Elicitor-enhanced Silymarin production in hairy root cultures of Silybum marianum L. Gaertn

Hasanloo, Tahereh; Rahnama, Hasan; Sepehrifar, R.; Shams, Mohammad Reza

doi:10.1055/s-0028-1084652

Cited by 9 publications

(8 citation statements)

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“… Hasanloo et al (2013) studied the effects of different amounts of fungal elicitors on the cultivation of hairy roots of S. marianum . The study showed an enhanced production of flavonoids and improved root growth compared to the control hydroponics ( Hasanloo et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, AgNPs play an important role in plant growth as they interfere with different pathways in plant cells and regulate secondary metabolites production ( Vannini et al, 2013 ). Hasanloo et al (2013) studied the effects of different amounts of fungal elicitors on the cultivation of hairy roots of S. marianum . The study showed an enhanced production of flavonoids and improved root growth compared to the control hydroponics ( Hasanloo et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

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Hydroponics and elicitation, a combined approach to enhance the production of designer secondary medicinal metabolites in Silybum marianum

Mubeen

Hasnain

Mehboob³

et al. 2022

Front. Plant Sci.

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Medicinal plants have been used to cure human diseases since decades. Silybum marianum, a medicinal plant, is regarded as a source of secondary metabolites with therapeutic value against liver diseases and diabetes. The present study was conducted to enrich the production of secondary metabolites in the vegetative parts of Silybum marianum using elicitation strategy in hydroponic system with different elicitors. The elicitors of fungus Aspergillus niger (0.2 g/L), methyl jasmonate (MeJA) (100 μM) and silver nanoparticles (AgNPs) (1 ppm) were added in hydroponic medium, individually and in combination form to the 15 days old plant. The elicitor-treated plants were harvested at different time points (24–144 h; increment 24 h) and their biochemical parameters like phenolics, flavonoids, nitric oxide (NO), and superoxide dismutase (SOD) were analyzed. The results showed hyper-accumulation of these biochemical contents, especially in response to MeJA (100 μM), followed by AgNPs (1 ppm) and co-treatment of AgNPs (1 ppm) with other elicitors. The results revealed that the treatment with MeJA (100 μM) exhibited the highest flavonoid (304 μg g–1), phenolic (372 μg g–1), and SOD (16.2 U g–1) contents. For NO levels, the maximum value of 198.6 nmole g–1 was achieved in response to the treatment with MeJA + Green synthesized AgNPs (100 μM + 1 ppm). Our findings depicted an enhanced production of medicinally important plant secondary metabolites and antioxidants; hence, the method applied in this study can play a significant role to improve therapeutic values of the plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hydroponics and elicitation, a combined approach to enhance the production of designer secondary medicinal metabolites in Silybum marianum

Mubeen

Hasnain

Mehboob³

et al. 2022

Front. Plant Sci.

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“…Hairy roots are roots of plant tissue obtained by genetic transformation using a bacteria naturally present in the soil, Agrobacterium rhizogenes. Hairy root cultures of S. marianum have been established [122][123][124]. Elicitation experiments have been undertaken on this model.…”

Section: Regulation Of Flavonolignans Biosynthesis In Silybum Marianummentioning

confidence: 99%

Biosynthesis and Regulation of Antioxidant Flavonolignans in Milk Thistle

Drouet¹,

Hano²

2021

Antioxidants - Benefits, Sources, Mechanisms of Action

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Mature fruits (achenes) of milk thistle (Silybum marianum (L.) Gaertner, Asteraceae) accumulate high amounts of silymarin, a complex mixture of bioactive antioxidant flavonolignans deriving from taxifolin. Their biological activities in relation with human health promotion and disease prevention have been well described. The conditions of their biosynthesis in planta, however, have long been elusive and thus tend to be a limiting factor for their future applications. Significant advances in understanding their biosynthesis and regulation have been made over the last decade and are outlined in the current chapter.

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“…In fact, the three enzymes were associated with several stress-related processes including root browning, would healing, and disease resistance through the activation of plant defense responses [44]. CS elicited an increase in total flavonolignans in Silybum marianum HRs by switching on the lignification process involving the three key enzymes [45,46]. In summary, it is possible that the improvement in GIA was because PA plays a role as a source material for the production of numerous flavonoid and phenolic compounds through the reaction of phenylalanine ammonia lyase.…”

Section: Combined Effect Of Phenylalanine and Chitosan On Biomass Andmentioning

confidence: 99%

Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor

Cao

Hoang

et al. 2020

Plants

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Aeroponics is considered as a potential method for the culture of herbal plants due to the high growth rate, quantity and quality enhancement of secondary metabolites, and substantial environmental progress associated with this method. The aim of this study was to develop a sufficient protocol for successful Urena lobata hairy root induction by Agrobacterium rhizogenes ATCC 15834, using a precursor and elicitor to enhance α-glucosidase inhibitory activity (GIA) of aeroponic hairy roots (AHRs) in greenhouse conditions. In this study, we found that the optimized procedure (10 min, Woody plant medium (WPM), 1/25 salt strength) had an outstanding effect with a reduction in the rooting time (RT), promotion of the rooting rate (RR), and increase in the fresh weight (FW) and dry weight (DW) compared with the original procedure (30 min, Murashige and Skoog (MS) medium, 1/25 salt strength) after 30 days of culture. The highest DW, GIA, flavonoid (FLA) and phenolic (PHEL) contents were observed for individual addition of 10 mM phenylalanine (PA) or 50 mM chitosan (CS) in the late exponential phase (eighth week) with 15 days of elicitation compared to the control AHRs. However, individual treatment was less effective than the combination of the two. Positive correlations among the GIA, FLA and PHEL indicate that AHRs accumulated phenolic compounds, leading to an increase in the GIA by a synergistic effect. In conclusion, the culture of Urena lobata AHRs with PA and CS is an efficient procedure to produce GIA material in greenhouse conditions. The treatment of type II diabetes is sophisticated in terms of the variety of risk factors, one of which is the considerably rapid development of postprandial hyperglycemia [5]. Postprandial hyperglycemia occurs through the reaction of α-glucosidase and α-amylase in order to produce glucose. Therefore, by restricting or reducing the activity of these enzymes, the occurrence of postprandial hyperglycemia should decrease. The inhibition of α-glucosidase activity leads to a deterioration in disaccharide hydrolysis which has substantial long-term effects on glycemic index control in diabetic patients [6]. In recent years, natural medicines from plants have been used as useful treatments without any side effects.Hairy roots (HRs) are established from the interaction between Agrobacterium rhizogenes and the host plant (gene transformation from bacteria to the genome of the plant) [7]. There are numerous merits that accrue from the collaboration; in particular, a high growth rate without the dependence of any exogenous hormones, genetic stability, and a high branching rate. In addition, the ability to produce secondary metabolites remains stable. Hence, the HR cultural technique is considered to be an attractive substitute for the manufacture of tremendously valuable natural secondary metabolites instead of bud or callus cultures [8].Accompanied by the significant progress of modern techniques, aeroponics is a new method that has been adapted from classical hydroponics. Compared with hydropon...

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Elicitor-enhanced Silymarin production in hairy root cultures of Silybum marianum L. Gaertn

Cited by 9 publications

References 0 publications

Hydroponics and elicitation, a combined approach to enhance the production of designer secondary medicinal metabolites in Silybum marianum

Hydroponics and elicitation, a combined approach to enhance the production of designer secondary medicinal metabolites in Silybum marianum

Biosynthesis and Regulation of Antioxidant Flavonolignans in Milk Thistle

Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor

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