Holographic tomography with object rotation and two-directional off-axis illumination

Abstract

A hybrid system of holographic tomography, which utilizes rotation of a sample and two-directional, off-axis illumination is proposed. The applied type of illumination brings two major benefits. First, it offers theoretical potential for the resolution improvement with respect to conventional tomography. Second, it enables effective, numerical compensation of the defocus error, which is achieved with an accurate, noise-immune autofocusing. Hence, the main practical obstacle of hybrid tomography is removed and its high-resolution potential is put into practice. The utility of the proposed concept is experimentally demonstrated with the tomographic measurement of a photonic crystal fiber.

Roadmap on Digital Holography-Based Quantitative Phase Imaging

“…For off-axis holography, this can be achieved by eliminating the zero order, autocorrelation, and conjugate image [145]. Further progress in multi-scale HT is aimed at resolution enhancement through filling the frequency support of the object more efficiently, e.g., by combining sample rotation and illumination scanning [146,147] or counteracting the missing cone artifact [148].…”

Is multiplexed off-axis holography for quantitative phase imaging more spatial bandwidth-efficient than on-axis holography? [Invited]

“…In optical multiplexing [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30], multiple sample and reference beam pairs with different 𝜃 𝑥 and 𝜃 𝑦 combinations are projected onto the digital camera simultaneously, each of which creating an off-axis hologram with a different interference fringe direction that positions one wavefront in the SFD without overlapping other terms. The simultaneous projection of all beams on the camera may create unwanted interference between nonmatching pairs.…”

“…Various basic architectures have been demonstrated for optical multiplexing. These include multiplexing two holograms by positioning two complex wavefronts in two orthogonal directions [11][12][13][14][15][16][17][18][19][20][21], which can be generalized to multiplexing three [22][23][24][25], four [26][27][28], five [29], or even six holograms [30], all without SFD overlap.…”

“…Tian et al multiplexed four low-resolution LED illuminations for Fourier Ptychography [28]. Finally, Dardikman et al multiplexed two angular views of the same scene over time to enable 4-D phase unwrapping [20], and Kostencka et al multiplexed two angular views for reducing the data acquisition time in tomographic phase microscopy [21].…”

Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution

“…In beam rotation tomography, the aforementioned spatial frequency collection constraint is commonly referred to as the missing cone problem 17 ; it results in a low axial resolution. In the sample rotation tomography method, the axial resolution can be improved by rotating the sample 19 – 24 , the sample is loaded into microcapillary or micropipette and rotated mechanically 4 , 24 – 27 . Consequently, achieving a full rotation of the specimen is difficult, and mechanical rotation makes the system susceptible to aberrations 23 – 25 .…”