Flavobacterium lacicola sp. nov., isolated from a freshwater lake

Abstract

A bacterial strain, designated IMCC25901, was isolated from a freshwater lake, Soyang, in the Republic of Korea. The strain was Gram-stain-negative, aerobic, non-motile, orange-coloured and short rod-shaped. The 16S rRNA gene sequence analysis showed that strain IMCC25901 was most closely related to Flavobacterium yonginense HMD1001 (97.0 %) and formed a robust phylogenetic clade with other species of the genus Flavobacterium. Growth of strain IMCC25901 was observed at 10-30 °C (optimum, 20 °C), pH 6-8 (optimum, pH 7) and 0-1.0 % NaCl (optimum, 0 %). The DNA G+C content of strain IMCC25901 was 34.2 mol%. The major fatty acid constituents of the strain were anteiso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C15 : 0. Cells of strain IMCC25901 contained phosphatidylethanolamine, an unidentified phospholipid, two unidentified aminolipids and two unidentified lipids. The isoprenoid quinone detected in the strain was MK-6. On the basis of the taxonomic data obtained in this study, it was concluded that strain IMCC25901 represented a novel species in the genus Flavobacterium, for which the name Flavobacterium lacicola sp. nov. is proposed. The type strain of Flavobacterium lacicola is IMCC25901 (=KCTC 52571=NBRC 112883).

Flavobacterium franklandianum sp. nov. and Flavobacterium gawalongense sp. nov., isolated from glaciers on the Tibetan Plateau

“… Flavobacterium contain menaquinone-6 as a major respiratory quinone and phosphatidylethanolamine as a major polar lipid [4]. Flavobacterium strains widely inhabit various environments including soil [5, 6], animals [7, 8], Antarctic habitats [9, 10], glaciers [11, 12], plants [13] and water [14–16]. Flavobacterium is one of the most abundant bacterial genera in the cryosphere and a large number of species with validly published names have been found in Antarctic glaciers, such as Flavobacterium antarcticum [10], Flavobacterium laiguense [11], Flavobacterium noncentrifugens [17] Flavobacterium sinopsychrotolerans [18], Flavobacterium tiangeerense [19], Flavobacterium xueshanense [20] and Flavobacterium urumqiense [20].…”

Microbiomes of freshwater lake ecosystems

“…A huge number of novel bacteria belonging to various classes and families have been reported from freshwater lake ecosystem worldwide e.g. Ferribacterium limneticum, CdA-1 Coeur d'Alene Lake, 54 Hymenobacter aquatilis, HMF3095 T Artificial lake, 55 Sphingobium fontiphilum, Chen16-4 T Chengcing Lake, 56 Limnobacter thiooxidans, CS-K1 Chiemsee Lake, 57 Desulfovibrio idahonensis, CY1 T Coeur d'Alene, 58 Thiobaca trueperi, Eutrophic lake, 59 Listeria marthii, FSL S4-120 T Finger Lakes 60 Sphingomonas hengshuiensis, WHSC-8 T Hengshui Lake, 61 Kinneretia asaccharophila, KIN192 T Kinneret Lake, 62 Algoriphagus aquatilis A8-7 T Longhu Lake, 63 Limnohabitans curvus, MWH-C5 T Mondsee Lake, 64 Cloacibacterium rupense, R2A-16 T Rupa Lake, 65 Undibacterium seohonense, SHS5-24 T Seoho lake, 66 Polynucleobacter difficilis, AM-8B5 T Sevan Lake, 67 Flavobacterium chuncheonense Soyang Lake, 68 Flavobacterium soyangense, IMCC26223 T Soyang Lake, 69 Flavobacterium luteum IMCC26026 T, Soyang Lake, 68 Mucilaginibacter soyangensis, HME6664 T Soyang Lake, 70 Flavobacterium lacicola, IMCC25901 T Soyang lake, 71 Flavobacterium saliperosum, S13 T, Taihu Lake, 72 Roseomonas lacus TH-G33 T Taihu Lake, 73 Rhodoluna lacicola, MWH-Ta8 T Taihu Lake, 74 Nocardioides taihuensis, X17 T Taihu Lake, 75 Lysobacter oligotrophicus, 107-E2 T Tanago Ike, 76 and Nocardioides ungokensis, UKS-03 T Ungok Lake. 77…”