Effects of fluid shear stress on expression of focal adhesion kinase in MG-63 human osteoblast-like cells on different surface modification of titanium

Abstract

This study aimed to investigate the effect of fluid shear stress (FSS) on cell proliferation and expression of focal adhesion kinase (FAK) in MG-63 cells on different modified titanium surfaces. MG63 cells were cultured on three different surfaces: glass slide, polished treatment (PT) titanium surface and sandblasted/acid-etched surfaces (SLA) titanium surface. The surface topography and roughness were evaluated by scanning electron microscopy (SEM) and atomic force microscopy (AFM), respectively. The cells were subjected to FSS, and the cell appearance before and after the stress was evaluated. MTT assay was applied to estimate cell proliferation. The mRNA and protein levels of FAK were determined by qRT-PCR and western blotting. Titanium plates demonstrated different surface microtopography. Parameter Ra values of SLA group were around 3.4 µm, which was higher than PT group. Exposure to the FSS of 12 dynes/cm 2 significantly induced positive upregulation of cellular proliferation and the expression of FAK, which were directly correlated with the duration of exposure and surface. Cells in SLA group were able to endurance the longtime of FSS, especially under the FSS of 16 dynes/cm 2 . SLA surface had a positive influence on the expression of FAK. Different surface modifications created different microtopography of titanium plates. Cell proliferation and the mRNA and protein expression of FAK were stimulated by FSS and regulated by a marked synergistic effect of surface topography and the level and duration of FSS.

Effect of Splinting on Orthodontic Mini-Implant Tipping and Bone Histomorphometric Parameters: An In Vivo Animal Model Study

“…In this regard, active bone cells-including both osteoblasts and osteoclasts, as well as their respective precursors-have been shown to activate distinct mechanical transduction pathways that are known to modulate cellular behavior, including proliferation, differentiation, and functional activity, in response to distinct forms of mechanical stress of the local microenvironment, in which both compressive and tensile stresses are included [65]. Of additional relevance, the induction of fluid shear stress-by the flow of tissue fluid at the lacunar-canalicular bone system [66]-has recently been found to modulate the mechanical transduction of the osteoblastic behavior at the bone-to-implant interface, via the enhancement of the cell proliferation and differentiation [67], potentially contributing to the attained outcomes.…”

Effects of Mechanical Stress Stimulation on Function and Expression Mechanism of Osteoblasts

“…Mechanical stimulation is an important regulatory factor for bone growth, reconstruction, and metabolism. Number experimental studies have investigated osteogenic effects under mechanical stress ( Chermside-Scabbo et al, 2020 ; Eichholz et al, 2020 ; Jeon et al, 2021 ; Lei et al, 2021 ). The influence of different mechanical forces on bone tissue produces various effects.…”

“…Recently, the in-depth study of bone ingrowth between internal fixation materials and bone interface has encouraged the study of material-bone interface-mechanical stimulation as an emerging focus. Lei et al(2021) reported the effect of FSS on human MG-63 osteoblast-like cells on titanium with different surface modifications, which was particularly evident on the implant-bone interface. This study found that FSS (12 dyn/cm 2 ) significantly induced cell proliferation and upregulated the expression level of focal adhesion kinase (FAK), which the authors speculated that FAK may play a key role in the mechanical transduction of the implant-bone interface ( Lei et al, 2021 ).…”

Preparation and characterization of scutellarin loaded on ultradeformable nano-liposomes scutellarin EDTMP (S-UNL-E) and in vitro study of its osteogenesis

“…Staining working liquid was added and incubated at room temperature in the dark for 30 min. Cells with alkaline phosphatase activity were dyed purple-black or blue-black [ 22 ].…”