Hydrogen gas promotes the adventitious rooting in cucumber under cadmium stress

Wang, Bo; Bian, Biting; Wang, Chunlei; Li, Changxia; Fang, Hua; Zhang, Jing; Huang, Dengjing; Huo, Jianqiang; Liao, Weibiao

doi:10.1371/journal.pone.0212639

Cited by 22 publications

(20 citation statements)

References 48 publications

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“…We adapted a method from Wang et al (2019) for measuring the malondialdehyde (MDA) content. Leaves of ice plants were harvested before and after salt treatment, and then ground to a fine powder in liquid nitrogen.…”

Section: Malondialdehyde Content Measurementmentioning

confidence: 99%

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

et al. 2020

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Salt stress impedes plant growth and development, and leads to yield loss. Recently, a halophyte species Mesembryanthemum crystallinum has become a model to study plant photosynthetic responses to salt stress. It has an adaptive mechanism of shifting from C 3 photosynthesis to crassulacean acid metabolism (CAM) photosynthesis under stresses, which greatly enhances water usage efficiency and stress tolerance. In this study, we focused on investigating the morphological and physiological changes [e.g., leaf area, stomatal movement behavior, gas exchange, leaf succulence, and relative water content (RWC)] of M. crystallinum during the C 3 to CAM photosynthetic transition under salt stress. Our results showed that in M. crystallinum seedlings, CAM photosynthesis was initiated after 6 days of salt treatment, the transition takes place within a 3-day period, and plants became mostly CAM in 2 weeks. This result defined the transition period of a facultative CAM plant, laid a foundation for future studies on identifying the molecular switches responsible for the transition from C 3 to CAM, and contributed to the ultimate goal of engineering CAM characteristics into C 3 crops.

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Section: Malondialdehyde Content Measurementmentioning

confidence: 99%

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

et al. 2020

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“…HRW + Cd treatment also significantly increased in the activity and related gene expressions of APX , DHAR , monodehydroascorbate reductase ( MDHAR ), and GR . Additionally, HRW + Cd treatment increased the contents of osmotic adjustment substances, as well as the activities of POD and PPO, while significantly decreasing IAAO activity [34]. These results confirm that H 2 induces adventitious rooting under Cd stress by decreasing the oxidative damage, increasing osmotic adjustment substance content, and regulating rooting-related enzyme activity.…”

Section: Functions Of Small-molecule Compounds During Adventitiousmentioning

confidence: 61%

“…Also, exogenous H 2 dramatically increased leaf chlorophyll content, Fv/Fm, ɸPSII, and qP as well as the activities of SOD, POD, CAT, and APX enzymes during drought [18]. Additionally, a recent study showed that under cadmium (Cd) stress, 50% HRW was the most proper dose to trigger adventitious rooting in cucumber [34]. Compared with Cd treatment, HRW + Cd treatment significantly reduced the content of malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), superoxide radical (O 2 − ), thiobarbituric acid reactive substances (TBARS), ascorbic acid (AsA), and reduced glutathione (GSH), as well as relative electrical conductivity (REC), lipoxygenase (LOX) activity, AsA/docosahexaenoic acid (DHA) ratio, and GSH/oxidized glutathione (GSSG) ratio, while increasing DHA and GSSG content.…”

Section: Functions Of Small-molecule Compounds During Adventitiousmentioning

confidence: 99%

“…Schematic model of the interactions among different small signaling molecules during plant adventitious rooting through regulating different plant physiological process systems (antioxidant system, water balance system, and photosynthetic system). In the antioxidant system, NO is regarded as the downstream molecule to enhance the enzyme activities of SOD, POD, APX, and MDA and the production of O 2 − and H 2 O 2 [18,22,23,29,34]. In the water balance system, NO is also located downstream to increase RWC, WSC, stomatal aperture, and electrolyte leakage of plants [5,18,28].…”

Section: Figurementioning

confidence: 99%

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Roles of Small-Molecule Compounds in Plant Adventitious Root Development

