In vitro bioactivity of 3D microstructure hydroxyapatite/collagen based‐egg white as an antibacterial agent

Abstract

The present study aims to design 3D scaffold hydroxyapatite (HA)/collagen (Coll) based egg‐white (EW) as antibacterial properties. The calcium source in HA synthesis derived from the Pinctada maxima shell cultivated on Bali Island has proven biocompatibility, and the compressive strength exceeded human bone. HA synthesis by precipitation with heat treatment in oven‐dried at 80°C (HA‐80) and annealed at 900°C (HA‐900), has crystallinity 48% and 85%, respectively, were used for scaffold design. The physicochemical properties of X‐ray diffractometer spectra showed that increasing temperature affected the crystallinity and HA phase formed. Furthermore, the crystal structure of HA changed in nanocomposite due to the substitution of Coll and EW, and the Fourier transform infrared spectroscopy spectra confirmed that the absorption peak of the phosphate group (1027–1029 cm−1) decreased intensity, presumably by protein binding of EW and Coll. The cell viability of HA/Coll/EW in 24, 48, and 72 h incubation period was 112.34 ± 4.36, 104.89 ± 3.41, 72.88 ± 6.85, respectively. The decreases of cell viability due to high cell density and reduced nutrients in wells. Antibacterial activity of HA/Col/EW exhibited a strong zone of inhibition against bacteria causing periodontitis; Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Staphylococcus aureus.

Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material

“…Dislocations in the material are observed with prominent XRD peaks influenced by increasing temperature and decreasing crystal size. The success of the antibacterial gel is seen from the characterization of XRD with an amorphous peak, following the references made by previous studies [29,31]. The amorphous phase produced broad XRD peaks at low 2 theta angles of 10-30 degrees because the sample does not have crystalline properties.…”

Mechanical Characteristics and Bioactivity of Nanocomposite Hydroxyapatite/Collagen Coated Titanium for Bone Tissue Engineering

“…Figure 2 shows the characteristic of HAp after calcination at 1000 °C for 2h. The FTIR spectrum ( Figure 2 a) identified the presence of OH, carbonate, and phosphate groups at 3642 cm −1 , 1412 cm −1 , and 1018 cm −1 −960 cm −1, respectively [ 21 ].…”

“…However, after SBF immersion (3 days), the traces of amino acids derived from collagen were indicated by absorption peaks at 1631–1648 cm −1 and 1519–1521 cm −1 corresponding to protein amide I and amide II [ 34 ]. The bands at 1440–1467 cm −1 correspond to carbonate, and at 1051–1052 cm −1 correspond to phosphate [ 21 , 35 , 36 ]. The presence of organic components in the surface layer is manifested by minor peaks between 3000 and 2800 cm −1 and 1500 and 1200 cm −1 [ 37 , 38 ].…”

“…The porosity/surface porosity of the coating was determined based on SEM images. The SEM images were first converted into two-dimensional grayscale matrices, and all procedures were performed using the instructions provided by the OriginPro software [ 10 , 21 , 44 , 45 ]. The pore distribution and solid volume of coating are evaluated on the base of color distribution; solid volume is shown by red color, and pore distribution is shown by blue color ( Figure 9 ).…”

“…The preparation and synthesis of HAp are based on previous studies [ 21 ]. Briefly, HAp synthesis by the precipitation method was carried out using CaO extracted from the P. maxima shell.…”