Characterisation of Extended Spectrum Beta-Lactamase Producing E. coli from Secondary and Tertiary Hospitals in South Eastern Nigeria

Ifeanyichukwu, Iroha; Adikwu, M. U.; Amadi, ES; Aibinu, I.; Esimone, CO

doi:10.3923/jm.2008.514.519

Cited by 18 publications

(19 citation statements)

References 9 publications

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“…In South and Eastern Nigeria, prevalence of ESBL among E. coli isolated from pregnant patients from 2003-2007 was very high (52.4%) (Aibinu et al, 2003;Iroha et al, 2008) and is at variance with the findings of the present study. This may likely due to geographical location and antimicrobial usage.…”

Section: Discussioncontrasting

confidence: 99%

“…Present study reveals 26.1% prevalence of ESBL-enzyme production among UPEC in symptomatic UTIs pregnant women in Ondo and Ekiti States, Nigeria. This contradicts the earlier reports of 75% by Padmavathy et al (2014), 52.4% by Yusuf et al (2005), 52.4% by Iroha et al (2008), and 37.3% by Aibinu et al (2003) on prevalence of ESBL enzymes detected in E. coli from UTI patients in the East, South and Northern Nigeria, respectively. Prevalence of ESBL-producing UPEC in this study is higher than reports from other countries and other parts of Nigeria.…”

Section: Discussioncontrasting

confidence: 84%

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Extended Spectrum Beta- Lactamase- Producing Uropathogenic Escherichia coli in Pregnant Women Diagnosed With Urinary Tract Infections in South-Western Nigeria

Olufunke¹,

Abiodun²,

Dunah³

2014

JMBR

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Extended spectrum beta-lactamase (ESBL)-producing uropathogenic Escherichia coli (UPEC) in symptomatic pregnant women with confirmed urinary tract infections in Southwest. Nigeria was reported. Susceptibility of UPEC isolates to β-lactam and other classes of antibiotics was determined by the Kirby-Bauer's disc diffusion method on Mueller-Hinton agar plate. Detection of plasmid DNA in multiple antibiotic resistant isolates was carried out by alkali lysis (TENS) method. Extended-spectrum-β-lactamase (ESBL) production was determined by double disk synergy test (DDST). Isolates that were positive to ESBL were mated with non-ESBL-producing E. coli and other enterics in a conjugation experiment. Transfer of ESBL-enzyme and other resistance phenotypes in the transconjugants was investigated by DDST. Data obtained were statistically analyzed using SPSS 17. Greater percentage of the isolates were multiple antibiotic resistant (MAR). Sixty-nine (26.1 %) of UPEC were ESBL producers. Some of the ESBL producers transferred ESBL-enzyme and other resistance determinants to the recipients. Large size plasmid DNA of molecular weight (23.13-33.04 kb) was detected in some representative MAR isolates.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 84%

Extended Spectrum Beta- Lactamase- Producing Uropathogenic Escherichia coli in Pregnant Women Diagnosed With Urinary Tract Infections in South-Western Nigeria

Olufunke¹,

Abiodun²,

Dunah³

2014

JMBR

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“…The result of the study revealed that 38 (36.2%) isolates were positive for ESBL production 15 (34.9%) isolates were from urine, 10 (26.3%) from stool and 13 (54.2%) from blood respectively (Iroha et al, 2008). The study was aimed at determining the rate occurrence of ESBLs among enterobacteriaceae isolates in the study site via isolation of enterobacteriaceae from clinical specimens and detection of ESBLs among the isolates.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of extended spectrum β-lactamases (esbls) among enterobacteriaceae in murtala mohammed specialist hospital, Kano, Nigeria

Yusha’u¹,

Aliyu²,

Kumurya³

et al. 2010

Bayero J. Pure App. Sci.

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Confirmed variants of enterobacteriaceae isolated from 143 patients that attended MurtalaMohammed Specialist Hospital Kano, were screened for extended spectrum β-lactamases (ESBLs) production using Clinical Laboratory Standards Institute (CLSI) breakpoint. Suspected ESBLs producers were subjected to confirmation using Disc Replacement Method (DRM). Standard discs of augmentin {AMC 30μg (Oxoid, England)}, ceftriaxone {AUF 30μg (Oxoid, England)} and ceftazidime {RP 30μg (Oxoid, England)} were used in the screening. Of the 143 isolates screened, 114 (79.72%) were Gram negative isolates belonging to the family enterobacteriaceae. Among the enterobacteriaceae isolates screened, the results of CLSI breakpoint test showed that 76 (66.7%) were ESBLs producers viz: Citrobacter spp.

