Inhibitory effects of methanol extracts of selected plants on proliferation of two human melanoma cell lines

Fraihat, Alaa; Alatrash, Luma; Abbasi, Reem; Abu‐Irmaileh, Bashaer; Hamed, Saja; Mohammad, Mohammad; Abu-rish, Eman Y.; Bustanji, Yasser

doi:10.4314/tjpr.v17i8.25

Cited by 6 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These studies revealed anti-diabetic, anti-oxidant, anti-inflammatory as well as anti-tumor activities of plant extracts and isolates. These effects were mainly attributed to plant phenolics, flavonoids, coumarins, terpenes and carotenoids, such as rosmarinic acid (phenolic) and carnosic acid (CA, terpene) [3,4,5,6,7]. In this study, we focused on two Salvia species, Salvia fruticosa Mill.…”

Section: Introductionmentioning

confidence: 99%

“…(SF) and Salvia pomifera L. (SP), which are used in Crete as traditional medicines, however clinical evidence for these traditional indications in accordance with EBM (Evidence Based Medicine) is lacking. In particular, SF plants, which are the most important sage in the United States, have shown a cytotoxic effect in several cancer cell lines in vitro, including human colon, pancreatic, breast, and cervical carcinoma and also leukemic and melanoma cell lines [2,3,4,6,7,8,9]. As with other Salvia species, SF chemoprotective properties are cell line dependent and several mechanisms for the molecular pathways’ activation have been proposed, such as DNA damage prevention, enhanced DNA repair, cell proliferation inhibition and apoptosis induction [5,10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Phenolic Compounds and Antiproliferative Effects of Salvia pomifera and Salvia fruticosa Extracts

et al. 2019

View full text Add to dashboard Cite

The phenolic compounds of methanolic extracts of Salvia pomifera and Salvia fruticosa were identified by liquid chromatography tandem mass spectrometry. Carnosic acid and its metabolite carnosol were the most abundant terpene phenolic compounds of S. fruticosa, while they were completely absent in S. pomifera. The main terpene phenolic constituent of S. pomifera was 12-O-methylcarnosic acid and its mass/mass fragmentation pathway was explained. The detailed mechanism of carnosic acid oxidation to carnosol was suggested. The effects of Salvia extracts and/or carnosic acid, the main diterpene phenolic component of S. fruticosa, on the proliferation and cell cycle of two melanoma cell lines (A375, Mel JuSo) and human fibroblast cell line (HFF) were investigated by MTT assay, PI-exclusion assay and flow cytometry cell cycle analysis. Extract of S. fruticosa more efficiently than S. pomifera extract reduced the proliferation of the human melanoma cells. Carnosic acid showed the most significant effect. The first evidence that carnosic acid affects microtubule dynamics and arrests the cell cycle in the G2/M phase was provided. Collectively, our results demonstrate that these two Salvia species are plants of medicinal interest with perspective for further investigation. Carnosic acid could be the compound responsible for the biological activities of S. fruticosa extracts.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Phenolic Compounds and Antiproliferative Effects of Salvia pomifera and Salvia fruticosa Extracts

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Salvia fruticosa metanol ekstraktlarının, melanom hücre hattı WM 136.1A proliferasyonu üzerinde doza bağlı olarak anlamlı bir sitotoksik etki gösterdiği bildirilmiştir [80].…”

Section: Antikanser Etkileriunclassified

Salvia fruticosa’nın (Anadolu Adaçayı) Terapötik Etkileri

Elmas

2021

International Journal of Life Sciences and Biotechnology

View full text Add to dashboard Cite

ÖZET Anadolu adaçayı (Salvia fruticosa), Lamiaceae familyasına ait bir bitki olup, Anadolu'da geleneksel halk hekimliğinde yaygın olarak kullanılmaktadır. Bitkinin yaprakları çay şeklinde tüketilir ve yapraklarından uçucu yağ elde edilir. Salvia fruticosa bitkisi farmasötik ajan olarak (antioksidan, antikanser, antimikrobiyal, antifungal, antihiperglisemik, antihipertansif vb.) kullanımı dışında aromaterapi, parfümeri ve kozmetik sektöründe; yiyeceklerde baharat, aroma verici ve koruyucu olarak pek çok alanda kullanıma sahiptir. Ancak bitkinin irrasyonel kullanımı ciddi yan etkilere ve komplikasyonlara yol açmaktadır. Bu derleme, Salvia fruticosa'nın botanik özelliklerini, uçucu yağ bileşenlerini, bu bileşenlerin potansiyel terapötik etkilerini ve neden olabileceği olası toksik etkileri değerlendirmeyi amaçlamaktadır.

show abstract

“…Melanoma is considered as the most aggressive type of skin cancer, and patients with local or distant metastasis of melanoma have a poor prognosis [1]. The treatment for melanoma remains a challenge due to pre-existing or acquired drug resistance [2].…”

Section: Introductionmentioning

confidence: 99%

CD147 promotes melanoma cell growth via SOX4-mediated glycolytic metabolism

Sun¹,

Yang²,

Jiang³

2021

Trop. J. Pharm Res

View full text Add to dashboard Cite

Purpose: To determine the functional roles of cluster of differentiation 147 (CD147) in glycolysis in melanoma cells.Methods: CD147 expression in melanoma tissue and adjacent normal tissue was determined using quantitative real time polymrase chain reaction (qRT-PCR) and immunohistochemistry. Cell Counting Kit-8 (CCK-8) and colony formation assays were used to evaluate cell viability and colony formation, respectively. The role of CD147 in glycolysis in melanoma cells was investigated by determining glucose uptake, production of lactate, and cellular level of ATP.Results: CD147 was enhanced more in melanoma tissue than that in the adjacent normal tissue (p < 0.001). CD147 overexpression promoted the viability and colony formation of melanoma cells. On the other hand, CD147 silencing decreased the viability and colony formation of melanoma cells. Glucose uptake, production of lactate, and cellular level of ATP were upregulated in melanoma cells by CD147 overexpression and downregulated by shRNA-mediated depletion of CD147. CD147 increased expression of C-X-C motif chemokine ligand 1 (CXCL1) to activate the sex-determining region Y-related high-mobility group box 4 (SOX4) pathway. Knockdown of CXCL1 attenuated the positive regulatory effect of CD147 on SOX4. Besides, overexpression of SOX4 reversed the suppressive effects of CD147 silencing on melanoma cell viability, colony formation, and glycolysis.Conclusion: CD147 contributes to melanoma cell growth via upregulation of SOX-mediated glycolysis, thus providing a therapeutic avenue for the management of melanoma. Keywords: Cluster of differentiation 147, CD147, Sex-determining region Y-related high-mobility group box 4, Melanoma, Cell growth, Glycolysis

show abstract

Inhibitory effects of methanol extracts of selected plants on proliferation of two human melanoma cell lines

Cited by 6 publications

References 10 publications

Characterization of Phenolic Compounds and Antiproliferative Effects of Salvia pomifera and Salvia fruticosa Extracts

Characterization of Phenolic Compounds and Antiproliferative Effects of Salvia pomifera and Salvia fruticosa Extracts

Salvia fruticosa’nın (Anadolu Adaçayı) Terapötik Etkileri

CD147 promotes melanoma cell growth via SOX4-mediated glycolytic metabolism

Contact Info

Product

Resources

About