Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor

Abstract

NKp46 is a cell surface receptor expressed on natural killer (NK) cells, on a minute subset of T cells, and on a population of innate lymphoid cells that produce

Conditional ablation of NKp46+ cells using a novel Ncr1greenCre mouse strain: NK cells are essential for protection against pulmonary B16 metastases

“…Ncr1 greenCre mice express an EGFPcre fusion transgene in NKp46 + cells from all organs. This mouse line differs from previously reported respective BAC-and Knock-in based NK-Cre mice [15,16] in that it uses only a short proximal promoter fragment derived from the C57BL/6 background directing the expression of an EGFPcre fusion to NKp46 + cells. The use of an EGFPcre fusion permits the direct detection of Cre-expressing NK cells by FACS or histological analyses.…”

“…In order to study gene functions specifically in NKp46 + cells, we and others [15,16] have created mice that allow for conditional gene targeting in Ncr1 expressing cells using the Cre-loxP system [17]. To this end, we generated Ncr1 greenCre mice expressing an EGFPcre fusion under the control of a proximal C57BL/6 Ncr1 promoter fragment.…”

“…Furthermore, studying the role of NK cells in immune responses often involves the use of genetargeted mice lacking NK cells that also harbor other lymphoid defects (e.g. Rag2 −/− Il2rg −/− mice [11] or IL-15 −/− mice [12]), or depletion of NK cells using antibodies that are not NK-cell specific, such as anti-NK1.1 (expressed by NKT cells) or anti-asialo-GM1 (expressed by myeloid cells) [13,14].In order to study gene functions specifically in NKp46 + cells, we and others [15,16] have created mice that allow for conditional gene targeting in Ncr1 expressing cells using the Cre-loxP system [17]. To this end, we generated Ncr1 greenCre mice expressing an EGFPcre fusion under the control of a proximal C57BL/6 Ncr1 promoter fragment.…”

Les cellules lymphoïdes innées

“…Les cellules NK et LTi sont restées les seuls types de lymphocytes innés connus jusqu'à ce qu'une dizaine d'équipes révèlent simultanément l'existence de tout un pan de l'immunologie jusqu'alors inconnu. Une série d'articles, parus entre 2008 et 2011, montre en effet l'existence de nouvelles cellules lymphocytaires innées alors dénommées, entre autres, NK22, ROR(retinoic acid receptor related orphan receptor)t + NKp46 + , NK-LTi, nuocytes ou encore natural helper cells [6][7][8][9][10][11][12]. Très rapidement, en recoupant leurs caractéris-tiques, ces cellules nouvellement identifiées ont été classées en trois catégories : les ILC de type 1 (dont font partie les cellules NK), les ILC de type 2 et les ILC de type 3 (dont font partie les LTi) [13].…”

NKp46‐mediated Dicer1 inactivation results in defective NK‐cell differentiation and effector functions in mice

“…Moreover, in both studies, the extent of Dicer1 deletion was limited and as much as 50% of the initial microRNA quantity was still present. To clarify the role of Dicer1 in NK cells, we generated a model of NK-specific Dicer1 deletion by crossing Dicer1 lox/lox mice [9] with Ncr1 iCre mice expressing the improved Cre recombinase under the dependence of the Ncr1 promoter [22]. …”