Free radicals related effects and antioxidants in saliva and serum of adolescents with Type 1 diabetes mellitus

Reznick, Abraham Z.; Shehadeh, Naim; Shafir, Yair; Nagler, Rafael M.

doi:10.1016/j.archoralbio.2006.02.004

Cited by 92 publications

(69 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, the salivary protein thiols were significantly decreased in the oral cancer patients when compared to healthy controls. Similar reports were available in studies on salivary and serum antioxidants levels (peroxidase, superoxide dismutase, salivary total antioxidant status) in type 1 diabetes mellitus patients where altered antioxidant levels were observed indicating oxidative stress [25].…”

Section: Discussionsupporting

confidence: 55%

Protein Thiols and Butryrylcholinestrase in Saliva of Oral Cancer Patients

et al. 2013

View full text Add to dashboard Cite

Oral squamous cell carcinoma is one of the most common malignancies recognized. Biomarkers which can predict presence of cancer and its progression can help in better management of these disorders. Over production of lipid peroxidation byproducts and disturbances in antioxidant defense system have been implicated in the pathogenesis of several diseases including oral cancer. Studies have shown a correlation of butyrylcholinesterase (BChE), with tumourigenesis, cell proliferation and cell differentiation. Earlier we have observed a significant elevation in plasma BChE and protein thiols in oral cancer patients which correlated well with stages of cancer. As it was not clear whether the above markers will be altered in saliva of oral cancer patients this study was undertaken. Institutional Ethics Committee gave permission to carry out this study. Total of 55 subjects comprising healthy controls (n = 30) and biopsy proven oral cancer patients (n = 25) consented to participate in this study. Salivary samples from cases were taken before any definitive treatment. Protein thiols and BChE were estimated in salivary samples using validated assay methods. Oral cancer patients had a significant increase in pre-treatment salivary BChE levels (p B 0.001) and a significant decrease (p B 0.001) in salivary thiols as compared to respective values in controls. Salivary protein thiols and BChE may have a role in pathophysiology of oral cancer. Saliva can be used as a potential non-invasive screening tool in oral cancer patients.

show abstract

Section: Discussionsupporting

confidence: 55%

Protein Thiols and Butryrylcholinestrase in Saliva of Oral Cancer Patients

et al. 2013

View full text Add to dashboard Cite

show abstract

“…As a result, glycosylation products attach to cells' receptors, triggering inflammation that stimulates immune cells to release cytokines [20]. Several experimental menopause and diabetes models have shown that the development of metabolic disorders causes some degeneration in the salivary glands [21,22]. Hypoestrogenic and hyperglycaemic effects cause the loss of the balance in the body's defence system that induces oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Effects of Diabetes on Post-Menopausal Rat Submandibular Glands: A Histopathological and Stereological Examination

et al. 2015

View full text Add to dashboard Cite

Objective: The menopause in elderly women is a physiological process where ovarian and uterine cycles end. Diabetes means higher blood glucose level that is a metabolic disease and has an increased incidence. The aim of the study was to examine the single or combined effects of menopause and diabetes that causes pathophysiological processes on submandibular gland on ovariectomy and diabetes induced rat models. Materials and Methods:Sprague Dawley twelve weeks old female (n=24) rats were divided randomly into four groups; Healthy control group (n=6), diabetic group (DM, n=6), ovariectomized group (OVX, n=6), post ovariectomy diabetes induced group (DM+OVX, n=6) individually. Histopathological, histochemical and stereological analyses were done in these groups.Results: Significant neutrophil cell infiltrations and myoepithelial cell proliferations, granular duct and seromucous acini damages and changes in the content of especially seromucous acini secretion in DM and/or OVX groups and distinctive interstitial and striated duct damages in post ovariectomy diabetes induced group were detected. Alterations ingranular ducts hypertrophic and in seromucous acini atrophic were determined in DM and/or OVX groups. Conclusion:The results revealed the pathophysiological processes that lead to morphological and functional alterations on the cellular level in submandibular glands. The molecular mechanisms related with pathogenesis of diabetes and menopause need further investigation.Keywords: Diabetes, gland, menopause, rat, submandibular ÖzetAmaç: İlerleyen yaş ile birlikte, menopoz, kadınlarda gözlenen overial ve menstrual sikluslarda azalma veya bitiş ile karakterize fizyolojik bir süreç ve diyabet ise insidansı artan yüksek kan şekeri seviyesi ile karakterize metabolik hastalıktır. Overektomi ve diyabet indüklenen sıçan modelleri submandibular bezi üzerinde menopozun ve diyabetin tek veya kombine etkilerinin neden olduğu patofizyolojik süreçle-ri incelemek amacı ile çalışma tasarlandı. Gereç ve Yöntem:Sprague Dawley on iki haftalık dişi (n=24) sıçanlar dört gruba randomize bir şekilde rastgele ayrıldı; Sağlıklı Kontrol Grubu (n=6), Diyabetik grup (n=6), Overektomize grup (n=6), Post Overektomize Diyabet İndüklenen Grup (n=6). Sonuçlar histopatolojik, histokimyasal ve stereolojik analizler ile değerlendirildi. Bulgular:Anlamlı nötrofil infiltrasyonları ve myoepitel hücre proliferasyonları, granüler kanal ve seromuköz hücre dejenerasyonları ve özellikle seromuköz asinusların salgısı içeriğindeki değişimler DM ve/veya OVX gruplarında, belirgin intersistiyel ödem ve çizgili kanal dejenerasyonları diyabet indüklenen post overektomize grup submandibular dokularında izlendi. Granular kanallarda hipertrofik değişiklikler, seromuköz asinuslarda atrofik değişiklikler DM ve/veya OVX gruplarında tespit edildi.Sonuç: Diyabet ve overektominin submandibular bezlerinde yol açtığı patofizyolojik süreçlere bağlı hücresel düzeyde morfolojik ve fonksiyonel değişiklikler gösterildi. Diyabet ve menopozun patogenezi ile ilişkili moleküle...

