Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843)

Abstract

This study examined the e¡ects of storage time and cryoprotectant concentrations on the post-thaw sperm of red seabream, Pagrus major. Sperm treated with 12%, 15%, 18% and 21% DMSO were cryopreserved for10,30,60 and 360 days, and fertilization and hatching rates were analysed. For all groups, there were no di¡erences in the fertilization rates and hatching rates between sperm cryopreserved for o60 days and fresh sperm (98.8 AE 0.8%, 96.4 AE 1.3%). However, for sperm cryopreserved for 360 days, both fertilization rates (88.6 AE 3.0% to 7.0 AE 1.9%) and hatching rates (79.4 AE 7.2% to 3.3 AE 0.8%) decreased drastically. Furthermore, the cryoprotectant concentrations affected sperm quality signi¢cantly (Po0.05). When cryopreserved for 360 days, sperm treated with 15% DMSO obtained the best results compared with other concentrations.We suggest that 15% DMSO may be an e¡ective cryoprotectant for long-term sperm cryopreservation of red seabream. Aquaculture Research, 2010, 41, e89^e95 Changes of sperm quality after cryopreservation Q H Liu et al. r 2010 The Authors Journal Compilation r 2010 Blackwell Publishing Ltd, Aquaculture Research, 41, e89^e95 e91Changes of sperm quality after cryopreservation Q H Liu et al.

Sperm motility evaluation following long-term storage (5 years) of cryopreserved sea bream (Sparus aurata L., 1758) semen

“…On the other hand, also in the case of human sperm, where cryopreservation is actually a routine technique and cryopreserved semen has been successfully used after very long storage times (Feldschuh et al., ), cryo injuries related to the liquid nitrogen storage time have been observed (Desrosiers et al., ). Also for fish, different effects of long‐term storage were reported; in fact, while no effects on post‐thaw motility and fertilization rate were observed for storage periods of up to 1 year in Pagrus major (Liu et al., ) and Psetta maxima (Suquet et al., ), a decrease in antioxidant activity and a corresponding decline in longevity, motility and fertilization ability have been observed in sperm of the red sea bream P. major by Chen et al. () after 48 months of storage.…”

“…However, as these evaluations usually seek to identify cryodamage affecting sperm cells as a consequence of the freezing‐thawing process, they are usually performed after short‐term preservation in liquid nitrogen, or even without taking the length of storage time into account. Only a few studies have specifically evaluated the effects of shorter or longer storage times in liquid nitrogen on the viability of thawed sperm (Suquet et al., ; Chen et al., ; Liu et al., ).…”

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

“…(Suquet et al 2000). For this reason, effective sperm cryopreservation procedure has been adviced to the aquaculture sector to improve their culture techniques (Liu et al 2010).…”