Characteristics of fresh and frozen semen of Red Chittagong Cattle

Abstract

Semen production from breeding bulls used in Artificial Insemination (AI) program is of great importance in cattle breeding. Good quality semen has positive effect on the fertility of cows and heifers. The aim of this study was to evaluate the quality and assessed as the freezing ability of Red Chittagong Cattle (RCC) bull with fresh-diluted semen and frozen semen. The parameters of semen characteristics included were volume per ejaculate (ml), percentage of mass motility, normal and abnormal sperm, and sperm concentration (million/ml). The highest mass motility was found in bull ID No. 4 and the lowest in bull ID No. 1 but the highest sperm concentration was found in bull ID No. 3 with lowest in bull ID No. 2 in fresh semen. Highly significant (p<0.001) positive correlation was found between mass motility and sperm concentration in bulls of ID No. 3 and 4, a negative significant (p<0.05) correlation was found between mass motility and normal sperm percentage. Two freezing techniques were followed in frozen semen production: Cork sheet freezing and Cryobath freezing with two freezing times 15 and 30 minutes were also applied. Variations in the motility of frozen semen by Cork sheet freezing with two different times were found significant (p<0.05) between bulls. Furthermore, highly significant (p<0.01) difference of motility by Cryobath freezing was found between the bulls. The highest motility after both 15 and 30 minutes Cork sheet freezing and Cryobath freezing was found in bull ID No. 4 and lowest in bull ID No. 3. In the present study, variability in the potentiality of bulls (semen volume and sperm concentration) was observed and found lower than crossbred bulls due to small size of Red Chittagong, an indigenous zebu cattle of Bangladesh. Bangladesh Journal of Animal Science. 51(1):25-30

Morphometric features, seminal profile and diluters effect on post-thaw semen quality of Munshiganj cattle in Bangladesh

“…Present study observation was comparatively lower than 9.3 ml [ 51 ], 6.3 ± 0.5 ml [ 52 ] in local bull. In contrary, this observation was comparatively higher than the report of 2.58–4.01 ml in RCC [ 53 ] and 3.22 ± 0.09 ml in RCC [ 54 ] that might be due to breed differences. Accessory gland secretions also play an important role in influencing ejaculate volume [ 55 ].…”

“…In the current study, average sperm concentration was found 1510.27 ± 844.07 million/ml which was similar with the findings in Munshiganj bull (1669.60 ± 192.07 million/ml) [ 48 ]and (1796 ± 122.29 million/ml) [ 56 ]. The present study findings was much higher than the report of [ 54 ] (907.39 ± 23.01 million/ml in RCC bull), and (1035.24 ± 279.79 to 1088.12 ± 297.14 million/ml) in Bali bull in Indonesia [ 49 ]. Breed may be the contributing factor for this difference.…”

Effect of Guava Filtrate Supplementation in Tris and Citrate-Based Extenders on Spermatozoa Quality of Brangus Bull after Sex-rest

“…The normal spermatozoa more than 80% was found in semen group III, more than 90% in group II and more than 95% in group I. This results was in accordance with reported by Arefin et al (2022) (Morrell et al, 2018).…”

“…The mass motility of spermatozoa in this study was in the average of 3 + (+++), which indicates the number of spermatozoa that were moving together very dense (densum), estimated more than 90% spermatozoa moving forward (progressive). This result followed by Arefin et al (2022), 62.11 ± 0,56% in Red Chittagong Cattle semen and Diansyah et al ( 2022), 94.94 ± 2.75%.…”

Comparative Study on Semen Quality and Fertility of Red Chittagong Cattle, BCB1 and Munshiganj Bulls of Bangladesh

“…Total, progressive, static and slow motility of the fresh vs. post thawed Munshiganj semen were (84.69 ± 4.28 vs. 52.97 ± 3.13), (72.53 ± 2.91 vs. 43.71 ± 1.57), (15.31 ± 4.28 vs. 47.03 ± 3.13) and (1.23 ± 0.60 vs. 0.58 ± 0.18) respectively [29]. The average mass motility of fresh and frozen sperm in RCC bulls ranged between 55.85% -64.31% and 44.50% -53.50% respectively [35]. Fresh sperm motility of Red Chittagong bulls was 62.12 ± 0.97% [27].…”