In-vitro regeneration of olive tree by somatic embryogenesis

Trabelsi, E. B.; Bouzid, Sadok; Bouzid, M.; Elloumi, Nedra; Belfeleh, Zina; Benabdallah, Amel; Ghezel, Rachida

doi:10.1007/bf03030446

Cited by 16 publications

(19 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Olive SE is restricted to few cultivars using juvenile explants such as cotyledonary tissues (e.g., Trabelsi et al 2003), zygotic embryos (e.g., Leva et al 1995), and leaf blades, leaf petioles, and hypocotyls of germinated seeds (Shibli et al 2001). Only Rugini and Caricato (1995) succeeded in inducing indirect SE from petioles of adult plants after previous rejuvenation by in vitro culture.…”

Section: Introductionmentioning

confidence: 98%

Somatic embryogenesis induction in leaves and petioles of a mature wild olive

Capelo

Silva

Brito

et al. 2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

We describe a protocol for somatic embryogenesis (SE) induction from an adult wild olive tree (Olea europaea ssp. europaea var. sylvestris. The protocol used confirms for the first time that there is no need to use juvenile or rejuvenated material for SE induction. For SE induction, petiole and leaf (proximal, intermediary and distal zones) explants were grown on Murashige and Skoog (MS) or Olive Medium (OM) media with different combinations of plant growth regulators (PGR): a-naphthaleneacetic acid (NAA), Zeatin (Zea), indole-3-butyric acid (IBA), 2-isopentyl adenine (2iP), thidiazuron (TDZ) and 6-benzylaminopurine (BAP). All media had 30 g/l sucrose and 7 g/l agar, and the pH was adjusted to 5.8. Cultures were incubated in the dark and, after 3 months, they were transferred to MS medium without PGR for expression. Petiole explants gave the highest callus production, while for SE induction and expression distal blade leaf and petiole explants gave the highest rates. The best medium for SE induction was MS with 12.25 lM IBA plus 4.56 lM Zea. Histological analyses confirmed the individuality of globular somatic embryos. This is the first report of SE expression in explants without rejuvenation in Olea genus, and opens perspectives for using this strategy in SE protocols both for this wild genotype and for commercial genotypes.

show abstract

Section: Introductionmentioning

confidence: 98%

Somatic embryogenesis induction in leaves and petioles of a mature wild olive

Capelo

Silva

Brito

et al. 2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…In O. europaea, somatic embryogenesis has been induced mostly in juvenile material from cotyledonary tissues (Orinos and Mitrakos 1991;Leva et al 1995;Trabelsi et al 2003), immature zygotic embryos (Rugini 1988;Leva et al 1995) and mature embryo callus (Orinos and Mitrakos 1991;Mitrakos et al 1992). Only Rugini and Caricato (1995) succeeded in inducing SE from leaf petiole tissues of adult plants using a method which they designated 'double regenerating system' and which requires previous rejuvenation by in vitro shoot culture.…”

Section: Introductionmentioning

confidence: 98%

“…Top scale indicates fragment size in nucleotides. Left scale indicates fluorescence intensity measured in relativeTrabelsi et al 2003). OnlyGarcía-Férriz et al (2002) …”

mentioning

confidence: 97%

Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

Lopes

Capelo

Brito

et al. 2008

Trees

View full text Add to dashboard Cite

The crop species Olea europaea L. (olive tree) is of great economic importance in the Mediterranean region. Hence, many efforts have been done in the last decades to propagate this commercially valuable species by in vitro methods. On the other hand, the lesser known Olea maderensis (Lowe) Rivas Mart. & Del Arco which is a native species of the Madeira Archipelago has only been the subject of micropropagation from nodal stem cuttings. Therefore, in this work we analysed the stability of ten nuclear simple sequence repeat (SSR) markers at successive stages of the somatic embryogenesis process in two adult trees belonging to these two species from the Madeira Archipelago. For the induction of somatic embryogenesis, petiole and leaf explants were cultivated on solid Murashige and Skoog medium (MS) with 12.25 lM indole-3-butyric acid (IBA) and 4.56 lM of zeatin, in the dark. After 3 months, different callus tissues (non-embryogenic, pre-embryogenic and embryogenic) developed from leaf explants and petioles were later transferred to MS medium without growth regulators in the dark. All ten SSR markers were able to distinguish between species. However, no mutations were found at the SSR loci at any of the successive developmental stages from PEMs (pre-embryogenic masses) to somatic embryos. This genetic uniformity was observed within material derived from each genotype/species and its respective donor plant. Therefore, we conclude that the genomic integrities of both O. europaea and O. maderensis were maintained throughout the stages of the embryogenic processes in study suggesting the absence of somaclonal variation.

show abstract

“…Várias técnicas de micropropagação de oliveira têm sido reportadas por diversos autores, sendo por segmentos nodais (Santos et al, 2003), cotilédones (Brhadda et al, 2003), embriogênese somática (Trabelsi et al, 2003) ou microenxertia (Revilla et al, 1996;Peyvandi et al, 2010). Entretanto, alguns autores citam a importância de inúme-ros fatores, como idade do explante ou genótipo, no sucesso da micropropagação (Rugini & Caricato, 1995;Leva et al, 2002).…”

Section: Introductionunclassified

Otimização de um protocolo para micropropagação da oliveira Ascolano 315

Villa

Pasqual²,

Freitas³

2010

Rev. Ceres

View full text Add to dashboard Cite

Optimization of a protocol for the micropropagation of olive tree cv. Ascolano 315Micropropagation can be a viable technique for the multiplication of olive trees. The objective of this work was to induce multiplication in explants of olive tree. Nodal segments with 2 cm length, without leaves, derived from in vitro plantlets of cultivar Ascolano 315 were excised and inoculated in test tubes. The tubes contained 15 mL of OM (Olive medium) culture medium supplemented with 2 g L -1 of activated charcoal, 4 concentrations of 6-benzilaminopurin (BAP) and 4 concentrations of coconut water and solidified with 5.5 g L -1 of agar. The pH was adjusted to 5.8 before medium sterilization at 121ºC and 1 atm for 20 min. The experimental design was complete randomized in a 4 x 4 factorial scheme. The explants were kept in a growth room 25±1ºC, light intensity of 32 ì mol m -2 s -1 and photoperiod of 16 hours, for 70 days. The culture medium OM added of 1.0 mg L -1 of BAP and 100 mL L -1 of coconut water provided the greatest length and biomass of the aerial part. The largest number of roots was obtained with 0.5 mg L -1 of BAP associated with 25 mL L -1 of coconut water.

show abstract

In-vitro regeneration of olive tree by somatic embryogenesis

Cited by 16 publications

References 27 publications

Somatic embryogenesis induction in leaves and petioles of a mature wild olive

Somatic embryogenesis induction in leaves and petioles of a mature wild olive

Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

Otimização de um protocolo para micropropagação da oliveira Ascolano 315

Contact Info

Product

Resources

About