Extraction of Phenolic Compounds and Antioxidant Activity from Cayenne Pepper fruit by Microwave Assisted Extraction

Kusnadi, Joni; Dedi, Dedi; Yunianta, Yunianta; Arumingtyas, Estri Laras

doi:10.21776/ub.jtp.2017.018.03.18

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Based on Figure 2, the highest average total phenolic in acetone solvent was at MAE 5 minutes of 133.8 mg EAG/g, then with the length of extraction time the total phenolic produced decreased. This is in line with research conducted by [13] which reported that the extraction of cayenne pepper experienced a decrease in the total phenolic value as the extraction time increased. This decrease is due to the presence of peroxidase enzymes outside the vacuole or inside the vacuole which diffuses into the organelles which causes oxidation during the extraction process.…”

Section: Effect Of Solvent and Time Variation On Total Phenolicsupporting

confidence: 92%

Total phenolic and flavonoid test of Gleichenia linearis leaf extract as antioxidant using microwave assisted extraction

Pratiwi,

Roanisca,

Nurhadini

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Excessive exposure to free radicals can cause oxidative stress, thus requiring defense mechanisms to prevent damage, one of which is the use of antioxidants. Resam (Gleichenia linearis ) leaf extract is known to contain flavonoids, alkaloids, tannins, saponins, steroids, and triterpenoids. This research aims to determine the influence of solvents and extraction time variations on the yield, total phenolic content, total flavonoid content, and antioxidant activity of resam leaf extract. The testing comprises the determination of total phenolic content using the Folin-Ciocalteau method, total flavonoid content using the AlCl3 method, and antioxidant activity using the DPPH method. The highest yield, total phenolic content, total flavonoid content, and antioxidant activity in this research were obtained from ethanol as the solvent with a MAE time of 10 minutes, with respective values of 62.21%, 172.9 mg EAG/g, 110.60 mg QE/g, and 24.12 µg/mL. The correlation testing showed that total phenolic content contributes 95.5%, while total flavonoid content contributes 56.5% to the generated antioxidant activity (IC50). Phenolic compounds are the most dominant compounds in the resam leaf extract, which play a role as antioxidants.

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Section: Effect Of Solvent and Time Variation On Total Phenolicsupporting

confidence: 92%

Total phenolic and flavonoid test of Gleichenia linearis leaf extract as antioxidant using microwave assisted extraction

Pratiwi,

Roanisca,

Nurhadini

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Therefore the concentration found was lacking. According to the research conducted by Kusnadi et al (2017), which used microwaveassisted extraction using a 1:15 ratio, the 1:15 ratio gave a lower concentration than that of the 1:10 ratio. This is in line with this study, where the total flavonoid concentration decreased after reaching the maximum ratio of 1:40.…”

Section: The Effect Of Extraction Time and The Solid To Solvent Ratio...mentioning

confidence: 99%

The Effect of Solvent Ratio and Extraction Time on Antioxidant Activity and Flavonoid Concentration of Kedawung Leaf (Parkia Biglobosa) Through Microwave-Assisted Extraction

Indrasari¹,

Buanasari²

2022

JBAT

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Kedawung (Parkia biglobosa) contains various ingredients such as alkaloids, saponins, tannins, flavonoids, and terpenoids. The flavonoid content in Kedawung is thought to have an antioxidant effect. Antioxidants are able to counteract free radicals that enter the body by donating electrons and binding them. Currently, the microwave-assisted extraction (MAE) method is widely used because the solute mass transfer from the sample matrix into the solvent is higher than the Soxhlet method. The following study aimed to know the effect of solvent ratio and extraction time on the extraction yield, flavonoid concentration, and antioxidant activity of kedawung leaf through microwave-assisted extraction. In this method, we used 40% ethanol to make the varied solute: solvent ratio such as 1:20, 1:30, 1:40, and 1:50. The extraction time used in this method was 4-7 minutes respectively. Microwave-assisted extraction has good performance to extract the active substance in Kedawung leaves. The highest yield 16.36%, total flavonoid content (57.32±2,2 mg QE/g extract), and DPPH scavenging activity (88.87±1.062%) was obtained in the extraction with a solids-solvent ratio of 1:40 g/mL, at an extraction time of 6 minutes. This method promises to take the active substance in a short time.

