Fish IRF3 up-regulates the transcriptional level of IRF1, IRF2, IRF3 and IRF7 in CIK cells

Xu, Xiaowen; Lai, Qinan; Gu, Meihui; Liú, Dan; Hou, Qunhao; Liu, Xiancheng; Mi, Yichuan; Sun, Zhicheng; Wang, Haizhou; Lin, Gang; Hu, Chengyu

doi:10.1016/j.fsi.2015.10.039

Cited by 13 publications

(5 citation statements)

References 53 publications

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“…As an example, both fish and mammalian IRF1 are positive activators of IFN, and both fish and mammalian IRF2 are negative regulators [35,36]. However, there are still several differences.…”

Section: Discussionmentioning

confidence: 99%

Fish IRF6 is a positive regulator of IFN expression and involved in both of the MyD88 and TBK1 pathways

Wang

et al. 2016

Fish & Shellfish Immunology

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“…As an example, both fish and mammalian IRF1 are positive activators of IFN, and both fish and mammalian IRF2 are negative regulators [35,36]. However, there are still several differences.…”

Section: Discussionmentioning

confidence: 99%

Fish IRF6 is a positive regulator of IFN expression and involved in both of the MyD88 and TBK1 pathways

Wang

et al. 2016

Fish & Shellfish Immunology

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“…Upon translocation to the nucleus, IRF3 and NF-κB initiate transcription, resulting in the activation of the classical JAK-STAT pathway and interferon-stimulating gene factor (ISGF)-3 (Tamura et al, 2008;Wang et al, 2016). Currently, fish IRF3 has been reported in seabass (Lates calcarifer), Grass carp (Ctenopharyngodon idella), rainbow trout (Oncorhynchus Mykiss) and Red sea Bream (Pagrus major), but the results are limited (Holland et al, 2008;Xu et al, 2015;Krishnan et al, 2019;Kim et al, 2020). Therefore, in this study, the expression of IRF3 in olive flounder tissue was analyzed in different developmental stages.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Tissue-Specific Interferon Regulatory Factor 3 (IRF3) Gene Expression against Viral Infection in Paralichthys olivaceus

Kim¹,

Lee²,

Park³

et al. 2021

Dev. Reprod.

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Interferon Regulatory Factor 3 (IRF3) is a member of interferon-regulated transcription factor family and is known to play an important role in the innate immune response against viral infections. In this study, the expression of IRF3 in different tissues, developmental stages, and stocking densities of olive flounder was investigated. The expression of IRF3 was observed to gradually increase in early-stage juvenile fish. The highest expression was observed in later-stage juvenile fish when immune tissues were formed. High IRF3 expression was observed in the muscles and the brain tissues. The expression of IRF3 was observed in fish at different stocking densities after viral hemorrhagic septicemia virus (VHSV) infection. It yielded an interesting expression pattern in the muscles and the brain tissues of fish stocked at low density. These observations can be used as basic data for the study of the expression of immune response-related genes against viruses based on stocking density and immune systems in other fish species.

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“…There are many feedback and cascade-amplification mechanisms in fish IFN signaling pathways (36). Analogously, our previous study indicated that grass carp IRF3 also has some similar mechanisms (37).…”

mentioning

confidence: 62%

“…Transfection, RNA interference-mediated gene-knockdown assays, and luciferase activity assays Each plasmid was transfected according to the FuGENE6 protocol. Stimulation and luciferase activity assays were carried out as described previously (37). In the RNA interference (RNAi)-mediated gene-knockdown assay, the specific small interfering RNA (siRNA) sequence against CiIRF3, CiSTING, and CiZDHHC1 and the negative control RNA oligonucleotides were designed and synthesized by Shanghai GenePharma (Table I), and the protocol of transfection was followed in the same manner as previously described (38).…”

Section: Plasmidsmentioning

confidence: 99%

Endoplasmic Reticulum Transmembrane Proteins ZDHHC1 and STING Both Act as Direct Adaptors for IRF3 Activation in Teleost

et al. 2017

The Journal of Immunology

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IFN regulatory factor (IRF)3 is a central regulator for IFN-β expression in different types of pathogenic infections. Mammals have various pathogenic sensors that are involved in monitoring pathogen intrusions. These sensors can trigger IRF3-mediated antiviral responses through different pathways. Endoplasmic reticulum-associated proteins stimulator of IFN gene (STING) and zinc finger DHHC-type containing 1 (ZDHHC1) are critical mediators of IRF3 activation in response to viral DNA infections. In this study, grass carp STING and ZDHHC1 were found to have some similar molecular features and subcellular localization, and both were upregulated upon stimulation with polyinosinic:polycytidylic acid, B-DNA, or Z-DNA. Based on these results, we suggest that grass carp STING and ZDHHC1 might possess some properties similar to their mammalian counterparts. Overexpression of ZDHHC1 and STING in kidney cells upregulated IFN expression, whereas knockdown of IRF3 inhibited IFN activation. In addition, coimmunoprecipitation and GST pull-down assays demonstrated that STING and ZDHHC1 can interact separately with IRF3 and promote the dimerization and nuclear translocation of IRF3. Furthermore, we also found that small interfering RNA-mediated knockdown of STING could inhibit the expression of IFN and ZDHHC1 in fish cells. Similarly, knockdown of STING resulted in inhibition of the IFN promoter. In contrast, ZDHHC1 knockdown also inhibited IFN expression but had no apparent effect on STING, which indicates that STING is necessary for IFN activation through ZDHHC1. In conclusion, STING and ZDHHC1 in fish can respond to viral DNA or RNA molecules in cytoplasm, as well as activate IRF3 and, eventually, trigger IFN expression.

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Fish IRF3 up-regulates the transcriptional level of IRF1, IRF2, IRF3 and IRF7 in CIK cells

Cited by 13 publications

References 53 publications

Fish IRF6 is a positive regulator of IFN expression and involved in both of the MyD88 and TBK1 pathways

Fish IRF6 is a positive regulator of IFN expression and involved in both of the MyD88 and TBK1 pathways

Analysis of Tissue-Specific Interferon Regulatory Factor 3 (IRF3) Gene Expression against Viral Infection in Paralichthys olivaceus

Endoplasmic Reticulum Transmembrane Proteins ZDHHC1 and STING Both Act as Direct Adaptors for IRF3 Activation in Teleost

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