Combined Effect of a Microporous Layer and Type I Collagen Coating on a Biphasic Calcium Phosphate Scaffold for Bone Tissue Engineering

Abstract

In this study, type I collagen was coated onto unmodified and modified microporous biphasic calcium phosphate (BCP) scaffolds. Surface characterization using a scanning electron microscope (SEM) and a surface goniometer confirmed the modification of the BCP coating. The quantity of the collagen coating was investigated using Sirius Red staining, and quantitative assessment of the collagen coating showed no significant differences between the two groups. MG63 cells were used to evaluate cell proliferation and ALP activity on the modified BCP scaffolds. The modified microporous surfaces showed low contact angles and large surface areas, which enhanced cell spreading and proliferation. Coating of the BCP scaffolds with type I collagen led to enhanced cell-material interactions and improved MG63 functions, such as spreading, proliferation, and differentiation. The micropore/collagen-coated scaffold showed the highest rate of cell response. These results indicate that a combination of micropores and collagen enhances cellular function on bioengineered bone allograft tissue.

Biological properties of calcium phosphate biomaterials for bone repair: a review

“…Histology observations also conrmed that the sample covered with autogenous periosteum exhibited a remarkably higher osteogenesis rate and osteogenesis quality than the pure CaP substitute. 54 As one of the most abundant structural proteins in hard tissues and a well-known mediator of osteoblast cellular functions such as initial attachment, proliferation, and differentiation, 129 collagen has been widely explored as a coating on calcium phosphate scaffolds, including unmodied/modied microporous BCP 130 and HA. 129,131 Although these various coatings provide the scaffold with the unique enhancement of different properties such as improved osteoinductivity and adsorption of bone morphogenetic proteins, some coating solutions used for impregnating scaffolds may lead to the considerably decreased porosity of the scaffold, due to the high viscosity, which could impact the ingrowth of bone cells into the scaffold.…”

Enhanced Stability of Calcium Sulfate Scaffolds with 45S5 Bioglass for Bone Repair

“…Generally, the bone scaffold should be biocompatible to promote bone cell growth and scaffold-cell interactions, and it should be bioactive to bond strongly with bone tissues [5,6,7]. More importantly, the scaffold should possess proper stability in order to keep the shape in the process of bone repair [8].…”

Synthesis and Characterization of Biocomposite BCP/Collagen for Bone Material Scaffold

“…Ada banyak jenis kolagen yang terdapat pada hewan, salah satunya adalah kolagen tipe I. Kolagen tipe I, merupakan salah satu protein struktural yang banyak terdapat di jaringan keras, salah satunya adalah tulang (±38% dari komposisi tulang), dan merupakan mediator fungsi sel osteoblas (Lee et al 2015). Kolagen banyak diaplikasikan sebagai biomaterial karena mempunyai sifat biodegradasi, biokompotibel, dan dapat membantu pembentukan pori pada perancah (Chen et al 2012).…”