Effect of Statins with α-Tricalcium Phosphate on Proliferation, Differentiation, and Mineralization of Human Dental Pulp Cells

Varalakshmi, Parasuraman R.; Kavitha, Mahendran; Govindan, Ramajayam

doi:10.1016/j.joen.2012.12.036

Cited by 42 publications

(51 citation statements)

References 34 publications

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“…On the contrary, when the concentrations of WPLTs and PRP reached 500 mL/L, their role in promoting cell proliferation was significantly reduced compared with that of the low concentration group. Previously, many researchers have also observed that the role of blood platelets in promoting cell proliferation reduced when the concentration of blood platelets was increased beyond a certain level (Varalakshmi et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

2015

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ABSTRACT. We examined the effects of washed platelets (WPLTs) and platelet-rich plasma (PRP) on the proliferation and mineralization of rat dental pulp cells. Rat dental pulp cells were separated, cultured, and identified. Medium containing 1, 10, 100, or 500 mL/L PRP or WPLTs was added to 4th generation cells. The MTS method was used to determine cell proliferation. Alizarin red staining was used to observe the formation of mineralized nodules after cell mineralization and induction for 10 and 20 days under different culture conditions, and the areas of the mineralized nodules formed 20 days after induction were computed. The addition of 1, 10, and 100 mL/L WPLTs or PRP significantly promoted rat dental pulp cell proliferation (P < 0.05) whereas 500 mL/L WPLTs or PRP had no significant effect (P > 0.05). Under the same concentrations, no significant differences on cell proliferation were observed between WPLT and PRP treatments (P > 0.05 in all groups). After 10 days mineralization and culture, the 100 and 500 mL/L WPLT and PRP group positive nodule rates were significantly higher than those of the low concentration and the control groups (P < 0.05). After 20 days, the areas of the mineralized nodules formed in the 100 and 500 mL/L WPLT and 9487 Effects of WPLTs and PRP on rat dental pulp cells ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 9486-9496 (2015) PRP groups were significantly larger than those in the control group (P < 0.05). These results demonstrate that both WPLTs and PRP are equally able to significantly promote the proliferation and calcification of rat dental pulp cells under a certain range of concentrations.

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Section: Discussionmentioning

confidence: 99%

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

2015

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“…41 Varalakshimi et al reported that when simvastatin is used in association with tricalcium phosphate (TCP) carrier, there is an increase in the expression of Osteocalcin in hDPSCs. 42 Evaluation of changes in the expression of DSPP gene, too, showed that incorporation of simvastatin alone and in association with hydroxyapatite resulted in a significant increase in the expression of DSPP gene. Karanxha et al concluded in a similar study that incorporation of simvastatin in association with enamel matrix derivatives resulted in an increase in osteoblastic differentiation by increasing the expression of DSPP gene.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold

Samiei¹,

Aghazadeh²,

Aminabadi³

et al. 2016

Adv Pharm Bull

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“…1,2 This class of drug can inhibit HMG-CoA reductase from turning into mevalonic acid, and is widely used for hyperlipidemia treatment. 1,2 Several researchers have demonstrated that SV has pleiotropic effects, including anti-inflammatory action, 2,3 induces angiogenesis and improves the function of endothelial cells, 4,5,6,7,8 as well as enhancing the synthesis and mineralization of bone 9,10,11,12,13 and dentin 6,14,15,16,17 matrices. The pathway for SV mediating osteo/odontogenic differentiation is not fully known.…”

Section: Introductionmentioning

confidence: 99%

Biostimulatory effects of simvastatin on MDPC-23 odontoblast-like cells

et al. 2017

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The aim of this study was to evaluate the bioactivity and cytocompatibility of simvastatin (SV) applied to MDPC-23 odontoblast-like cells. For this purpose, MDPC-23 cells were seeded in 96-well plates and submitted to treatments with 0.01 or 0.1 µM of SV for 24 h, 72 h or continuously throughout the experimental protocol. The negative control group (NC) was maintained in DMEM. Cell viability (MTT), ALP activity (thymolphthalein monophosphate), and mineralized matrix deposition (alizarin red) were analyzed at several time points. The data were submitted to ANOVA and Tukey's test (α = 0.05). Although cell viability was observed in the groups treated with SV, these groups did not differ from the NC up to 7 days. There was a reduction in cell viability for the groups treated with 0.1 µM of SV for 72 h, and submitted to continuous mode after 14 days. A significant increase in ALP activity occurred in the group treated with 0.01 µM of SV for 24 h, compared with the NC; however, only the group treated with 0.1 µM of SV in continuous mode reduced the ALP activity, in comparison with the NC. After 14 days, only continuous treatment with 0.1 µM of SV did not differ from NC, whereas the other experimental groups showed increased mineralized matrix deposition. Thus, it was concluded that low concentrations of simvastatin were bioactive and cytocompatible when applied for short periods to cultured MDPC-23 odontoblast-like cells.

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Effect of Statins with α-Tricalcium Phosphate on Proliferation, Differentiation, and Mineralization of Human Dental Pulp Cells

Cited by 42 publications

References 34 publications

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold

Biostimulatory effects of simvastatin on MDPC-23 odontoblast-like cells

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