasts (Kaneko et al, 2000). Although osteoclast activity is inherent in the process of bone healing around implants, large doses of BMP-2 may lead to extensive bone re-modelling with consequent and undesirable bone loss (Chaudhari et al, 2012;Wikesjö et al, 2008). Unfortunately, only limited information is available regarding bone resorption due to a particular BMP-2 release via protein delivery carriers (Chaudhari et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…A pig animal model was used to simulate the osseointegration in its full complexity (Cardoso et al., ; Chaudhari et al., ). Domestic pigs show considerable bone similarities to human beings, including important aspects such as osseous macrostructure, microstructure, composition and bone remodelling process and rate, therefore representing a suitable option for studies involving implants (Pearce et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Although osteoclast activity is inherent in the process of bone healing around implants, large doses of BMP‐2 may lead to extensive bone re‐modelling with consequent and undesirable bone loss (Chaudhari et al., ; Wikesjö et al., ). Unfortunately, only limited information is available regarding bone resorption due to a particular BMP‐2 release via protein delivery carriers (Chaudhari et al., ). Additional studies are necessary to investigate the most adequate doses of BPM‐2 to be adsorbed onto PPL‐functionalized implants.…”

Section: Discussionmentioning

confidence: 99%

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