Do Sertoli cells secrete fluid into the seminiferous tubules?

Bp, Setchell

doi:10.1530/jrf.0.0190391

Cited by 26 publications

(4 citation statements)

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“…The TF is generated principally by Sertoli cells [37] . Sertoli cells clearly secrete active compounds into this fluid, but the release of soluble factors into the TF by germ cells has never been directly studied and documented.…”

Section: Resultsmentioning

confidence: 99%

An Integrative Omics Strategy to Assess the Germ Cell Secretome and to Decipher Sertoli-Germ Cell Crosstalk in the Mammalian Testis

et al. 2014

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Mammalian spermatogenesis, which takes place in complex testicular structures called seminiferous tubules, is a highly specialized process controlled by the integration of juxtacrine, paracrine and endocrine information. Within the seminiferous tubules, the germ cells and Sertoli cells are surrounded by testicular fluid (TF), which probably contains most of the secreted proteins involved in crosstalk between these cells. It has already been established that germ cells can modulate somatic Sertoli cell function through the secretion of diffusible factors. We studied the germ cell secretome, which was previously considered inaccessible, by analyzing the TF collected by microsurgery in an “integrative omics” strategy combining proteomics, transcriptomics, genomics and interactomics data. This approach identified a set of proteins preferentially secreted by Sertoli cells or germ cells. An interaction network analysis revealed complex, interlaced cell-cell dialog between the secretome and membranome of seminiferous cells, mediated via the TF. We then focused on germ cell-secreted candidate proteins, and we identified several potential interacting partners located on the surface of Sertoli cells. Two interactions, APOH/CDC42 and APP/NGFR, were validated in situ, in a proximity ligation assay (PLA). Our results provide new insight into the crosstalk between germ cells and Sertoli cells occurring during spermatogenesis. Our findings also demonstrate that this “integrative omics” strategy is powerful enough for data mining and highlighting meaningful cell-cell communication events between different types of cells in a complex tissue, via a biological fluid. This integrative strategy could be applied more widely, to gain access to secretomes that have proved difficult to study whilst avoiding the limitations of in vitro culture.

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Section: Resultsmentioning

confidence: 99%

An Integrative Omics Strategy to Assess the Germ Cell Secretome and to Decipher Sertoli-Germ Cell Crosstalk in the Mammalian Testis

et al. 2014

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“…In kidney, gelsolin is predominantly concentrated in the apical web of principal cells of the collecting duct (Hartwig et al, 1990); such a localization has been interpreted as a role for gelsolin in the vasopressin-induced changes of the actin cytoskeleton leading to an increased water permeability of the apical plasma membrane. By analogy with cells of the kidney, it may be suggested that rearrangements of apical actin filaments under the action of gelsolin in Sertoli cells may be important for tubule fluid formation, an important function of these cells (Setchell, 1969;Fawcett, 1975, Waites andGladwell, 1982;Jégou, 1992Jégou, , 1993Jégou et al, 1982Jégou et al, , 1983. Another possible role for apical gelsolin could be related to the phagocytosis of residual bodies by Sertoli cells, a process presumably involved in the recycling of cellular materials and in the regulation of some aspects of Sertoli cell function (Jégou, 1991(Jégou, , 1993Jégou et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Distribution of gelsolin in human testis

Rousseaux-Prévost¹,

Delobel²,

Hermand

et al. 1997

Mol. Reprod. Dev.

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Gelsolin, an actin‐binding and severing protein present in many mammalian cells, was characterized in human testis. Although abundant in testicular extracts, gelsolin was not detected in purified spermatogenic cells by immunoblot analysis. Immunofluorescence studies of testis sections showed that gelsolin has two main localizations: peritubular cells and the seminiferous epithelium. In peritubular cells, gelsolin was present together with α‐SM actin, in agreement with the myoid cell characteristics of these cells. In a large proportion of the tubules, gelsolin was found mainly, together with actin, in the apical part of the seminiferous epithelium. This localization of gelsolin also was observed in seminiferous tubules with a partial or complete absence of germinal cells, which evokes a presence of gelsolin at the apex of Sertoli cells. However, in normal testis, a complex pattern of gelsolin labeling was also present, mostly in the apical third of the epithelium, around cells or groups of cells, mainly spermatids, and, less frequently, in various other localizations from the apical to the basal part of the seminiferous epithelium. Taken together, these observations suggest that gelsolin may play different functions in the seminiferous epithelium: (1) regulation of the dynamic alterations of the actin cytoskeleton in the apical cytoplasm of Sertoli cells, and (2) modification of actin filaments assemblies in specific structures at germ cell‐Sertoli cell contacts. Thereby, the actin‐modulating properties of gelsolin are probably involved in reorganization of the seminiferous epithelium related to germ cell differentiation. Mol. Reprod. Dev. 48:63–70, 1997. © 1997 Wiley‐Liss, Inc.

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“…They provide nutrients and essential molecules to the differentiating germ cells and regulate the seminiferous tubular fluid (Griswold, 1998;Rato et al, 2010). The Sertoli cells are interconnected by different junctions, creating a unique barrier between surrounding blood vessels and differentiating germ cells that is known as the "blood-testis barrier" (Cheng and Mruk, 2002;Dym and Fawcett, 1970;Setchell, 1969;Vogl et al, 2008). This barrier restricts molecules to enter or exit the adluminal compartment, creating a microenvironment with diverse transporters and preventing immunological response against germ cells (reviewed in Mital et al, 2011).…”

Section: Spermatogenesismentioning

confidence: 99%