Comparison of DNA, aminoethylglycyl PNA and pyrrolidinyl PNA as probes for detection of DNA hybridization using surface plasmon resonance technique

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

“…Furthermore, no binding with the singlebase mismatched DNA target was observed for the PNAAu bioelectrode. Other groups further improved SPR measurements by introducing chemical modifications to PNA probes [73,74] and achieved better stability and reusability of the sensors. Likewise, dsDNA has been detected by use of a duplex invasion method [75], and localized SPR has been used by Endo et al [76] to detect 6.7×10 −13 mol L −1 ssDNA with base mismatch specificity.…”

Quaternized chitosan particles as ion exchange supports for label-free DNA detection using PNA probe and MALDI-TOF mass spectrometry

“…Recently, a conformationally restricted pyrrolidinyl PNA based on d-prolyl-2-aminocyclopentane-carboxylic acid backbones (acpcPNA) was introduced by Vilaivan and co-workers. This PNA system possessed higher binding affinity and sequence specificity toward DNA than that of Nielsen's PNA, and with a much stronger preference for the antiparallel binding mode (Ananthanawat, Vilaivan, Hoven, & Su, 2010;Suparpprom, Srisuwannaket, Sangvanich, & Vilaivan, 2005;Vilaivan & Srisuwannaket, 2006). The powerful discrimination for single mismatched DNA makes acpcPNA a potential candidate for the development of highly effective DNA biosensors, which has been continuously proven for both labeling (Boonlua, Vilaivan, Wagenknecht, & Vilaivan, 2011;Maneelun & Vilaivan, 2013;Mansawat, Boonlua, Siriwong, & Vilaivan, 2012;Rashatasakhon, Vongnam, Siripornnoppakhun, Vilaivan, & Sukwattanasinitt, 2012;Reenabthue, Boonlua, Vilaivan, Vilaivan, & Suparpprom, 2011) and non-labeling techniques (Ananthanawat, Hoven, Vilaivan, & Su, 2011;Ananthanawat, Vilaivan, Mekboonsonglarp, & Hoven, 2009;Jampasa et al, 2014;Sankoh et al, 2013;Theppaleak, Rutnakornpituk, Wichai, Vilaivan, & Rutnakornpituk, 2013a, 2013bThipmanee et al, 2012).…”

Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi

“…SPR imaging technology has proven to be an invaluable tool for high-throughput analysis of bio-molecular interactions (Kodoyianni et al, 2011) such as, drug discovery (Singh et al, 2014), biomarker screening (Shabani et al, 2013), nucleic acid detection (Roberta et al, 2013), food safety analysis (Piliarik et al, 2009), environmental analysis (Mauriz et al, 2007), DNA hybridization (Ananthanawat et al, 2010 andMalic et al, 2009), protein-DNA interaction (Pillet et al, 2013) and living cell activation (Hiragun et al, 2012). SPR sensing has been significantly developed in recent years to quantify biomarkers in complex matrixes, such as serum (Choi et al, 2010;Su et al, 2008 andLadd et al, 2009), plasma (Teramura et al, 2007), saliva (Yang et al, 2005) and cell lysate (Kyo et al, 2005).…”