Estimation of Oxidative Stress and Survivorship in Drosophila

Nayak, Nibedita; Mishra, Monalisa

doi:10.1007/978-1-4939-9756-5_11

Cited by 8 publications

(10 citation statements)

References 39 publications

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“…An equal volume of 1.6 mM nitroblue tetrazolium (NBT) solution (11383213001, Sigma-Aldrich, Merck, Germany) was added to the mixture and left for 1 h in the dark. NBT (11383213001, Sigma-Aldrich, Merck, Germany) assay was performed on the hemolymph according to the protocols of Nayak et al 2020 and Bag et al 2020. , NBT (11383213001, Sigma-Aldrich, Merck, Germany) (1.6 M) solution was given to the hemolymph and left for 1 h in the dark. The reaction was stopped after 1 h by adding an equivalent amount of 100% glacial acetic acid (GAA) (A6283, Sigma-Aldrich, Merck, Germany) and incubating for 5 min.…”

Section: Methodsmentioning

confidence: 99%

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Kumar

Bauri

Sahu

et al. 2023

ACS Appl. Bio Mater.

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Nanocomposites have significantly contributed to biomedical science due to less aggregation behavior and enhanced physicochemical properties. This study synthesized a MnFe2O4@poly(tBGE-alt-PA) nanocomposite for the first time and physicochemically characterized it. The obtained hybrid nanomaterial was tested in vivo for its toxicological properties before use in drug delivery, tissue engineering fields, and environmental applications. The composite was biocompatible with mouse fibroblast cells and hemocompatible with 2% RBC suspension. This nanocomposite was tested on Drosophila melanogaster due to its small size, well-sequenced genome, and low cost of testing. The larvae’s crawling speed and direction were measured after feeding. No abnormal path and altered crawling pattern indicated the nonappearance of abnormal neurological disorder in the larva. The gut organ toxicity was further analyzed using DAPI and DCFH-DA dye to examine the structural anomalies. No apoptosis and necrosis were observed in the gut of the fruit fly. Next, adult flies were examined for phenotypic anomalies after their pupal phases emerged. No defects in the phenotypes, including the eye, wings, abdomen, and bristles, were found in our study. Based on these observations, the MnFe2O4@poly(tBGE-alt-PA) composite may be used for various biomedical and environmental applications.

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Section: Methodsmentioning

confidence: 99%

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Kumar

Bauri

Sahu

et al. 2023

ACS Appl. Bio Mater.

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“…The larvae from each treated group along with the control group were collected and rinsed with 1× phosphate-buffered saline (PBS) to eliminate the food residue. 25,34 The larvae's gut was dissected on a glass slide using fine forceps under a stereomicroscope (ACCU-SCOPE Inc., Commack, New York). The dissected guts were transferred to a 1.5 mL tube containing 4% PFA (paraformaldehyde) and further kept at 4 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Keeping this in view, in the present study, various tests will be performed to study the toxicological behavior of the ZnFe 2 O 4 @poly( t BGE- alt -PA) composite in a fruit fly model, viz ., Drosophila melanogaster . These tests will be carried out on genetic, cellular, morphological, and developmental levels as the fruit fly is a suitable model for studying NM/NP toxicity. − Based on the results, the nanocomposite will be tested further in a rodent model soon.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Toxicological Analysis of the ZnFe₂O₄@poly(tBGE-alt-PA) Nanocomposite: A Study on Fruit Fly

Chauhan,

Naik,

Kumar

et al. 2024

ACS Omega

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Recently, the use of hybrid nanomaterials (NMs)/nanocomposites has widely increased for the health, energy, and environment sectors due to their improved physicochemical properties and reduced aggregation behavior. However, prior to their use in such sectors, it is mandatory to study their toxicological behavior in detail. In the present study, a ZnFe 2 O 4 @poly(tBGE-alt-PA) nanocomposite is tested to study its toxicological effects on a fruit fly model. This nanocomposite was synthesized earlier by our group and physicochemically characterized using different techniques. In this study, various neurological, developmental, genotoxic, and morphological tests were carried out to investigate the toxic effects of nanocomposite on Drosophila melanogaster. As a result, an abnormal crawling speed of third instar larvae and a change in the climbing behavior of treated flies were observed, suggesting a neurological disorder in the fruit flies. DAPI and DCFH-DA dyes analyzed the abnormalities in the larva's gut of fruit flies. Furthermore, the deformities were also seen in the wings and eyes of the treated flies. These obtained results suggested that the ZnFe 2 O 4 @poly(tBGE-alt-PA) nanocomposite is toxic to fruit flies. Moreover, this is essential to analyze the toxicity of this hybrid NM again in a rodent model in the future.

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“…Based on this, we used EPR spectroscopy ( Elajaili et al, 2019 ) technique to precisely quantify ROS at the tissue level ( Berg et al, 2014 ); it is known in fact various exogenous factors like temperature, pressure, stress, can produce oxidative stress within the body and hence generates ROS ( Nayak and Mihra, 2019 ). Various exogenous factors like temperature, sound, pressure, microbe infection and chemicals can produce oxidative stress within the body.…”

Section: Methodsmentioning

confidence: 99%

Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure

et al. 2022

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During recent decades, model organisms such as Drosophila melanogaster have made it possible to study the effects of different environmental oxygen conditions on lifespan and oxidative stress. However, many studies have often yielded controversial results usually assigned to variations in Drosophila genetic background and differences in study design. In this study, we compared longevity and ROS levels in young, unmated males of three laboratory wild-type lines (Canton-S, Oregon-R and Berlin-K) and one mutant line (Sod1n1) as a positive control of redox imbalance, under both normoxic and hypoxic (2% oxygen - 24h) conditions. Lifespan was used to detect the effects of hypoxic treatment and differences were analysed by means of Kaplan-Meier survival curves and log-rank test. Electron paramagnetic resonance spectroscopy was used to measure ROS levels and analysis of variance was used to estimate the effects of hypoxic treatment and to assess ROS differences between strains. We observed that the genetic background is a relevant factor involved in Drosophila m. longevity and ROS levels. Indeed, as expected, in normoxia Sod1n1 are the shortest-lived, while the wild-type strains, despite a longer lifespan, show some differences, with the Canton-S line displaying the lowest mortality rate. After hypoxic stress these variances are amplified, with Berlin-K flies showing the highest mortality rate and most evident reduction of lifespan. Moreover, our analysis highlighted differential effects of hypoxia on redox balance/unbalance. Canton-S flies had the lowest increase of ROS level compared to all the other strains, confirming it to be the less sensitive to hypoxic stress. Sod1n1 flies displayed the highest ROS levels in normoxia and after hypoxia. These results should be used to further standardize future Drosophila research models designed to investigate genes and pathways that may be involved in lifespan and/or ROS, as well as comparative studies on specific mutant strains.

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Estimation of Oxidative Stress and Survivorship in Drosophila

Cited by 8 publications

References 39 publications

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of the ZnFe₂O₄@poly(tBGE-alt-PA) Nanocomposite: A Study on Fruit Fly

Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure

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Estimation of Oxidative Stress and Survivorship in Drosophila

Cited by 8 publications

References 39 publications

In Vivo Toxicological Analysis of MnFe2O4@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe2O4@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of the ZnFe2O4@poly(tBGE-alt-PA) Nanocomposite: A Study on Fruit Fly

Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure

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In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of the ZnFe₂O₄@poly(tBGE-alt-PA) Nanocomposite: A Study on Fruit Fly