Importance of ligand structure in DNA/protein binding, mutagenicity, excision repair and nutritional aspects of chromium(<scp>iii</scp>) complexes

Vaidyanathan, V. G.; Asthana, Yamini; Nair, Balachandran Unni

doi:10.1039/c2dt32124f

Cited by 10 publications

(4 citation statements)

References 66 publications

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“…Chromium can exist in nine different oxidation states, with Cr 3+ and Cr(VI) (CrO 4 2– or Cr 2 O 7 2– ) being the most stable forms. While Cr 3+ is considered as a nutritional supplement, Cr(VI) is highly carcinogenic . Cr(VI) readily enters cells by anion transport mechanisms, and its toxicity is attributed to DNA damage.…”

Section: Introductionmentioning

confidence: 99%

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Cr³⁺ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis

Zhou

Vazin

et al. 2016

Inorg. Chem.

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The interaction between chromium ions and DNA is of great interest in inorganic chemistry, toxicology, and analytical chemistry. Most previous studies focused on in situ reduction of Cr(VI), producing Cr 3+ for DNA binding. Recently, Cr 3+ was reported to activate the Ce13d DNAzyme for RNA cleavage. Herein, the Ce13d is used to study two types of Cr 3+ and DNA interactions. First, Cr 3+ binds to the DNA phosphate backbone weakly through reversible electrostatic interactions, which is weakened by adding competing inorganic phosphate. On the other hand, Cr 3+ coordinates with DNA nucleobases forming stable crosslinks that can survive denaturing gel electrophoresis condition. The binding of Cr 3+ to different nucleobases was further studied in terms of binding kinetic and affinity by exploiting FAM-labeled DNA homopolymers. Once binding takes place, the stable Cr 3+ /DNA complex cannot be dissociated by EDTA, attributable to the ultra-slow ligand exchange rate of Cr 3+ . The binding rate follows the order of G > C >T ≈ A. Finally, Cr 3+ gradually loses its DNA binding ability after storing at neutral or high pH, attributable hydrolysis. This hydrolysis can be reversed by lowering the pH.This work provides a deeper insight into the bioinorganic chemistry of Cr 3+ coordination with DNA, clarifies some inconsistency in the previous literature, and offers practically useful information for generating reproducible results.3

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Section: Introductionmentioning

confidence: 99%

“…While Cr 3+ is considered as a nutritional supplement, 1 Cr(VI) is highly carcinogenic. 2 Cr(VI) readily enters cells by anion transport mechanisms, and its toxicity is attributed to DNA damage.…”

Section: Introductionmentioning

confidence: 99%

Cr³⁺ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis

Zhou

Vazin

et al. 2016

Inorg. Chem.

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“…Several attempts have been made to explain the nature of the DNA–Cr 3+ interactions. Phosphate groups and/or the N7 guanine atoms have been indicated as the interaction sites in DNA–Cr 3+ adducts [ 2 , 5 – 8 ]. Some nucleotide–Cr 3+ and DNA–[Cr(Schiff base)] complexes were characterized by different techniques, such as 31 P NMR, EPR, UV–Vis and IR spectroscopy; however, those studies provided little, if any, precise structural data [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

High-resolution crystal structure of Z-DNA in complex with Cr3+ cations

et al. 2015

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This work is part of our project aimed at characterizing metal-binding properties of left-handed Z-DNA helices. The three Cr3+ cations found in the asymmetric unit of the d(CGCGCG)2–Cr3+ crystal structure do not form direct coordination bonds with atoms of the Z-DNA molecule. Instead, the hydrated Cr3+ ions are engaged in outer-sphere interactions with phosphate groups and O6 and N7 guanine atoms of the DNA. The Cr3+(1) and Cr3+(2) ions have disordered coordination spheres occupied by six water molecules each. These partial-occupancy chromium cations are 2.354(15) Å apart and are bridged by three water molecules from their hydration spheres. The Cr3+(3) cation has distorted square pyramidal geometry. In addition to the high degree of disorder of the DNA backbone, alternate conformations are also observed for the deoxyribose and base moieties of the G2 nucleotide. Our work illuminates the question of conformational flexibility of Z-DNA and its interaction mode with transition-metal cations.Electronic supplementary materialThe online version of this article (doi:10.1007/s00775-015-1247-5) contains supplementary material, which is available to authorized users.

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“…Deoxyribonucleic acid (DNA) is an important genetic substance in organisms and quite often the main molecular target for studies under the presence of pollutants . Pollutants exert their carcinogenic and teratogenic action by mutating DNA . Many chemical substances could interact with DNA, and change the process of photoinduced charge transfer.…”

Section: Introductionmentioning

confidence: 99%

Interaction between Microbes DNA and Atrazine in Black Soil Analyzed by Spectroscopy

Zhang

Wang

Guo

et al. 2015

CLEAN Soil Air Water

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It could be shown that atrazine bound to microbes DNA in black soil by using ethidium bromide (EB) as a fluorescence probe. To study the interaction between atrazine and the microbes DNA in black soil, spectral absorption and fluorescence analysis were adopted. Atrazine, when bound to DNA, showed a hyperchromic and red shift in the absorption spectra and fluorescence quenching (>50%) in the fluorescence spectra, which indicated the intercalation. The effect of phosphate ion showed that there were electrostatic attractions with the anionic sugar‐phosphate backbone of DNA. The ratio plots “fluorescence intensities in the absence” to “fluorescence intensities in the presence of a quencher” (Fo/F) showed that the quenching of fluorescence by atrazine was a combined quenching process. The results indicated that there were two binding modes, intercalation and electrostatic attractions, happened simultaneously. The hyperchromicity in the absorption spectra indicated the breaking or denaturation of DNA, and a fluorescence inhibiting effect showed that atrazine had a high ability to bind to the DNA of black soil microbes. Based on these results, it was speculated that the binding between atrazine and the DNA the black soil microbes could inhibit some functions of the nucleic acid, restrain microbial activity or cause genotoxicity.

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Importance of ligand structure in DNA/protein binding, mutagenicity, excision repair and nutritional aspects of chromium(iii) complexes

Cited by 10 publications

References 66 publications

Cr³⁺ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis

Cr³⁺ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis

High-resolution crystal structure of Z-DNA in complex with Cr3+ cations

Interaction between Microbes DNA and Atrazine in Black Soil Analyzed by Spectroscopy

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Importance of ligand structure in DNA/protein binding, mutagenicity, excision repair and nutritional aspects of chromium(iii) complexes

Cited by 10 publications

References 66 publications

Cr3+ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis

Cr3+ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis

High-resolution crystal structure of Z-DNA in complex with Cr3+ cations

Interaction between Microbes DNA and Atrazine in Black Soil Analyzed by Spectroscopy

Contact Info

Product

Resources

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Cr³⁺ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis

Cr³⁺ Binding to DNA Backbone Phosphate and Bases: Slow Ligand Exchange Rates and Metal Hydrolysis