Effects of Nickel on Human and Fish Red Blood Cells

Luca, G. De; Gugliotta, T.; Parisi, Giulia; Romano, P.; Geraci, Antonella; Romano, Orazio; Scuteri, A; Romano, L.

doi:10.1007/s10540-007-9053-0

Cited by 26 publications

(18 citation statements)

References 24 publications

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“…Sulphate influx measured in Liza aurata and Salmo irideus erythrocytes. 34 These data support the use of kinetics of flux measured in the fish RBCs to assess the activity of anion exchange operating from band 3, which appears in marked manner in RBCs of L. aurata and S. irideus. Bars represent mean AE SD in percentage for temperature, pH and dissolved oxygen (which ranged between 10.97% and 33.33%) and significant changes in percentage for salinity (100%).…”

Section: Discussionsupporting

confidence: 73%

Oxidative stress and band 3 protein function in Liza aurata and Salmo irideus erythrocytes: effect of different aquatic conditions

Casella

Ielati

Piccione

et al. 2012

Cell Biochemistry & Function

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The aim of this study was to assess the effect of the different aquatic conditions on anion transport of fish erythrocytes through the measurement of the sulphate uptake operating from band 3, the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG). To this purpose, blood samples of 30 Liza aurata and 30 Salmo irideus fishes were collected via caudal vein and washed with physiological buffer. Successively, erythrocytes suspended at 3% haematocrit were used to measure the SO(4) (=) influx by atomic absorption spectrophotometry at 425 nm wavelength and the GSH concentration using an immunodiagnostic assay intended for the quantitative determination of glutathione in ethylenediaminetetraacetic acid in blood. All results were analyzed using unpaired Student's t-test and a P < 0.05 was considered statistically significant. The results of this study showed statistically significant differences about the sulphate uptake and the glutathione levels in S. irideus with respect to L. aurata (P < 0.0001). In conclusion, the different aquatic conditions play an important role on anion transport in fish erythrocytes, showing that environmental differences induce perturbations in erythrocyte membrane and should be evaluated to prevent physiological damages in fishes.

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Section: Discussionsupporting

confidence: 73%

Oxidative stress and band 3 protein function in Liza aurata and Salmo irideus erythrocytes: effect of different aquatic conditions

Casella

Ielati

Piccione

et al. 2012

Cell Biochemistry & Function

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“…In the late exposure period, the exposed GSH/ GSSG ratio was significantly different from the control, which might highlight that Ni exerts toxicity on the treated copepods via alteration of the cellular redox state. Similarly, some authors attribute the toxic effects of Ni to a decrease in cellular GSH and a concomitant increase in GSSG, altering the redox state of the cells (De Luca et al 2007). Thus, GSH/GSSG could be a suitable biomarker for oxidative stress or injury in biological organisms (Hwang et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Wang

2009

Ecotoxicology

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Tigriopus japonicus Mori has been recognized as a good model for toxicological testing of marine pollutants. Recently, a large number of genes have been identified from this copepod, and their mRNA expression has been studied independently against exposure to marine pollutants; however, biochemical-response information is relatively scarce. The response of T. japonicus to nickel (Ni) additions was examined under laboratory-controlled conditions in 12 days exposure. Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), acetylcholinesterase (AchE), reduced glutathione (GSH), the ratio of reduced to oxidized glutathione (GSH/GSSG) and metallothionein (MT) were analyzed for Ni treatments (0, 0.125, 0.25, 0.75 and 3.0 mg/L) after 1, 4, 7 and 12 days. The thiobarbituric reactive species assay was used to evaluate lipid peroxidation (LPO) level in copepods after exposure. The results showed that Ni remarkably affected the biochemical parameters (SOD, GPx, GST, GSH, and GSH/GSSG) after certain exposure durations. However, the copepod's LPO level was significantly decreased under metal treatments after exposure, hinting that the factors involved in LPO might not significantly depend on the operations and functions in the antioxidant system. Ni exhibited the neurotoxicity to copepods, because its use obviously elevated AchE activity. During exposure, Ni initially displayed an inhibition effect but induced MT synthesis in T. japonicus by day 12, probably being responsible for metal detoxification. Thus, Ni had intervened in the detoxification process and antioxidant system of this copepod, and it could be used as a suitable bioindicator of Ni exposure via measuring SOD, GPx, GST, and MT as biomarkers.

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“…Because of its position, the protein is continuously damaged by chemical agents and drugs circulating in the blood. Previous studies reported that some metals enhance the degradation of band 3 protein after it has been treated with chemical substances likely to affect sulphate permeability and modulate anion influx through the erythrocyte membranes [22]. Oxidation, caused by high concentrations of lead chloride, changed the -SH groups into SS-groups, resulting in a conformational change in the protein structure.…”

Section: Discussionmentioning

confidence: 99%

Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

Gugliotta

Luca

Romano

et al. 2012

Cellular and Molecular Biology Letters

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Our study concerns the effects of exposure to lead chloride on the morphology, K + efflux, SO 4 -influx and GSH levels of the human erythrocyte. Blood was collected in heparinized tubes and washed three times. The cells were suspended at 3% hematocrit and incubated for 1 h at 25ºC in a medium containing increasing concentrations of lead chloride (0, 0.3, 0.5 and 1 µM). After incubation, the suspensions were centrifuged and the erythrocyte pellets were divided into three aliquots for testing. The results show: an increase in the permeability of erythrocytes treated with lead chloride with consequent damage and cellular death, especially in the presence of high concentrations; an increase in potassium ion efflux; alterations in the morphology and membrane structure of the red blood cells; and a decrease in sulphate uptake, due either to the oxidative effect of this compound on the band 3 protein, which loses its biological valence as a carrier of sulphate ions, or to a decrease in the ATP erythrocyte concentration. In conclusion, the exposure of erythrocytes to Pb

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Effects of Nickel on Human and Fish Red Blood Cells

Cited by 26 publications

References 24 publications

Oxidative stress and band 3 protein function in Liza aurata and Salmo irideus erythrocytes: effect of different aquatic conditions

Oxidative stress and band 3 protein function in Liza aurata and Salmo irideus erythrocytes: effect of different aquatic conditions

Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

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