1993
DOI: 10.1016/0093-691x(93)90362-9
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Dimethyl-acetamide as a cryoprotectant for rainbow trout spermatozoa

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Cited by 42 publications
(20 citation statements)
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“…Spermatozoa density may also effect the fertilization rate (Aas et al, 1991). Findings on spermatozoa density varied between (9.7±0.3x10 9 ml -1 and 11.3±0.5x10 9 ml -1 ) and corroborated with the results of Munkittrick and Moccia (1987) and McNiven et al (1993) but not with the findings of Buyukhatipoglu and Holtz (1984). The differences may be due to dilution ratio, age or spawning season.…”
Section: Discussionsupporting
confidence: 70%
See 1 more Smart Citation
“…Spermatozoa density may also effect the fertilization rate (Aas et al, 1991). Findings on spermatozoa density varied between (9.7±0.3x10 9 ml -1 and 11.3±0.5x10 9 ml -1 ) and corroborated with the results of Munkittrick and Moccia (1987) and McNiven et al (1993) but not with the findings of Buyukhatipoglu and Holtz (1984). The differences may be due to dilution ratio, age or spawning season.…”
Section: Discussionsupporting
confidence: 70%
“…The mean semen volumes of all experimental groups in this study was lower from most of other results reported by McNiven et al (1993), Lahnsteiner et al (1993) and also Geffen and Evans (2000) for rainbow trout. On the other hand, according to results of the present study it seems that photoperiod regimes have affected semen volume positively when compared with the control group.…”
Section: Discussioncontrasting
confidence: 55%
“…Cryopreservation of rainbow trout sperm is usually achieved with DMSO (Stoss and Holtz, 1983; Stein et al., 1989; Ciereszko and Dabrowski, 1996; Tekin et al., 2003), methanol (Lahnsteiner et al., 2002), DMA (Gallant and McNiven, 1991; McNiven et al., 1993; Babiak et al., 2001), DMSO–glycerol mixtures (Lahnsteiner et al., 1995, 1997) and ethylene glycol (Babiak et al., 2001) as permeating cryprotectants. Our results confirm DMSO and DMSO–glycerol mixtures as useful for cryopreservation of pelleted rainbow trout semen resulting in eyed eggs (49.3%) obtained with spermatozoa frozen with glucose extender containing 15% DMSO + 1% glycerol.…”
Section: Discussionmentioning
confidence: 99%
“…Generally, two types of extenders are in use for the cryopreservation of fish spermatozoa: seminal plasma‐mimicking media, and simple carbohydrate‐based solutions (Erdahl and Graham, 1980; Holtz, 1993; Ciereszko and Dabrowski, 1996). Usually dimethyl sulphoxide (DMSO) is applied as the internal cryoprotective agent, but other cryoprotectants such as dimethyl acetamide (DMA), ethylene glycol, glycerol and DMSO–glycerol mixtures are also considered as providing efficient results (Gallant and McNiven, 1991; McNiven et al., 1993; Lahnsteiner et al., 1995; Babiak et al., 2001). Especially the use of an appropriate cryoprotectant solution prevents cells from cellular disruption and membrane damage during freezing and thawing.…”
Section: Introductionmentioning
confidence: 99%
“…These results indicate that 10 MeOH and 90 FBS is the appropriate diluent for cryopreservation of ayu spermatozoa, that samples should be cooled at 42.5°C/min to -50°C and that the spermatozoa should be used for artiˆcial fertilization immediately after thawing. 9 11) glycerol, 12,13) MeOH, 14 16) N,N dimethylacetamide (DMA), 17,18) …”
mentioning
confidence: 99%