Estrogenic Effect of Soy Phytoestrogens on the Uterus of Ovariectomized Female Rats

Helmy, Seham A.

doi:10.4172/2167-065x.s2-001

Cited by 4 publications

(6 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The remaining 20 females were subjected to ovariectomy via midline incision according to the method of Lasota and Danowska-Klonowska [38]. All rats were gavaged with amoxicillin (Amoun Pharmaceutical Co., Egypt) of 10 mg/kg body weight as Ibiamox® in the form of oral suspension for 3 days after surgical intervention [39].…”

Section: Methodsmentioning

confidence: 99%

“…Feed intake and body weight gain were recorded/week according to Helmy et al [39]. Cumulative feed intake and cumulative body weight gain were also calculated.…”

Section: Methodsmentioning

confidence: 99%

“…Formalin-fixed tibia were subjected to decalcification in 5% trichloroacetic acid for 20 days then dehydrated and stained with H&E. All histopathological procedures were performed according to Bancroft and Gamble [48]. Paraffin-embedded tibia were subjected to immunohistochemistry (IHC) using a primary antibody for ER α (Thermo Scientific Co., UK) according to the methodology of Helmy et al [39]. The percentages of the IHC-stained area (IHC area %) were obtained using ImageJ software according to Elgawish et al [49].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Soy Isoflavones Ameliorate Metabolic and Immunological Alterations of Ovariectomy in Female Wistar Rats: Antioxidant and Estrogen Sparing Potential

Abdelrazek

Mahmoud

Tag

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Hormone replacement therapy (HRT) can alleviate estrogen deficiency symptoms especially during menopause. The present study aimed at investigating the effect of soy isoflavones as HRT on immunological and bone health-related parameters with a special focus on the interactions between immunological status and metabolism. Thirty healthy cyclic female Wistar rats were used in this experiment. Ten females were sham-operated, and 20 females were subjected to ovariectomy. Overiectomized (OVX) female rats were randomly divided into 2 groups: the control group (G1, OVX/casein) was fed a casein-based diet, and the second group (G2, OVX/soy) was fed a high soy isoflavone diet. Both groups were compared to a sham-operated group (G3, sham/casein). Treatments continued for 7 weeks. Feed intake, weight gain, and lymphoid organ relative weights were recorded. Some metabolic, immunological, and bone health-related parameters were measured. Moreover, nitric oxide (NO), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined. Bone histopathology and immunohistochemistry to estrogen receptor alpha (ERα) were done. Feeding soy to OVX females reduced feed intake, weight gain, relative lymphoid organ weight, and T-lymphocytes transformation. Soy isoflavone administration normalized nearly all metabolic and immunological parameters to a level comparable to the sham group via oxidative stress amelioration and bone ERα promotion. Soy isoflavones seemed to be good HRT in estrogen deprivation which modulated the appetite, weight gain, lipid profile, proinflammation, and bone turnover.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Feed intake and body weight gain were recorded/week according to Helmy et al [39]. Cumulative feed intake and cumulative body weight gain were also calculated.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Soy Isoflavones Ameliorate Metabolic and Immunological Alterations of Ovariectomy in Female Wistar Rats: Antioxidant and Estrogen Sparing Potential

Abdelrazek

Mahmoud

Tag

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…From Table 1, it may be found that miceuterine weight is higher by giving Katu Leaf extract. Previous study by Helmy (2014) shows that the phytoestrogen intake can increase the endometrial thickness and then the uterine weight. This action is modulated by binding of the isoflavone and estrogen receptors.…”

Section: Discussionmentioning

confidence: 93%

Sauropus Androgynus for Increasing Uterine Weight in Menopausal Women: An Experimental Study Using Animal Models

Hikmah

Susilowati

2017

Proceedings of Surabaya International Physiology Seminar

View full text Add to dashboard Cite

Menopause is a natural phase of life and happens in every woman who reaches a certain age. This condition has many problems, such as endometrial atrophy, which can cause abnormal bleeding. Sauropus androgynus, known as Katu Leaf, has isoflavones compound and acts as an estrogen-like agent or phytoestrogen. Isoflavone can induce cell proliferation activity in increasing endometrial thickness and uterine weight. This study was aimed to find how the is flavone compound in Sauropus androgynus can increase the uterine weight in menopausal mice as an animal model. Twenty female mice aged two months and one week were given VCD (4-Vinyl cyclohexane dioxide) to induce the atresia acceleration of ovarian follicles, then treated with Katu Leaf extract of 0, 15 and 30mg/kgBW over four weeks. The uterine weights were measured and analyzed with One Way Analysis of Variances (Anova) and further tested with Least Significance Difference in 95% confidence interval. The result showed a difference between control and treatments group (p<0.05) with 30mg/kgBW as highest dosage, which increase uterine weight as 112.80 +8.86mg), but this is not higher than normal (116.36+6.83mg). This study suggests that Katu Leaf extract has an isoflavone compound which can increase uterine weight in menopausal mice.

show abstract

“…The sperm cells morphology of soymilk treated groups become abnormal and decreased number of leydig and sertoli cells [14]. The lumen of uterine become highly branched and increased the thickness of uterine wall due to glandular epithelium hyperplasia, increase blood supply and connective tissue edema as well as formed new blood vessels [15]. Soy isoflavone decreased in number of corpus luteum and increased atretic follicular count [16].…”

Section: Introductionmentioning

confidence: 99%

Effect of isoflavone (SOY) on histopathology of reproductive organs of rabbits (Oryctolagus cuniculus)

Batool¹

2020

PAB

View full text Add to dashboard Cite

The main source of isoflavones is soy foods. Soy isoflavones have been extensively studied for its antiestrogenic and estrogenic effects. These exogenous chemicals have less potent effect but there mechanism of action is same as endogenous estrogen. They have adverse effects on histology of testis and ovaries. In present study we evaluate the effect of soy isoflavone on histopathological changes in testis and ovaries. Selected 84 adult rabbits were divided into 8 groups. The dose groups were (2.5 mg/kg per body weight= A group), (3.5 mg/kg per body weight= B group), (5 mg/kg per body weight = C group) and control (D) group separately for male and female. The results showed that soy isoflavone have negative effect on reproductive organs and more pronounced effect on male than female. Many histological problems were observed in testis and ovaries. This study shows that soy foods are not good for reproductive health, and use of soy foods in high amount for prolonged time should be prohibited.

show abstract

Estrogenic Effect of Soy Phytoestrogens on the Uterus of Ovariectomized Female Rats

Cited by 4 publications

References 65 publications

Soy Isoflavones Ameliorate Metabolic and Immunological Alterations of Ovariectomy in Female Wistar Rats: Antioxidant and Estrogen Sparing Potential

Soy Isoflavones Ameliorate Metabolic and Immunological Alterations of Ovariectomy in Female Wistar Rats: Antioxidant and Estrogen Sparing Potential

Sauropus Androgynus for Increasing Uterine Weight in Menopausal Women: An Experimental Study Using Animal Models

Effect of isoflavone (SOY) on histopathology of reproductive organs of rabbits (Oryctolagus cuniculus)

Contact Info

Product

Resources

About