Intermediate filaments and IF-associated proteins: from cell architecture to cell proliferation

Nishimura, Yuhei; Kasahara, Kousuke; Inagaki, Masaki

doi:10.2183/pjab.95.034

Cited by 28 publications

(23 citation statements)

References 180 publications

(223 reference statements)

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“…Knockdown of fatty acid synthase, a transcriptional target of SREBP1, also restored primary cilia to the prostate cancer cell line, LNCaP [100]. Treatment with rapamycin, an mTORC1 inhibitor, restored the impaired ciliogenesis of prostate cancer cell lines (PC3 and DU145) and inhibited their proliferation [101], which is consistent with studies demonstrating that primary cilia work as a brake on cell proliferation [6][7][8][9][10]63,64,66,102].…”

Section: Primary Cilia and Lipid Raft-mediated Akt Signalling In Cancer Biologysupporting

confidence: 84%

“…Primary cilia are non-motile, 1-10 µm long antenna-like structures observed in a variety of vertebrate cells. Primary cilia sense extracellular signals, such as biochemical and mechanical stimuli, and transduce these signals into the cell to regulate functions such as differentiation and proliferation [1][2][3][4][5][6][7][8][9][10][11]. Primary cilia convey a wide range of signals, including the signalling pathways of hedgehog, receptor tyrosine kinases (RTKs) and G protein-coupled receptors such as lysophosphatidic acid receptors [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Primary cilia and lipid raft dynamics

et al. 2021

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Primary cilia, antenna-like structures of the plasma membrane, detect various extracellular cues and transduce signals into the cell to regulate a wide range of functions. Lipid rafts, plasma membrane microdomains enriched in cholesterol, sphingolipids and specific proteins, are also signalling hubs involved in a myriad of physiological functions. Although impairment of primary cilia and lipid rafts is associated with various diseases, the relationship between primary cilia and lipid rafts is poorly understood. Here, we review a newly discovered interaction between primary cilia and lipid raft dynamics that occurs during Akt signalling in adipogenesis. We also discuss the relationship between primary cilia and lipid raft-mediated Akt signalling in cancer biology. This review provides a novel perspective on primary cilia in the regulation of lipid raft dynamics.

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Section: Primary Cilia and Lipid Raft-mediated Akt Signalling In Cancer Biologysupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Primary cilia and lipid raft dynamics

et al. 2021

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“…Although additional studies in primary cells and tissue specimens need to be carried out to confirm these results, vimentin also seems to coincide with the signal of acetylated tubulin in images from primary rat cardiac fibroblasts and human tissue depicted in a recent report [56]. Some previously known connections between intermediate filaments and primary cilium include the regulatory role of several intermediate filament-associated proteins, including trichoplein and filamin A in ciliogenesis [79,80], as well as the presence of a net of intermediate filaments around basal bodies [81]. Nevertheless, to the best of our knowledge, the participation of vimentin in cilium architecture had not been underscored.…”

Section: Discussionmentioning

confidence: 60%

Immunolocalization studies of vimentin and ACE2 on the surface of cells exposed to SARS-CoV-2 Spike proteins

Lalioti

González-Sanz

Lois-Bermejo

et al. 2021

Preprint

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The Spike protein from SARS-CoV-2 mediates docking of the virus onto cells and contributes to viral invasion. Several cellular receptors are involved in SARS-CoV-2 Spike docking at the cell surface, including ACE2 and neuropilin. The intermediate filament protein vimentin has been reported to be present at the surface of certain cells and act as a co-receptor for several viruses; furthermore, its potential involvement in interactions with Spike proteins has been proposed. Here we have explored the binding of Spike protein constructs to several cell types using low-temperature immunofluorescence approaches in live cells, to minimize internalization. Incubation of cells with tagged Spike S or Spike S1 subunit led to discrete dotted patterns at the cell surface, which showed scarce colocalization with a lipid raft marker, but consistent coincidence with ACE2. Under our conditions, vimentin immunoreactivity appeared as spots or patches unevenly distributed at the surface of diverse cell types. Remarkably, several observations including potential antibody internalization and adherence to cells of vimentin-positive structures present in the extracellular medium exposed the complexity of vimentin cell surface immunoreactivity, which requires careful assessment. Notably, overall colocalization of Spike and vimentin signals markedly varied with the cell type and the immunodetection sequence. In turn, vimentin-positive spots moderately colocalized with ACE2; however, a particular enrichment was detected at elongated structures positive for acetylated tubulin, consistent with primary cilia, which also showed Spike binding. Thus, these results suggest that vimentin-ACE2 interaction could occur at selective locations near the cell surface, including ciliated structures, which can act as platforms for SARS-CoV-2 docking.

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“…To divide, cells must actively generate forces to accommodate their shape changes and overcome mechanical constraints by the surrounding tissue. The IF network reorganizes extensively not only to adapt to but also to promote these changes [24][25][26][27]. As cells round up to facilitate the accurate positioning of the spindle and the correct segregation of chromosomes, cortical tension generated by the actin cortex increases.…”

Section: Intermediate Filaments Adapt Cell Mechanics To Cell Behaviormentioning

confidence: 99%

Intermediate Filaments from Tissue Integrity to Single Molecule Mechanics

Bodegraven

Etienne-Manneville

2021

Cells

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Cytoplasmic intermediate filaments (IFs), which together with actin and microtubules form the cytoskeleton, are composed of a large and diverse family of proteins. Efforts to elucidate the molecular mechanisms responsible for IF-associated diseases increasingly point towards a major contribution of IFs to the cell’s ability to adapt, resist and respond to mechanical challenges. From these observations, which echo the impressive resilience of IFs in vitro, we here discuss the role of IFs as master integrators of cell and tissue mechanics. In this review, we summarize our current understanding of the contribution of IFs to cell and tissue mechanics and explain these results in light of recent in vitro studies that have investigated physical properties of single IFs and IF networks. Finally, we highlight how changes in IF gene expression, network assembly dynamics, and post-translational modifications can tune IF properties to adapt cell and tissue mechanics to changing environments.

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Intermediate filaments and IF-associated proteins: from cell architecture to cell proliferation

Cited by 28 publications

References 180 publications

Primary cilia and lipid raft dynamics

Primary cilia and lipid raft dynamics

Immunolocalization studies of vimentin and ACE2 on the surface of cells exposed to SARS-CoV-2 Spike proteins

Intermediate Filaments from Tissue Integrity to Single Molecule Mechanics

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