Identification of candidate genes associated with senescence in durum wheat (Triticum turgidum subsp. durum) using cDNA-AFLP

Abstract

Senescence is an integrated response of plants to various internal (developmental) and external (environmental) signals. It is a highly regulated process leading eventually to the death of cells, single organs such as leaves, or even whole plants. In cereals, which are monocarpic plants, senescence represents the final stage of development. In order to study senescence in durum wheat (Triticum turgidum subsp. durum), a cDNA-AFLP analysis was performed. The transcription profiles of plants at different developmental stages (flowering and senescent) were compared. About 2000 cDNA fragments, ranging in size from 160 to 1900 bp, were reproducibly detected. This allowed the identification of 57 differentially expressed cDNAs corresponding to genes belonging to different functional categories related to cellular metabolism, transcription, maintenance of DNA structure, transport and signal transduction. This paper reports the identification of novel durum wheat candidate genes involved in the senescence process, and provides new information about the senescence programme of this important crop species.

Transcriptomic and proteomic analyses of a pale-green durum wheat mutant shows variations in photosystem components and metabolic deficiencies under drought stress

“…HvPAP14 also generates mature forms of storage proteins in seeds, provides free amino acids during germination [71], and confers stress tolerance and defense against pathogens [72,73]. TdS40 is induced during post-flowering senescence in wheat [74], and also by JA, SA and in chlorotic/necrotic leaf tissue following infection with Pyrenophora teres [75]. …”

Application of Salicylic Acid Improves Filling of Inferior Grains of Rice during Late Maturity under Mild Cold Stress

“…Leaf senescence is the final stage of leaf development and is critical for grain‐yield formation because it affects the assimilate supply and plant fitness. However, leaf premature senescence is induced by many abiotic stresses, such as drought, cold stress, or damaging radiation, which decrease grain yield (Yoshida 2003; Rampino et al, 2011). A good deal of evidence has been provided on the favorable virtues of delayed leaf senescence, which is characterized by enhanced photosynthesis, increased chlorophyll, and soluble protein contents, and the decreased degradation of organelles could contribute to higher grain weights and grain yields (Jibran et al, 2013; Rubia et al, 2014; Zhang et al, 2015).…”

Molecular Analysis of Differentially Expressed Genes in Birch (Betula Platyphylla) Inflorescence

“…Moreover, when compared to powerful cDNA-microarray technology, this approach is much cheaper and readily applies to all species. Some floral-related genes in other plants, such as Petunia hybrida (5), Camellia sinensis (14), Brassica nigra (13), and Triticum turgidum (16), were screened, cloned, and well characterized by this method. The cDNA-AFLP approach retrieved useful information on gene expression and on changes thereof between male and female inflorescence of birch.…”