Evaluation of the Lipophilicity of Some Dehydroepiandrosterone Derivates Using RP-18 HPTLC Chromatography

Perišić-Janjić, Nada U.; Djaković‐Sekulić, Tatjana; Stojanović, Srdjan Z.; Penov-Gaši, Katarina M.

doi:10.1365/s10337-004-0252-6

Cited by 13 publications

(5 citation statements)

References 10 publications

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“…The scale of lipophilicity, based on the isocratic retention factors, has been preferred by some authors since it requires fewer experiments (Pyka and Bober, ). A variety of descriptors that can be used as lipophilicity estimators were provided by RP‐TLC technique (Pyka and Miszczyk, ; Musilek et al, ; Kerns and Di, ; Perisic‐Janjic et al, ; Kostecka et al, ). The retardation factor (R F ) values are averages from at least three chromatograms developed for each solute–mobile phase combination, and are calculated on the basis of migration distance of compound relative to the migration distance of the solvent front.…”

Section: Methodsmentioning

confidence: 99%

Lipophilicity study of eight cephalosporins by reversed‐phase thin‐layer chromatographic method

Dąbrowska

Komsta

Krzek

et al. 2015

Biomedical Chromatography

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The lipophilicity (R(M0)) and specific hydrophobic surface area for the representatives of four generation cephalosporins have been determined by reversed-phase thin-layer chromatography, and the effect of different mobile-phase modifiers (such as methanol, acetonitrile, acetone, 1,4-dioxane and 2-propanol) on the retention has been studied. The compounds studied showed typical retention behavior; their R(M) values decreased linearly with increasing concentration of the organic modifier in the eluent. The linear correlations between the volume fraction of the organic solvent and the R(M) values over a limited range were established for each solute, resulting in high values of correlation coefficients (>0.95 in most cases). R(M) values were determined by various concentrations of organic modifier, and the correlation obtained was extrapolated to 0% of organic modifier. Chromatographically established logP (R(M0)) parameters were compared with computationally calculated partition coefficients values (AClogP, ALOGP, KOWWIN, ALOGPs, XLOGP2, MLOGP and XLOGP3) and experimental octanol-water logP values (measured by the shake flask method). The received results demonstrate that RP-TLC may be a good alternative technique for analytics in describing the lipophilic nature of investigated cephalosporins as well as the activity.

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Section: Methodsmentioning

confidence: 99%

Lipophilicity study of eight cephalosporins by reversed‐phase thin‐layer chromatographic method

Dąbrowska

Komsta

Krzek

et al. 2015

Biomedical Chromatography

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“…They were chromatographed on C18 RPTLC stationary phase using acetonitrile-water mixture as mobile phase [83]. Perisic-Janjic et al evaluated the lipophilicity of some dehydroepiandrosterone derivatives by HPTLC [84]. In another study, the same group applied normal-phase TLC retention data in QSAR studies and determined the structure of dehydroepiandrosterone derivatives [85].…”

Section: Determination Of Lipophilicitymentioning

confidence: 99%

Analysis of anticancer drugs using thin layer chromatography- A review

Uğur¹,

Uğur²

2018

jrp

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ABSTRACT:Cancer is a fatal disease and cancer incidence is increasing from day to day. Thus, anticancer drugs are widely used and their analysis requires simple and effective analytical procedures. Thin layer chromatography (TLC) is a promising technique for drug analysis as a simple and versatile technique with low cost of analysis, minimal sample clean-up and high sample loading capacity. An extensive literature survey has been done and TLC techniques used for the analysis of anticancer agents in different matrices have been presented.

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“…There are many different ways for determination of DHEA. Some of the methods can be: reversed phase ion-pair high performance liquid chromatography, [10] liquid chromatography=mass spectrometry, [11] high performance liquid chromatography [12] or highperformance thin-layer chromatography, [13] and liquid chromatographytandem mass spectrometry. [14] The method used for DHEA investigation in this work was thin layer chromatography.…”

Section: Introductionmentioning

confidence: 99%

Densitometry Application for Evaluation of the Visualizing Agents for Dehydroepiandrosterone

Bober

2008

Journal of Liquid Chromatography & Related Technologies

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In our research the dehydroepiandrosterone (DHEA) was detected. The main aim of the work was to determine the possibility of application for the new visualizing agents for DHEA. Methylene violet, gentian violet, janus blue, methylene blue, and malachite green were used as visualizing agents. Chloramine T and rodamine B were used as comparative reagents described in literature.DHEA solution was spotted on chromatographic plates and then dipped in solutions of visualizing reagents, dried and scanned using a densitometer. The plates without using visualizing agents, as well as after using procedure described in literature (using chloramine T) were also scanned. The bands with maximum absorption (k max ) for DHEA without and with using visualizing agents were settled on the base of spectrodensitometric analysis. Consequently, the densitometric analysis of DHEA by (k max ) determined was carried out. On the base of concentration values of investigated solution of DHEA and height and areas of densitometric bands, the relationships between heights of densitomteric bands and concentrations of DHEA, as well as between areas of densitometric bands and concentrations of DHEA were settled. The values of detectability and range of quantification, as well as the angle between tangents at the inflection points to the curves of the densitometric peaks (b) were determined.

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Evaluation of the Lipophilicity of Some Dehydroepiandrosterone Derivates Using RP-18 HPTLC Chromatography

Cited by 13 publications

References 10 publications

Lipophilicity study of eight cephalosporins by reversed‐phase thin‐layer chromatographic method

Lipophilicity study of eight cephalosporins by reversed‐phase thin‐layer chromatographic method

Analysis of anticancer drugs using thin layer chromatography- A review

Densitometry Application for Evaluation of the Visualizing Agents for Dehydroepiandrosterone

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