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“…16 In the available literature, there are numerous studies [17][18][19] on the occurrence and antibiogram of ESC-/ FOX-resistant and ESBL-/AmpC-P E. coli in hospitalized (in-and out-) patients and the community, including Nigeria. [20][21][22][23] However, reports on the colonization of healthy asymptomatic individuals by these organisms are rather scanty. [17][18][19] Studies which assessed the occurrence of ESC-resistant/ESBL-/AmpC-P E. coli in asymptomatic healthy university students included that of Kader et al in Saudi Arabia, 24 Chirindze et al in Mozambique, 25 Li et al in China 26 and Lonchel et al in Cameroun.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Antibiogram of Extended-Spectrum Cephalosporin- and Cephamycin-Resistant Escherichia coli in Asymptomatic University Students

Anyanwu

Okpalanwa

Ugwuanyi

2019

Int J Enteric Pathog

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Background: Apparently healthy individuals could serve as reservoirs and disseminators of extendedspectrum cephalosporin (ESC)- and cephamycin (cefoxitin, FOX)-resistant, and extended-spectrum β-lactamase-producing (ESBL-P) Escherichia coli which jeopardizes antibacterial therapy thereby posing a threat to the health of infected individuals/carriers. Objectives: This study aimed to screen healthy asymptomatic students in the University of Nigeria, Nsukka (UNN) as potential reservoirs of ESC- and FOX-resistant and ESBL-P E. coli and to determine the antibacterial resistance profile. Materials and Methods: Anal swabs were collected from 190 randomly selected healthy asymptomatic students of both genders in UNN between March and July 2018. ESC-resistant E. coli was isolated using MacConkey agar with 2 µg/mL ceftazidime. ESBL production was assessed by combination disc method while cephamycin resistance was determined using cefoxitin disc screening. Phenotypic resistance of the isolates was determined using disc diffusion method. Results: Out of 190 samples, 20 (10.2%) demonstrated growth. Of these, 6 (30%) were FOX resistant (putative AmpC-producers) but none produced ESBL. The resistance of the isolates was 100% to ampicillin (AMP), 95% to ceftazidime (CAZ), tetracycline (TET) and sulfamethoxazole-trimethoprim (SXT), 30% to FOX and chloramphenicol (CHL), 85% to ciprofloxacin (CIP), enrofloxacin (ENR) and streptomycin (STR), and 65% to kanamycin (KAN). All the isolates were susceptible to meropenem (MEM). Among the 20 isolates, 1 (5%) was resistant to 2 classes of antibacterial agents while 19 (95%), including all the FOX-resistant strains, were resistant to ≥ 3 classes of antibacterial agents. The isolates exhibited 11 multiple antibacterial resistance patterns with AMP, CAZ, FOX, TET, CIP, ENR, STR, KAN, SXT being predominant. Conclusion: Healthy asymptomatic students in UNN are potential reservoirs and disseminators of ESC- and cephamycin (FOX)-resistant E. coli.

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Characterisation of Extended Spectrum Beta-Lactamase Producing E. coli from Secondary and Tertiary Hospitals in South Eastern Nigeria

Cited by 18 publications

References 9 publications

Extended Spectrum Beta- Lactamase- Producing Uropathogenic Escherichia coli in Pregnant Women Diagnosed With Urinary Tract Infections in South-Western Nigeria

Extended Spectrum Beta- Lactamase- Producing Uropathogenic Escherichia coli in Pregnant Women Diagnosed With Urinary Tract Infections in South-Western Nigeria

Prevalence of extended spectrum β-lactamases (esbls) among enterobacteriaceae in murtala mohammed specialist hospital, Kano, Nigeria

Occurrence and Antibiogram of Extended-Spectrum Cephalosporin- and Cephamycin-Resistant Escherichia coli in Asymptomatic University Students

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