Influence of cryoprotectants glycerol and amides, combined with antioxidants on quality of frozen-thawed boar sperm

Fertility of boar semen cryopreserved in extender supplemented with butylated hydroxytoluene

“…Another fact is that AI with FT boar semen still results in low conception rate and small litter sizes [3,19]. The lifespan of FT boar semen in the female reproductive tract is shorter (2-8 hours) than that of its liquid-preserved counterpart [20].…”

“…The relatively low fertility of FT boar semen is associated with many factors. It has been reported that reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage [3]. An important indicator of the membrane damage is a change in membrane permeability, such as increased permeability to stains and a release of intracellular substances.…”

Cryopreservation of Porcine Gametes, Embryos and Genital Tissues: State of the Art

“…While such interval does not affect cryosurvival in "good-freezer" boars, those considered moderate or bad freezers benefit from a minimum of 3% glycerol (Hernandez et al 2007a). A broad range of other solutes (mostly alcohols, sugars, diols and amides) have also been tested for CPA capacity (Fuller 2004, Buranaamnuay et al 2011, but boar spermatozoa react variably. Alcohols and diols can induce membrane blebbing.…”

Cryopreservation of Piau‐Breed Wild Boar Sperm: Assessment of Cooling Curves and Centrifugation Regimes

“…The results regarding correlations with the complementary tests used to assess sperm quality have been controversial, most likely resulting from individual differences in sperm responses to specific types of cryoprotectants and/or freezing protocols used, as indicated by Buranaamnuay et al. () and Pinho et al. (), and few studies have reported high values of correlation between semen traits or functional sperm responses and fertility in vivo .…”