Deng

Wang

et al. 2019

Biomolecules

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Adventitious root (AR) is a kind of later root, which derives from stems and leaf petioles of plants. Many different kinds of small signaling molecules can transmit information between cells of multicellular organisms. It has been found that small molecules can be involved in many growth and development processes of plants, including stomatal movement, flowering, fruit ripening and developing, and AR formation. Therefore, this review focuses on discussing the functions and mechanisms of small signaling molecules in the adventitious rooting process. These compounds, such as nitric oxide (NO), hydrogen gas (H2), hydrogen sulfide (H2S), carbon monoxide (CO), methane (CH4), ethylene (ETH), and hydrogen peroxide (H2O2), can be involved in the induction of AR formation or development. This review also sums the crosstalk between these compounds. Besides, those signaling molecules can regulate the expressions of some genes during AR development, including cell division genes, auxin-related genes, and adventitious rooting-related genes. We conclude that these small-molecule compounds enhance adventitious rooting by regulating antioxidant, water balance, and photosynthetic systems as well as affecting transportation and distribution of auxin, and these compounds further conduct positive effects on horticultural plants under environmental stresses. Hence, the effect of these molecules in plant AR formation and development is definitely a hot issue to explore in the horticultural study now and in the future.

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“…Purified H 2 gas (99.99%, v/v) generated from a hydrogen gas generator (QL-300, Saikesaisi Hydrogen Energy Co., Ltd, Shandong, China) was bubbled into 1000 mL distilled water solution at a rate of 330 mL min −1 for 60 min. Then, the corresponding hydrogen-rich water (HRW) was rapidly diluted to the different saturations required (1, 10, 50 and 100%, [v/v]) (Wang et al 2019a ). The dissolved hydrogen portable meter was used to analyze the H 2 concentration in freshly prepared HRW, and it was maintained at an effective concentration level at 25 °C for at least 12 h (Figs.…”

Section: Methodsmentioning

confidence: 99%

Hydrogen-rich water promotes the formation of bulblets in Lilium davidii var. unicolor through regulating sucrose and starch metabolism

Hou

Wang

et al. 2021

Planta

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Main conclusion HRW increased the content of starch and sucrose via regulating a series of sucrose and starch synthesis genes, which induced the formation of bulblets and adventitious roots of Lilium davidii var. unicolor. Abstract Hydrogen gas (H 2 ), as a signaling molecule, has been reported to be involved in plant growth and development. Here, the effect of hydrogen-rich water (HRW) on the formation of bulblets and adventitious roots in the scale cuttings of Lilium davidii var. unicolor and its mechanisms at the molecular levels were investigated. The results revealed that compared with distilled water treatment (Con), the number of bulblets and adventitious roots were significantly promoted by different concentrations of HRW treatment. Treatment with 100% HRW obtained the most positive effects. RNA sequencing (RNA-seq) analysis found that compared with Con, a total of 1702 differentially expressed genes (DEGs, upregulated 552 DEGs, downregulated 1150 DEGs) were obtained under HRW treatment. The sucrose and starch metabolism, cysteine and methionine metabolism and phenylalanine metabolism were significantly enriched in the analysis of the Kyoto encyclopedia of genes and genomes (KEGG). In addition, the genes involved in carbohydrate metabolism were significantly upregulated or downregulated (upregulated 22 DEGs, downregulated 15 DEGs), indicating that starch and sucrose metabolism held a central position. The expressions of 12 DEGs were identified as coding for key enzymes in metabolism of carbohydrates was validated by qPCR during bulblet formation progress. RNA-seq analysis and expression profiles indicated that the unigene levels such as glgc, Susy, otsA and glgP, BMY and TPS were well correlated with sucrose and starch metabolism during HRW-induced bulblet formation. The change of key enzyme content in starch and sucrose metabolism pathway was explored during bulblet formation in Lilium under HRW treatment. Meanwhile, compared with Con, 100% HRW treatment increased the levels of sucrose and starch, and decreased the trehalose content, which were agreed with the expression pattern of DEGs related to the biosynthesis pathway of sucrose, starch and trehalose. Therefore, this study suggested that HRW could promote the accumulation of sucrose and starch contents in mother scales, and decreased the trehalose content, this might provide more energy for bulblet formation. Supplementary Information The online version contains supplementary material available at 10.1007/s00425-021-03762-6.

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Hydrogen gas promotes the adventitious rooting in cucumber under cadmium stress

Cited by 22 publications

References 48 publications

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

Roles of Small-Molecule Compounds in Plant Adventitious Root Development

Hydrogen-rich water promotes the formation of bulblets in Lilium davidii var. unicolor through regulating sucrose and starch metabolism

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