Development of high oleic oil crop platform in flax through RNAi-mediated multiple FAD2 gene silencing

Chen, Yurong; Zhou, Xue-Rong; Zhang, Zhijun; Dribnenki, Paul; Singh, Surinder P.; Green, Allan

doi:10.1007/s00299-015-1737-5

Cited by 55 publications

(43 citation statements)

References 30 publications

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“…У арабидоп-сиса, как модельного объекта для изучения генетического контроля биосин-теза запасных липидов (Browse, Somerville, 1994, Ohlrogge, Browse, 1995, Chapman et al, 2012, было установлено, что изменение температуры вли-яет на проявление дефектных генов, контролирующих биосинтез ЖК, затрагивающее размеры растения, ан-тоциановую окраску и появление некрозов (Ohlrogge, Browse, 1995). У льна мутанты с нарушением биосин-теза ЖК получены только для генов fad2 и fad3, и у них не выявлено плейотропного эффекта на другие признаки (Vrinten et al, 2005;Thambugala et al, 2013;Chen et al, 2015).…”

Section: биохимическое разнообразие льна по жирнокислотному составу сunclassified

Biochemical diversity of fatty acid composition in flax from VIR genetic collection and effect of environment on its development

Porokhovinova

Шеленга

Косых

et al. 2016

Ecological genetics

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Background. In connection with climate change vary known patterns of environmental influences on the ratio of fatty acids(FA) in oil. Therefore,relevant data of modern geography test. Materials and methods. In work 24 lines and 3 commercial varieties of flax including 3 low linolenic (LL) accessions, grown in the Leningrad and Samara regions were used. FA composition was evaluated by gas chromatography for the ratio of palmitic (PAL), stearic (STE), oleic (OLE), linoleic (ω6,LIO), linolenic (ω3, LIN) acids, ω6/ω3 and iodine number of the oil (IOD). Results. The strongest differences are due to the level of LIN. It is lower in LL and gc-119 from India and higher in 3 lines carrying the gene s1 (deranged anthocyanin biosynthesis). In gc-119, contrast to LL, LIN decrease increase of OLE, instead of LIO. In lines with the gene s1 LIN increase due to the OLE reduction. Contrary to earlier publications the seeds of northern reproduction have more PAL, OLE, less LIN, IOD. 2F ANOVA revealed significant effect of genotype and reproductions place on PAL, OLE, LIN, IOD. LIO is affected only by genotype. Independence of ω6/ω3 is explained by strong abnormity of distribution due to LL. In high linolenic (HL) accessions group both factors influenced all characters except STE. Kruskal-Wallis H test (non-parametric 1F ANOVA analogue) show significant effect of genotype and place of reproductions on ω6/ω3. It reveals the impact of the reproduction place on LIN,no significant effect of genotype on OLE and IOD, which in the case of 1F ANOVA were significant. For characters of HL with normal distribution, comparing of both tests showed that in case of 0,01 p 0,09 conclusions concerning significance may vary,but in cases p 0,01 or p 0,10 they are identical. Conclusion. In our studies the geographical effect is less important than the weather in the year of growing. For abnormal distribution it is desirable to use both statistics and carefully make conclusions about the significance of differences in borderline probabilities.

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Section: биохимическое разнообразие льна по жирнокислотному составу сunclassified

Biochemical diversity of fatty acid composition in flax from VIR genetic collection and effect of environment on its development

Porokhovinova

Шеленга

Косых

et al. 2016

Ecological genetics

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“…Plant Breed., 51, 2015 (4): 123-141 doi: 10.17221/104/2015-CJGPB Ling and Binding (1997) 1999 genetic transformation of flax by particle bombardment Wijayanto and McHughen (1999) development of organ specific promoter from linseed for linseed transformation Jain et al (1999) first release of GM flax into environment in EU (flax lines after T-DNA insertional mutagenesis; randomly induced genetic/phenotype changes) Rakouský et al ( , 2001 Wróbel-Kwiatkowska et al (2007a) lignin deficiency, improved mechanical properties Wróbel-Kwiatkowska et al (2007b) increased flavonoid content connected with Fusarium resistance Lorenc- Kukula et al (2007) 2008 introduction of crtB gene into flax, enrichment of carotenoids in flax seed Fujisawa et al (2008) reduction of pectin content, higher retting efficiency of transgenic fibres. Musialak et al (2008) 2009 glutathion synthesis, tolerance to oxidative stress and Fusarium tolerance Czuj et al (2009) Vrbová et al (2013) 2013 the expression of chimeric gfp-TUA6 used for visualisation of microtubules Shysha et al (2013) 2014 RNAi silencing of pinoresinol lariciresinol reductase gene (LuPLR1), failed accumulation of SDG Renouard et al (2014) 2015 high oleic flax through RNAi-mediated multiple FAD2 gene silencing Chen et al (2015) agent in flax transformation is the recovery of a great number of escapes. Non-transformed shoots often originate from unmodified cells protected against selection agents by surrounding transformed cells (Jordan & McHughen 1988;McHughen 1989).…”

