Effects of eutrophication on vitellogenin gene expression in male fathead minnows (Pimephales promelas) exposed to 17α-ethynylestradiol in field mesocosms

Characterization of vtg-1 mRNA expression during ontogeny in Oreochromis mossambicus (PETERS)

“…The first instance represented a widely used system in which the low background expression of vtg in adult male liver forms the basis of the screening assay. Examples of this can be found for adult tilapia (O. aureus and O. mossambicus) and other teleost males [27,33,61,[67][68][69][70][71]. In accordance with the present work on O. mossambicus, these studies demonstrated relatively low levels of vtg expressed in the liver prior to estrogen-associated induction.…”

A new approach for the laboratory culture of the fathead minnow, Pimephales promelas

“…Recent interest in the molecular‐level effects of pharmaceuticals and personal care products in the aquatic environment, as well as targeting molecular changes in specific organs in sentinel species, has resulted in the need for large numbers of adult fathead minnow males and females for use in these studies . Current gene expression and proteomics studies with adult fathead minnows conducted in our laboratory have required the use of as many as 500 adult fathead minnow males for a single experiment, and a similar increase has been observed in tests that require larval fish . For example, within our lab, as many as 3000 fathead minnow larvae of the same age are required for the bioassessment of mine waste serial dilution samples .…”

“…The fathead minnow ( Pimephales promelas ) is used routinely as a test organism for aquatic toxicity testing of pure chemicals, effluents, and receiving waters . Various fathead minnow testing methodologies describe the use of animals ranging from embryos to sexually mature adults, as well as every possible life stage in between these extremes .…”

Avaliação da atividade estrogênica das águas do rio Itapecuru no município de Bacabeira-MA

“…Em peixes, a desregulação endócrina causada por xenoestrogênios foi correlacionada a feminização (RODGER-GRAY et al, 2001), alteração nas gônadas (PANTER et al, 1999), hermafroditismo (KOGER et al, 2000), incidência de testículos-óvulos nas gônadas (KANG et al, 2002) e indução à síntese de vitelogenina (PANTER et al, 1999, ROSE et al, 2002GORDON et al, 2006;HINCK et al, 2006;REMPEL et al, 2006 Para análise eficiente de como determinada substância irá agir fisiologicamente em um organismo, o mais indicado é utilizar os métodos analíticos juntamente com os biomarcadores (BUSS et al, 2003;ARIAS et al, 2007).…”

“…A proteína vitelogenina (VTG) demonstra bons resultados e é frequentemente usada como biomarcador quando o intuito da pesquisa é verificar efeitos estrogênicos de desreguladores endócrinos (ARUKWE; GOKSOYR, 2003;GORDON et al, 2006;HINCK et al, 2006;REMPEL et al, 2006). Ela é produzida no fígado de animais, por meio do estímulo causado pela presença do estrógeno 17β-estradiol, para compor o vitelo presente nos ovócitos (ARUKWE; GOKSOYR, 2003;LANGSTON et al, 2005).…”