The research presented herein aims to pinpoint the factors that cause errors in the order picking process in an e-commerce enterprise and to confirm or deny the assumption that these factors largely concern employees and their working conditions. Research method: A case study strategy, observation (non-compliance cards and identification of working conditions), and Lorenz–Pareto and Ishikawa were the methods used. Results: The research results indicate that working conditions are a key factor influencing the number of errors in the picking process. The usefulness of the research: The results show that paying particular attention to working conditions is not only good in the context of caring for the well-being of employees, but also as a means to reduce the number of errors and improve the pace of work. Limitations: Due to the methods used, which in turn result from the large variety of e-commerce entities, the research results are not universal and are limited to a specific situational context.