Section: Selective and Reporter Genes Used For Flax Transformationmentioning

confidence: 99%

“…Arabidopsis thaliana (Stoutjesdijk et al 2002), tobacco (Yang et al 2006), cotton plant , soybean (Chen et al 2011;Wagner et al 2011), rape (Jung et al 2011;Tian et al 2011) and rice (Zaplin et al 2013). Very recently, this approach was also reported in flax (Chen et al 2015). Flax transformations focused on RNA interference of fad2 gene were performed also in Agritec and the experiments with the aim to change the fatty acid content and composition are still carried out (Ludvíková et al 2014).…”

Section: Modification Of Fatty Acid Content and Composition In Flaxmentioning

confidence: 99%

Transgenic flax/linseed (Linum usitatissimum L.) - expectations and reality

Ludvíková¹,

Griga²

2015

Czech J. Genet. Plant Breed.

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Ludvíková M., Griga M. (2015): Transgenic flax/linseed (Linum usitatissimum L.) -expectations and reality. Czech J. Genet. Plant Breed., This review summarizes the history, important milestones, current status and perspectives of biotech flax/ linseed (Linum usitatissimum L.), supplemented with some of our original research, breeding and data on environmentalsafety. We show how recent biotechnology methods and genetic engineering contributed to the flax/linseed breeding in order to speed up the breeding process (doubled haploids technology; in vitro selection with the use of pathogenic toxins or heavy metals; genetic transformation) and for the creation of new flax/ linseed cultivars. The focus is laid on genetic engineering which represents an excellent technology to enrich the flax/linseed genepool with genes of interest, which are not naturally present in the flax/linseed genome. Different methods of flax transformation are mentioned, as well as various genes of interest that have been used for flax transformation to date aimed at improving transgenic flax properties, affecting both qualitative and quantitative traits. The fatty acid content and composition, the lignan (especially secoisolariciresinol diglucoside -SDG) content, flax fibre quality, tolerance to herbicides and resistance to diseases belong, among others, to flax traits that have already been modified by genetic engineering. Selection genes, reporter genes and also promoters that have been used for the vector construction are also summarized. This paper describes different fields of utilization of genetically modified (GM) flax with different improved properties. The history of the only so far officially registered transgenic linseed cultivar Triffid is described in detail. Finally, potential risks and benefits of flax modification are evaluated and also the prime expectations of GM flax and real current state of this technology compared. Unfortunately, the products created by this technology are under strict (albeit not scientifically-based) legislative/political control in the European Union (EU), which prevents the access of products, created by breeders using this top technology, to the EU market.

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“…Chen et al (2015) developed high oleic acid flax by simultaneous RNAi silencing of two FAD2 genes. RNAimediated gene silencing of the Gossypium hirsutum FAD2-1 gene raised the oleic acid content from 15 to 77 % in cotton (Liu et al 2002b).…”

Section: Introductionmentioning

confidence: 99%

Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing

et al. 2016

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Simultaneous RNAi silencing of the FAD2 and FAE1 genes in the wild species Lepidium campestre improved the oil quality with 80 % oleic acid content compared to 11 % in wildtype. Field cress (Lepidium campestre) is a wild biennial species within the Brassicaceae family with desirable agronomic traits, thus being a good candidate for domestication into a new oilseed and catch crop. However, it has agronomic traits that need to be improved before it can become an economically viable species. One of such traits is the seed oil composition, which is not desirable either for food use or for industrial applications. In this study, we have, through metabolic engineering, altered the seed oil composition in field cress into a premium oil for food processing, industrial, or chemical industrial applications. Through seed-specific RNAi silencing of the field cress fatty acid desaturase 2 (FAD2) and fatty acid elongase 1 (FAE1) genes, we have obtained transgenic lines with an oleic acid content increased from 11 % in the wildtype to over 80 %. Moreover, the oxidatively unstable linolenic acid was decreased from 40.4 to 2.6 %, and the unhealthy erucic acid was reduced from 20.3 to 0.1 %. The high oleic acid trait has been kept stable for three generations. This shows the possibility to use field cress as a platform for genetic engineering of oil compositions tailor-made for its end uses.

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Development of high oleic oil crop platform in flax through RNAi-mediated multiple FAD2 gene silencing

Cited by 55 publications

References 30 publications

Biochemical diversity of fatty acid composition in flax from VIR genetic collection and effect of environment on its development

Biochemical diversity of fatty acid composition in flax from VIR genetic collection and effect of environment on its development

Transgenic flax/linseed (Linum usitatissimum L.) - expectations and reality

Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing

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