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“…Namun, dengan menggunakan metode ekstraksi konvensional mempunyai kelemahan yaitu dapat terjadinya kerusakan senyawa fenolik akibat reaksi oksidasi, hidrolisis dan bahkan ionisasi selama ekstraksi berlangsung (Kunarto & Sani, 2019;. Waktu ekstraksi dengan metode konvensional yang lama, juga dapat mengakibatkan rusaknya senyawa fenolik (Sari et al, 2011;Handayani et al, 2016;Kusnadi et al, 2017).…”

Section: Pendahuluanunclassified

Karakteristik Nanoemulsi Ekstrak Buah Parijoto (Medinilla speciosa Blume)

Siqhny

Azkia

Kunarto

2020

jtphp

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Parijoto (Medinilla speciosa) tumbuh subur dan tumbuh liar di lereng-lereng gunung atau di hutan-hutan pada tanah yang berhumus tinggi dan lembab di lereng gunung mulai pada ketinggian 700 hingga 2.300 meter diatas permukaan laut. Salah satu lokasi paling banyak ditemukan tanaman parijoto terdapat di lereng Pegunungan Muria, Desa Colo, Kecamatan Dawe, Kabupaten Kudus, Jawa Tengah. Buah parijoto mengandung senyawa fenolik dan flavonoid yang tinggi, diharapkan buah parijoto dapat digunakan sebagai alternatif pengganti antioksidan sintetik seperti Buttylated Hydroxyanisol (BHA) dan Butilated Hydroxytoluen (BHT). Senyawa BHA dan BHT sejak lama belum diterima sepenuhnya oleh konsumen karena dianggap berbahaya bagi kesehatan tubuh karena dianggap mengandung zat karsinogenik. Penggunaan buah parijoto sebagai antioksidan alami ke dalam makanan, minuman atau produk lainya dalam bentuk utuh maupun irisan sangat tidak efisien. Untuk itu perlu dilakukan tahap ekstraksi buah parijoto. Ultrasonic Assisted Extraction (UAE) adalah salah satu metode ektraksi dengan bantuan gelombang ultrasonik. metode ini adalah metode alternatif ekstraksi non-termal yang lebih efisien, lebih cepat, dan memungkinkan pengurangan pelarut, sehingga menghasilkan ekstrak murni dan yield yang lebih tinggi dibandingkan dengan ekstraksi konvesional. Untuk dapat meningkatkan efek yang optimum penggunaan ekstrak parijoto, diperlukan kondisi bahan yang baik, yaitu seperti bentuk sediaan nanoemulsi. Nanoemulsi merupakan partikel koloid padat dengan diameter 1-1000 nm. Dengan ukuran tersebut, globul-globul dapat terpenetrasi baik dan menembus lapisan pori bahan yang akan dicoating, sehingga ekstrak parijoto yang terlarut dalam globul akan banyak berpenetrasi. Sifat fisik dan stabilitas emulsi dipengaruhi oleh jenis dan konsentrasi emulgator. Jenis emulgator, Tween 80 dipilih dalam penelitian ini. Adanya emulgator yang ditambahkan akan menghasilkan monolayer yang mengelilingi droplet. Komposisi dari emulgator akan dikaji lebih lanjut, dimana Tween 80 yang memiliki nilai HLB 15 akan ditambahkan dengan beberapa variasi konsentrasi.

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Extraction of Phenolic Compounds and Antioxidant Activity from Cayenne Pepper fruit by Microwave Assisted Extraction

Cited by 4 publications

References 13 publications

Total phenolic and flavonoid test of Gleichenia linearis leaf extract as antioxidant using microwave assisted extraction

Total phenolic and flavonoid test of Gleichenia linearis leaf extract as antioxidant using microwave assisted extraction

The Effect of Solvent Ratio and Extraction Time on Antioxidant Activity and Flavonoid Concentration of Kedawung Leaf (Parkia Biglobosa) Through Microwave-Assisted Extraction

Karakteristik Nanoemulsi Ekstrak Buah Parijoto (Medinilla speciosa Blume)

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