Improved light-induced cell detachment on rutile TiO2 nanodot films

Cheng, Kui; Sun, Yu; Wan, Hongping; Wang, Xiaozhao; Weng, Wenjian; Lin, Jun; Wang, Huiming

doi:10.1016/j.actbio.2015.08.026

Cited by 21 publications

(25 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bai et al obtained the similar proliferation trend of MC3T3-E1 cells on anatase/rutile, anatase and amorphous TiO 2 nanotubes as the result of Yu et al [ 36 ]. Cheng et al noted that the anatase TiO 2 nanodot film presented a higher proliferation rate of NIH3T3 cells than the nanodots with anatase/rutile or rutile phase [ 37 ]. Our result indicates that the crystallized TiO 2 surfaces were able to improve the cell proliferation compared to the amorphous surface, especially the anatase/rutile surface displayed the highest cell proliferation rate, which was probably associated with the physicochemical properties of the prepared surfaces.…”

Section: Resultsmentioning

confidence: 99%

Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation

Jiang

Zhang

et al. 2021

Bioactive Materials

View full text Add to dashboard Cite

Surface structures and physicochemical properties critically influence osseointegration of titanium (Ti) implants. Previous studies have shown that the surface with both micro- and nanoscale roughness may provide multiple features comparable to cell dimensions and thus efficiently regulate cell-material interaction. However, less attention has been made to further optimize the physicochemical properties (e.g., crystalline phase) and to further improve the bioactivity of micro/nanostructured surfaces. Herein, micro/nanostructured titania surfaces with different crystalline phases (amorphous, anatase and anatase/rutile) were prepared and hydroxyapatite (HA) nanorods were deposited onto the as-prepared surfaces by a spin-assisted layer-by-layer assembly method without greatly altering the initial multi-scale morphology and wettability. The effects of crystalline phase, chemical composition and wettability on osteoblast response were investigated. It is noted that all the micro/nanostructured surfaces with/without HA modification presented superamphiphilic. The activities of MC3T3-E1 cells suggested that the proliferation trend on the micro/nanostructured surfaces was greatly influenced by different crystalline phases, and the highest proliferation rate was obtained on the anatase/rutile surface, followed by the anatase; but the cell differentiation and extracellular matrix mineralization were almost the same among them. After ultrathin HA modification on the micro/nanostructured surfaces with different crystalline phases, it exhibited similar proliferation trend as the original surfaces; however, the cell differentiation and extracellular matrix mineralization were significantly improved. The results indicate that the introduction of ultrathin HA to the micro/nanostructured surfaces with optimized crystalline phase benefits cell proliferation, differentiation and maturation, which suggests a favorable biomimetic microenvironment and provides the potential for enhanced implant osseointegration in vivo.

show abstract

Section: Resultsmentioning

confidence: 99%

Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation

Jiang

Zhang

et al. 2021

Bioactive Materials

View full text Add to dashboard Cite

show abstract

“…Moreover, TiO 2 nanodots and UV365 illumination are safe for cell sheets. However, only a few cell types of cell sheets (MC3T3 [11] and NIH3T3 [12]) have been developed with this technology. In this study, rBMCSs were chosen to develop cell sheets using light-induced cell sheet technology.…”

Section: Introductionmentioning

confidence: 99%

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Jiang

Lai

et al. 2017

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.

show abstract

“…Thus, it could be suggested that sensitization of CQDs enhanced charge separation of TiO 2 and simultaneously trapped the transferred electrons on the surface. According to our previous study on the mechanism of protein responses under UV illumination, such electrons accumulation on the surface could increase the negative potential of the film surface . It has been reported that the negative charge is related to the protein conformation change from the α helix to β sheet .…”

Section: Resultsmentioning

confidence: 98%

“…Currently,t he majority of cell-sheet harvesting is obtained by thermo-responsive systems. [1,2,4,10,11] Nevertheless, other strategies, such as electricity-induced, [12] pH-change-induced, [13] magnetic-force-induced, [14] bioresorbable-layers-induced, [15] light-induced, [16][17][18] and chemistry-induced [19] cell-sheet detachment have been successfully developed in recent years. In fact, rare cell-releasing processes might occur along with an impact on cell functions by regulation of biomolecules in cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

“…TiO 2 occurs naturallyi nt hree crystalline forms:a natase, rutile, and brookite. [21] Sun et al [17] reported that crystalline phases of TiO 2 could affect light-induced cell detachment and the rutile phase contributes to the improved cell-release behavior. Moreover,W an et al [18] reported that the porousn anostructure of TiO 2 exhibits improved cell-detachment per-Carbon quantum dots (CQDs) have been incorporated as asensitizer into TiO 2 films to regulate the light-induced cell-detachment performance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Light‐Induced Cell‐Sheet Harvest on TiO₂ Films Sensitized with Carbon Quantum Dots

et al. 2016

Self Cite

View full text Add to dashboard Cite

Carbon quantum dots (CQDs) have been incorporated as a sensitizer into TiO2 films to regulate the light‐induced cell‐detachment performance. Depending on the amount of CQDs incorporated, nanodots or porous films are obtained. It was found that the right amount of CQDs incorporation significantly improves the cell‐sheet detachment efficiency, and an intact cell sheet could be obtained after merely five minutes of UV illumination (365 nm, UV365), which is much faster than that from CQDs‐free TiO2 film. Furthermore, cell sheets on CQDs‐sensitized TiO2 films show improved viability, and alkaline phosphatase and collagen I activity either before or after light‐induced detachment. The improved cell‐detachment performance is ascribed to the effective inhibition of charge recombination of the TiO2 film and the microstructure variations, which increase the negative surface potential and change the secondary structure of the adsorbed extracellular protein molecules; this results in a faster release of cells. It is suggested that the faster release reduces the possible detrimental impact on cell functions during detachment and helps in maintaining the excellent cellular activity. This study demonstrates that CQDs sensitization is an effective approach for optimizing the performance of light‐induced cell‐sheet harvest.

show abstract

Improved light-induced cell detachment on rutile TiO2 nanodot films

Cited by 21 publications

References 36 publications

Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation

Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Light‐Induced Cell‐Sheet Harvest on TiO₂ Films Sensitized with Carbon Quantum Dots

Contact Info

Product

Resources

About

Improved light-induced cell detachment on rutile TiO2 nanodot films

Cited by 21 publications

References 36 publications

Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation

Hydroxyapatite-modified micro/nanostructured titania surfaces with different crystalline phases for osteoblast regulation

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Light‐Induced Cell‐Sheet Harvest on TiO2 Films Sensitized with Carbon Quantum Dots

Contact Info

Product

Resources

About

Light‐Induced Cell‐Sheet Harvest on TiO₂ Films Sensitized with Carbon Quantum Dots