show abstract

“…periodontitis) and systemic (e.g. diabetes, inflammatory bowel disease) conditions (Arana et al 2006;Bahar et al 2007;Celec et al 2005;Gorelik et al 2007;Jahanshahi et al 2004;Kolarzyk et al 2006;Mashayekhi et al 2005;Nagler et al 2000;Reznick et al 2006;Ryo et al 2006;Sawamoto et al 2005;Sculley and Langley-Evans 2003;Sugano et al 2003;Takane et al 2002;Tsai et al 2005). Changes in salivary cortisol secretion patterns (Fiocco et al 2006;Giubilei et al 2001;Hatfield et al 2004;Porter et al 2002;Scherder et al 2006;Wolf et al 2002;Woods and Dimond 2002), amylase activity (Sramek et al 1995), and acetylcholinesterase activity (Sayer et al 2004) have been documented in persons with AD or MCI, attesting to the plausibility of exploiting saliva for neurodegenerative disease biomarker discovery.…”

Section: Introductionmentioning

confidence: 99%

Diurnal variations in salivary protein carbonyl levels in normal and cognitively impaired human subjects

Gornitsky

Geng

et al. 2008

AGE

View full text Add to dashboard Cite

Oxidative stress has been documented in tissues and biofluids of subjects with sporadic Alzheimer disease (AD) and mild cognitive impairment (MCI). The aim of this study was to determine whether (a) salivary protein carbonyls are elevated in AD and MCI subjects, (b) salivary protein carbonyl contents in these groups exhibit diurnal variation, and (c) apolipoprotein E ɛ4 (apoE ɛ4) carrier status impacts salivary carbonyl concentrations or rhythmicity in the AD and MCI cohorts. Unstimulated saliva was collected at fixed intervals between 8 AM and 10 PM from 15 AD subject , 21 MCI subjects, and 30 cognitively-intact controls. Salivary protein carbonyl concentrations were measured by ELISA. ApoE genotyping was performed on the AD and MCI individuals. For all groups, mean protein carbonyl contents were significantly elevated at 2 PM relative to other time points surveyed. Mean salivary protein carbonyl concentrations did not differ among the diagnostic groups. ApoE ɛ4 carriers exhibited less temporal variation in salivary protein carbonyls relative to noncarriers. Thus, protein carbonyl content exhibits diurnal variation in adult human saliva. ApoE ɛ4 carrier status may impact oropharyngeal disease expression by attenuating the inherent diurnal variability in salivary redox homeostasis. Salivary protein carbonyls do not differentiate AD and MCI from normal individuals. In conclusion, oxidative stress has been documented in tissues and biofluids of subjects with sporadic AD and MCI. This article demonstrates that levels of protein carbonyls, a marker of oxidative stress, exhibit robust diurnal variation in the saliva of normal elderly, MCI, and AD subjects. Apolipoprotein E ɛ4 allele carrier status may attenuate this temporal variability in salivary redox homeostasis and thereby impact the natural history of oropharyngeal diseases.

show abstract

Free radicals related effects and antioxidants in saliva and serum of adolescents with Type 1 diabetes mellitus

Cited by 92 publications

References 35 publications

Protein Thiols and Butryrylcholinestrase in Saliva of Oral Cancer Patients

Protein Thiols and Butryrylcholinestrase in Saliva of Oral Cancer Patients

Effects of Diabetes on Post-Menopausal Rat Submandibular Glands: A Histopathological and Stereological Examination

Diurnal variations in salivary protein carbonyl levels in normal and cognitively impaired human subjects

Contact Info

Product

Resources

About