Effect of quality and quantity of dietary protein on free amino acids in plasma and tissues of adult rats.

Fujita, Yoshiaki; Yamamoto, Takashi; Rikimaru, Toru; Ebisawa, Hidemichi; Inoue, Goro

doi:10.3177/jnsv.27.129

Cited by 14 publications

(3 citation statements)

References 17 publications

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“…Amino acid concentrations in serum and plasma can give indications of an animals' physiological protein status. An increase in NEAA (non-essential amino acids) is indicative of an animal in a state of protein deficiency leading to muscle catabolism (Fujita et al, 1981). In the current study when cattle were fed 90% MP requirements, they had increased NEAA concentrations of Gly and tended to have higher Ser concentrations compared to cattle fed 100% or 110% their MP requirements.…”

Section: Serum Amino Acid Concentrationsmentioning

confidence: 51%

76 Does supplemental protein and rumen-protected methionine improve performance and digestibility during late-gestation in beef cows?

Collins

Shoveller

Mandell

et al. 2019

Journal of Animal Science

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Methionine (MET) is likely the first limiting amino acid in low-protein forages fed to beef cattle during late-gestation. Therefore, the objective of this study was to determine if supplemental protein and rumen-protected MET improve cow performance and apparent tract digestibility (TTD) during late-gestation. This study used 147 late-gestation Angus crossbred cows and heifers in a 3x2 factorial arrangement for dietary treatments. The cattle were randomly assigned to one of six diets formulated to 90, 100 or 110% of metabolizable protein (MP) requirements (NRC, 2016), with (without) 9 g/d of rumen-protected MET (MetaSmart, Adisseo Inc.). These diets were fed for approximately 8 wks before calving. All data was analyzed as a randomized block design using SAS 9.4 PROC GLIMMIX procedure. Cows fed at 90% MP requirements lost body weight (BW), while cows fed at 100% and 110% MP requirements maintained and (or) gained BW over the trial (P = 0.02). Similarly, cows fed at 90% MP requirements lost more pregnancy corrected BW than cows fed at 100% and 110% MP requirements (P = 0.01). However, supplemental MET did not affect body weight gains (P > 0.07). Cows and heifers fed at 90% MP requirements had reduced TTD for crude protein compared to cattle fed to 110% MP requirements (P < 0.001), MET supplementation did not impact TTD (P > 0.20). Additionally, cattle fed at 90% MP requirements had increased serum cholesterol and reduced urea concentrations compared to cows fed to 100% and 110% MP requirements (P < 0.0001). MET supplementation increased serum concentrations for glucose, isoleucine, leucine, lysine, serine, threonine and valine (P < 0.02). Calf birth weights were not significantly impacted by dietary treatment (P > 0.31). Feeding cows above their MP requirements may improve late-gestation performance and CP digestibility. Supplemental MET may increase amino acid utilization but did not improve beef cow performance or digestibility parameters measured in late-gestation.

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Section: Serum Amino Acid Concentrationsmentioning

confidence: 51%

76 Does supplemental protein and rumen-protected methionine improve performance and digestibility during late-gestation in beef cows?

Collins

Shoveller

Mandell

et al. 2019

Journal of Animal Science

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“…The higher NEAA with decreased protein intake contributed to the significantly lower EAA-to-NEAA ratio observed in plasma and RBC in the 6% protein group. An increase in NEAA and a decrease in EAA are generally found during protein malnutrition and have been assumed to reflect the enhanced protein breakdown (12), which in vivo is reduced by both insulin and IGF-I ( 29).…”

Section: Discussionmentioning

confidence: 99%

Effect of protein intake on plasma and erythrocyte free amino acids and serum IGF-I and IGFBP-1 levels in rats

Filho

Hazel

Anderstam

et al. 1999

American Journal of Physiology-Endocrinology and Metabolism

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Amino acid (AA) levels in plasma and erythrocytes (RBC) were determined in rats ( n = 29) fed diets with 6, 21, and 35% protein, and their association with insulin-like growth factor I (IGF-I), insulin, or IGF-binding protein (IGFBP)-1 levels was studied. Free AA in plasma and RBC were determined by reversed-phase high-pressure liquid chromotography, and IGF-I, IGFBP-1, and insulin plasma levels were determined by RIA. Rats fed the low-protein (6%) diet were growth-retarded and had lower serum IGF-I levels and higher serum IGFBP-1 levels than the other two groups ( P < 0.0001). In rats fed the low-protein diet, most of the nonessential AA (NEAA) in both plasma and RBC increased, whereas the essential AA (EAA), with the exception of threonine, decreased. When the groups were combined, both RBC and plasma EAA-to-NEAA ratios were positively correlated to IGF-I ( r = 0.76 and 0.80, respectively; P < 0.0001) and inversely correlated to IGFBP-1 levels ( r = −0.67, P < 0.001 and r = −0.78, P < 0.0001, respectively). A significant inverse correlation was found between RBC glutamate and IGF-I ( r = −0.85, P < 0.0001, n = 25) and insulin ( r = −0.72, P < 0.001, n = 21), and a positive correlation was found for IGFBP-1 ( r = 0.78, P < 0.0001, n = 24). In multiple regression analysis, only IGF-I remained as an independent variable. Threonine was the only EAA with a significant inverse correlation to insulin ( r = −0.66, P < 0.001). We hypothesize that AA metabolism is associated to changes in IGF-I, insulin, and IGFBP-1 levels in rats on different protein intakes.

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“…The ratio of EAA to NEAA, in either the diets or the plasma, has been shown to be important in relation to protein quality and utilization in mammals (Young and Zamora 1968;Hartog and Pol 1972;Fujita et al 1981;Mercer et al 1989). During the absorptive period in Experiment 1, fish fed Diet 1 had a plasma ratio of 1, whereas plasma ratios of EAA to NEAA in fish fed Diets 2 and 3 were considerably lower, reflecting the amino acid composition of the diets.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of plasma free amino acids in rainbow trout (Oncorhynchus mykiss under variety of dietary conditions

Tantikitti

March

1995

Fish Physiol Biochem

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Plasma amino acid profiles in the systemic circulation of rainbow trout acclimated to diets containing different protein sources, with and without supplementation with free amino acids, were monitored for up to 120 hours after consumption of the diets. Plasma concentrations of lysine, methionine, and isoleucine increased more rapidly after feeding a diet supplemented with these amino acids in free form and, depending upon the increase in plasma concentration, remained at concentrations above those in fish fed the basal diet for at least 24h after feeding. Dietary supplementation with isoleucine increased plasma concentrations of leucine and valine as well as of isoleucine. Maximum plasma concentrations for most amino acids were attained between 12 and 24h postprandial. Dietary inclusion of gelatin caused more rapid elevations in plasma glycine and serine than did free glycine. Feeding at three hour intervals resulted in stable plasma concentrations of individual amino acids in contrast to the fluctuations occurring when fish were fed once daily. Frequent feeding favoured a higher proportion of protein to lipid in body composition.

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Effect of quality and quantity of dietary protein on free amino acids in plasma and tissues of adult rats.

Cited by 14 publications

References 17 publications

76 Does supplemental protein and rumen-protected methionine improve performance and digestibility during late-gestation in beef cows?

76 Does supplemental protein and rumen-protected methionine improve performance and digestibility during late-gestation in beef cows?

Effect of protein intake on plasma and erythrocyte free amino acids and serum IGF-I and IGFBP-1 levels in rats

Dynamics of plasma free amino acids in rainbow trout (Oncorhynchus mykiss under variety of dietary conditions

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