In this review, the synthesis and biomedical application of Ag 2 S quantum dots(QDs) are comprehensively summarized with a particular focus on biological imaging and nanomedicine. For the first, the authors introduce the optical properties of Ag 2 S QDs, and then, the auhtors summarize a range of synthetic methods for Ag 2 S QDs. The subsequent section provides the applications of Ag 2 S QDs in optical imaging, photoacoustic imaging, sensing, optical tracking, and photothermal therapy. Finally, the authors provide insights into the overall situation, challenges, and future directions in this field.