General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin

Towards a New Reference Test for Surra in Camels

“…RoTat 1.2 is a predominant VSG expressed by most strains of T. evansi, and therefore it is being used as the major antigen in several serological diagnostic tests (1,23). The CATT/T.…”

“…Lysis of the trypanosomes occurs when RoTat 1.2-specific antibodies are present in the test serum. The TL assay is considered the gold standard for the presence of specific antibodies against African trypanosomes (21,23).…”

Development of ELISA Exploring Recombinant Variable Surface Glycoprotein for Diagnosis of Surra in Animals

“…Moreover, the number of genes of trypanosomes has been expressed in different host systems for several purposes. Earlier studies also report the significance of VSGs in the diagnosis of sleeping sickness and surra 26,36 . The whole cell lysates of T. evansi lead to strong cross-reactions with T. vivax, T. congolense and even T. cruzi 37 .…”

“…Moreover, T. evansi infection in humans has been reported from Asian countries such as India and Sri Lanka 32,33 . VSG is expressed at early, middle and later stages of infection 26 . Moreover, the VSG antigenicity elicits sufficient level of antibody production in the host against the parasite 34 .…”

Surra Sero K-SeT, a new immunochromatographic test for serodiagnosis of Trypanosoma evansi infection in domestic animals

“…As a result, trypanosomes that are recognised by VSG-specific antibodies are distroyed (Horn, 2014;Pays et al, 2004). The VSG conferring the variant antigen type RoTat 1.2 is shared among most T. evansi strains, except in some rare T. evansi strains isolated from dromedary camels in Kenya (Ngaira et al, 2005;Verloo et al, 2001). Several antibody detection tests have been developed that http://dx.doi.org/10.1016/j.vetpar.2015.05.008 0304-4017/© 2015 Elsevier B.V. All rights reserved.…”