Investigation of the potential of agro-industrial material as low cost substrate for ethanol production by using Candida tropicalis and Zymomonas mobilis

Patle, Sonali; Lal, Banwari

doi:10.1016/j.biombioe.2007.12.008

Cited by 48 publications

(24 citation statements)

References 16 publications

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“…In this case are used enzymes such as glucoamylase (amyloglucosidase) obtained from Aspergillus niger or Rhizopus sp. These enzymes hydrolyze the chemical bonds α-(1-4) and α- (1)(2)(3)(4)(5)(6), to obtain products such as maltose or D-glucose syrups with DE around 40 and 96, respectively. 1,[10][11][12] These amylolytic enzymes commonly are used in soluble solutions and they can be susceptible to inhibition for both reaction substrates and/or products.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, it is easily produced in tropical and sub-tropical zones, mainly in Asia, South-America and South-Africa. 3 For obtaining glucose from cassava starch, an amylose/ amylopectin ratio of 18/82% is hydrolyzed by an amylolytic enzymatic complex. [4][5][6] These conventional processes are carried out in three steps: gelatinization, liquefaction and saccharification.…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic hydrolysis of cassava starch for production of bioethanol with a colombian wild yeast strain

Ruiz¹,

Sánchez²,

Torrres³

et al. 2011

J. Braz. Chem. Soc.

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A hidrólise enzimática do amido de mandioca para produção de xaropes de glucose foi avaliada usando alfa-amilase de Bacillus licheniformis e glucoamilase de Aspergillus níger. Também, uma mistura enzimática composta de α-amylase de Aspergillus kawachi e glucoamilase de Aspergillus níger foi testada. As condições da enzima para a hidrólise do amido foram otimizadas por um planejamento fatorial experimental (3 3 ×2) usando como variáveis a concentração do substrato, a relação enzima/ substrato e o tempo de reação. As condições ótimas de reação com 100 g de amido per L foram: α-amilase pH 5,0, 80 °C e 130,5 U g -1 de amido; glucoamilase pH 4,5, 70 °C e 81,5 U g -1 de amido. Adicionalmente, as condições ótimas da mistura enzimática foram pH 4,5, 46 °C e 16,4 U g -1 de amido. Finalmente, a produção de álcool usando xaropes de glucose a partir do amido hidrolisado enzimaticamente foi realizada usando uma cepa selvagem de Candida sp isolada do caldo de cana de açúcar, obtendo produtividades em etanol volumétrico em torno de 1,Enzymatic hydrolysis of cassava starch for producing glucose syrups was evaluated using alpha-amylase from Bacillus licheniformis and glucoamylase from Aspergillus niger. Moreover, an enzyme mixture of α-amylase from Aspergillus kawachi and glucoamylase from Aspergillus niger was tested. Enzyme conditions for starch hydrolysis were optimized by a factorial experimental design (3 3 ×2) using as variables substrate concentration, enzyme/substrate ratio and time reaction. Optimal enzyme reactions with 100 g of starch per L were: α-amylase at pH 5.0, 80 °C and enzyme dosage of 130.5 U g -1 of starch; and glucoamylase, pH 4.5, 70 °C and enzyme dosage of 81.5 U g -1 of starch. Additionally, optimal conditions for the enzymatic mixture were pH 4.5, 46 °C, and enzyme dosage of 16.4 U g -1 of starch. Finally, alcohol production using glucose syrups from enzymatically-hydrolyzed starch was carried out with a wild strain of Candida sp isolated from sugar cane juice, obtaining volumetric ethanol productivities around 1.8-3.2 g L -1 h -1 .

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enzymatic hydrolysis of cassava starch for production of bioethanol with a colombian wild yeast strain

Ruiz¹,

Sánchez²,

Torrres³

et al. 2011

J. Braz. Chem. Soc.

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show abstract

“…It has been used successfully in co-culture fermentations. A recent study (Patle and Lal 2008) showed that the ethanol production from a waste product of tapioca processing, thippi, using Zymomonas mobilis and C. tropicalis independently, produced 83% and 77% ethanol, respectively. Using a mixed culture, the production increased to 93% at a concentration of 72.8 g/L in 48 h.…”

Section: Aspergillusmentioning

confidence: 99%

Using microorganisms to brew biofuels

Rao

Dufour

Swana

2011

In Vitro Cell.Dev.Biol.-Plant

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Interest in alternative fuel sources has grown in recent years in response to a confluence of factors, including concerns over our reliance on and increasing demand for fossil fuels as well as the deleterious environmental effects of fossil fuel extraction and utilization. The use of microbederived fuel alcohols is a viable alternative, as they are renewable, emit fewer greenhouse gasses, and require little augmentation of current energy infrastructure as compared to other sustainable transportation options such as electric vehicles and fuel cells. Here, we present a brief overview of candidate substrates for alcohol production with a focus on lignocellulosic sources, relevant microorganisms under research for industrialization and the biotechnological techniques used to improve alcohol production phenotypes.

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“…Foram específicos para cada micro-organismo, conforme literatura: S. cerevisiae, 30 °C e 4,5 (TOSETTO, 2008 e PARK, 2010); Z. mobilis, 30 °C e 6,0 (PATLE & LAL, 2008;YANASE et al, 2012e MAITI et al, 2011PINILLA et al, 2011); P. stipitis, 32 °C e 5,5 (SLININGER et al, 1990apud OLIVEIRA, 2010; P. tannophilus, 30 °C e 6,0 (CONVERTI et al, 2011;SATHESCH-PRABU & MURUGESAN, 2011).…”

Section: Temperatura E Phunclassified

Determinação Da Eficiência Alcoólica De Diferentes Micro-Organismos Visando a Produção De Etanol 2g

Montagnoli¹,

Just²,

Sellin³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -O etanol apresenta uma alternativa viável como combustível a partir de vias fermentativas, reconhecido como bioetanol. O bioetanol é produzido por microorganismos fermentadores de carboidratos, os quais podem ser derivados de materiais lignocelulósicos, possuindo a glicose como um dos principais açúcares desta matéria-prima. Neste trabalho avaliaram-se a cinética de consumo do substrato e a produção de etanol por quatro diferentes micro-organismos fermentadores (Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis e Pachysolen tannophilus) para o ensaio padrão, empregando a glicose como fonte de carbono. As fermentações em mistura sintética foram realizadas em Erlenmeyers com 80% (v/v) de meio de cultivo e 20% de inóculo. Para a análise das amostras, retiradas periodicamente, foram realizadas a determinação de açúcares redutores (AR) e de etanol (P). O melhor valor de conversão de substrato em produto foi encontrado para o micro-organismo Z. mobilis (0,45 g/g).

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Investigation of the potential of agro-industrial material as low cost substrate for ethanol production by using Candida tropicalis and Zymomonas mobilis

Cited by 48 publications

References 16 publications

Enzymatic hydrolysis of cassava starch for production of bioethanol with a colombian wild yeast strain

Enzymatic hydrolysis of cassava starch for production of bioethanol with a colombian wild yeast strain

Using microorganisms to brew biofuels

Determinação Da Eficiência Alcoólica De Diferentes Micro-Organismos Visando a Produção De Etanol 2g

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