Genetic diversity and structure of native Egyptian cattle populations and French-Egyptian Cross via DNA-microsatellite

Abstract

This study investigates genetic diversity and structure of native Egyptian cattle populations, called Baladi, as Saidi from Southern Egypt, Menufi from Mid-Delta and their F1 crosses with the French Tarentaise breed using DNA Microsatellite markers. A total of unrelated 97 individuals were genotyped utilizing eight SSR primers (ETH10, ETH225, BM1818, BM1824, BM2113, SPS115, TGLA53 and TGLA126). All utilized SSR were found to be polymorphic. The highest and lowest numbers of alleles detected were 16 and 6 at TGLA53 and SPS115 loci, respectively. Baladi-Tarentaise crosses (Bal-Tar) had the highest number of alleles over all. The PIC values of 7 loci were higher than 0.5, indicating high allelic variation of utilized markers. Estimated PIC values were up to 0.898, 0.866 and 0.873 for TGLA53 genotyped in Saidi, Menufi and Bal-Tar, respectively. Hobs values were lower than the expected ones in the native populations accompanied with positive values for Fis and significant deviation from HWE indicating inbreeding trend in native populations. Structure analysis indicated three ancestral genetic backgrounds. The native populations share two main backgrounds in almost equal percentages, while the Bal-Tar had the third one. The three populations showed low percentage of admixture. The studied Mediterranean cattle populations that belong to Egypt and France seem to have differentiated from each other with only little genetic exchange between the geographically isolated populations so local cattle is very similar.

Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle

“…They are reared throughout the country, acclimatized to Egyptian conditions, and have a high tolerance to endemic diseases. Due to the low milk productivity of these animals, genetic improvement schemes have involved crossbreeding with exotic high producing cattle breeds such as Holstein, Brown Swiss, and Simmental ( 20 – 22 ), resulting in a large scattered population raised in small- or medium-sized herds by local farmers under the breed name “Mixed,” as described and characterized by the Ministry of Agriculture and Land Reclamation. Egyptian livestock import has increased over the last 10 years, improving the dairy industry performance.…”

Genetic Diversity Analysis and Determination of Specific Alleles of Kuantan Cattle Using Microsatellite Markers

“…Genetic diversities based on microsatellite markers in indigenous and local cattle have been reported for many breeds of cattle, such as West African cattle (Alvarez et al, 2021), East Eurasian cattle (Svishcheva, 2020); Russian cattle (Abdelmanova et al, 2020), some of Indian local cattle (Vinod et al, 2019); European cattle (Garkovenko et al, 2018), Korean native cattle (Sharma et al, 2020), Egyptian native cattle (Faid-Allah et al, 2018), Lebedyn cattle (Ladyka et al, 2019), Zimbabwean Sanga cattle (Gororo et al, 2018), South African cattle (Westhuizen et al, 2020); and Turkey cattle (Demir et al, 2019). The technique has been applied to characterize Indonesia's local breed animals such as Aceh cattle (Abdullah et al, 2011), Indonesian swamp local buffalo (Saputra et al, 2020), Bali cattle (Jakaria et al, 2012), and local Indonesian cattle (Sutarno et al, 2016;Agung et al, 2019;).…”

Application of Microsatellite Markers for Genetic Diversity Analysis of Indonesian Local Cattle

“…Marker mikrosatelit juga banyak digunakan untuk studi keragaman genetik populasi sapi di luar negeri, termasuk populasi sapi di Mesir (sapi Baladi dan sapi Menufi; lokus ETH10, ETH225, BM1818, BM1824, BM2113, SPS115, TGLA53 dan TGLA126), sapi di Eropa (sapi Tagil, sapi Red Gorbatov, dan sapi Ukrainan Grey; lokus ILST006, SPS115, BM1824, TGLA122, ETH3, ETH225, BM2113, TGLA126, CSSM66, TGLA53, INRA023, CSRM60, ETH10, dan TGLA227), sapi di Rusia (sapi Black Pied; lokus BM1818, BM1824, BM2113, ETH3, ETH10, ETH225, TGLA122, TGLA126, TGLA227, INRA023, dan SPS115), dan sapi di Spanyol (sapi Pirenaica; 30 lokus sesuai rekomendasi FAO) (Aitnazarov et al 2022;Faid-Allah et al 2018;Gamarra et al 2020;Svishcheva et al 2020). Hasil-hasil penelitian menunjukkan bahwa penggunaan marker mikrosatelit bermanfaat dalam memberikan informasi tentang level silang dalam, status keragaman genetik, level heterozygote deficiency/excess, serta struktur dan perbedaan genetik antar populasi.…”