Identification and characterization of lactic acid bacteria associated with tropical grass silage produced in Okinawa

Hanagasaki, Takashi

doi:10.17138/tgft(8)234-249

Cited by 4 publications

(1 citation statement)

References 3 publications

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“…Stackebrandt and Goebel (2006) reported that a 16S rDNA sequence similarity range of above 98.7%–99% should be mandatory for testing the genomic uniqueness of novel isolates; species characterized by having lower than 98.7% of 16S rDNA sequence similarity with other type strains should be considered as new species, although there could be some exceptional cases. In Okinawa, which consists of many islands in the subtropical region, unique microflora may exist, and several kinds of microorganisms from this region have unique characteristics (Hanagasaki, 2020). Accordingly, it is quite likely that the isolates in this study are new species with unique characteristics.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Erwinia sp. causing black rot of papaya (Carica papaya) first recorded in Okinawa Main Island, Japan

Hanagasaki

Yamashiro²,

Gima

et al. 2021

Plant Pathology

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Since 2002, papaya black rot has been spreading over several islands of Okinawa Prefecture, Japan. To devise a prevention strategy for the disease, microbiological research on the pathogen was conducted. Twelve strains were isolated from papaya infected with black rot showing symptoms such as water‐soaked lesions on stems and petioles, black spots on fruits, and rotted leaves turning yellow with necrotic spots. Through Koch's postulates, we confirmed that the isolated strains caused papaya black rot. Bacteriological assays showed that the strains have characteristics different from the type strains of Erwinia mallotivora, E. papayae, and E. psidii. Moreover, 16S rDNA sequence similarity searches showed that the isolated strains had less than 98.6% similarity with type strains. Additionally, phylogenetic analysis of 16S rDNA sequences suggested that the isolated strains were possibly a novel species belonging to the genus Erwinia, as the strains formed an independent cluster and had low sequence similarity with the type strains. Earlier studies indicated that papaya black rot is caused by E. cypripedii. Therefore, we propose to add the Erwinia sp. isolated in this study to the list of papaya black rot pathogens.

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Section: Discussionmentioning

confidence: 99%

Characterization of Erwinia sp. causing black rot of papaya (Carica papaya) first recorded in Okinawa Main Island, Japan

Hanagasaki

Yamashiro²,

Gima

et al. 2021

Plant Pathology

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Biological soil disinfestation prevented the development of Fusarium vanleeuwenii root rot on lisianthus plants on ethanol-irrigated soil in Okinawa, Japan

Ajitomi,

Hanagasaki,

Chinen

et al. 2024

Fruits

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Fermentation Quality of Silages Produced from Wilted Sown Tropical Perennial Grass Pastures with or without a Bacterial Inoculant

Piltz¹,

Meyer²,

Brennan

et al. 2022

Agronomy

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High growth rates and rapid reproductive development and associated decline in feed quality of sown tropical perennial grass pastures present management challenges for livestock producers. Conservation of surplus forage as silage could be an effective management tool. Experiments were conducted to evaluate the fermentation quality of silages produced from tropical grasses. Five species (Chloris gayana, Megathyrsus maximus, Panicum coloratum, Digitaria eriantha and Cenchrus clandestinus) were ensiled without additives after a short, effective wilt at dry matter (DM) contents ranging from 302.4 to 650.1 g kg−1. The fermentation profile of all silages in 2019 was typical for high DM silages, but in 2020 ammonia (% of total nitrogen: NH3-N), acetic acid and pH levels were higher. In 2020 M. maximus (302.4 g kg−1 DM) was poorly preserved with 20.2% NH3-N. The DM content of all other silages exceeded 350 g kg−1 and fermentation quality was generally good. In a second experiment, M. maximus was ensiled at 365 g kg−1 chopped and 447 g kg−1 DM chopped and unchopped, either without or with Pioneer 1171® (Lactobacillus plantarum and Enterococcus faecium) or Lallemand Magniva Classic® (L. plantarum and Pediococcus pentasaceus) bacterial inoculant. Inoculants increased lactic acid production, reduced pH and improved fermentation compared to Control, but D-lactate, L-lactate and acetic acid production differed between inoculants. Unchopped silages had higher pH and NH3-N and better preserved protein fraction than chopped silages at the same DM content. In both experiments, wilting increased water soluble carbohydrates by 0.5–31.5 g kg−1 DM and ensiling increased degradation of the protein fraction. We concluded that a rapid and effective wilt combined with a bacterial additive resulted in well preserved tropical grass silages.

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Identification and characterization of lactic acid bacteria associated with tropical grass silage produced in Okinawa

Cited by 4 publications

References 3 publications

Characterization of Erwinia sp. causing black rot of papaya (Carica papaya) first recorded in Okinawa Main Island, Japan

Characterization of Erwinia sp. causing black rot of papaya (Carica papaya) first recorded in Okinawa Main Island, Japan

Biological soil disinfestation prevented the development of Fusarium vanleeuwenii root rot on lisianthus plants on ethanol-irrigated soil in Okinawa, Japan

Fermentation Quality of Silages Produced from Wilted Sown Tropical Perennial Grass Pastures with or without a Bacterial Inoculant

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